Isolation and cultivation of neural stem cells from the embryonic rat brain and spinal cord

Acta Physiologica Sinica

Volumn 55, Issue 3, 2003, Pages 278-283

  Fu, Sai Li ^a   Ma, Zheng Wen ^a   Yin, Lan ^a   Lu, Pei Hua ^a   Xu, Xiao Ming ^a,b  

^a SHANGHAI JIAO TONG UNIVERSITY SCHOOL OF MEDICINE   (China)

^b University of Louisville School of Medicine   (United States)

Author keywords

Central neural system; Embryo; Epidermal growth factor; Fibroblast growth factor 2; Neural stem cell

Indexed keywords

EPIDERMAL GROWTH FACTOR; FIBROBLAST GROWTH FACTOR 2;

ANIMAL CELL; ARTICLE; ASTROCYTE; BRAIN CELL; CELL ISOLATION; CONTROLLED STUDY; CULTURE MEDIUM; EMBRYO; EMBRYO CELL; IMMUNOCYTOCHEMISTRY; NERVE CELL CULTURE; NERVE CELL DIFFERENTIATION; NONHUMAN; OLIGODENDROGLIA; RAT; SPINAL CORD; STEM CELL;

ANIMALS; BRAIN; CELL CULTURE TECHNIQUES; CELL SEPARATION; CELLS, CULTURED; EMBRYO, MAMMALIAN; EMBRYONIC STEM CELLS; FEMALE; NEURAL STEM CELLS; PREGNANCY; RATS; RATS, WISTAR; SPINAL CORD;

EID: 0042155351     PISSN: 03710874     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (10)

1. Iannotti C, Lü XB, Lu PH, Xu XM. Neural stem cell transplantation in the repair of spinal cord injury. Pro Natural Sci 2001; 11 (7): 490-503.
2. Reynolds BA, Weiss S. Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science 1992; 255 (5052): 1707-1710
3. Carpenter MK, Cui X, Hu ZY, Jackson J, Sherman S, Seiger A, Wahlberg LU. In vitro expansion of a multipotent population of human neural progenitor cells. Exp Neurol 1999;158:265-278.
4. Quinn SM, Walters WM, Vescovi AL, Whittemore SR. Lineage restriction of neuroepithelial precursor cells from fetal human spinal cord. J Neurosci Res 1999;57:590-602.
5. Shihabuddin LS, Ray J, Gage FH. FGF-2 is sufficient to isolate progenitors found in the adult mammalian spinal cord. Exp Neurol 1997;148:577-586.
6. Johe KK, Hazel TG, Muller T, Dugich-Djordjevic MM, Mckay RDG. Single factors direct the differentiation of stem cells from the fetal and adult central nervous system. Gene Dev 1996;10:3129-3140.
7. Whittemore SR, Morassutti DJ, Walters WM, Liu RH, Magnuson DSK. Mitogen and substrate differentially affect the lineage restriction of adult rat subventricular zone neural precursor cell populations. Exp Cell Res 1999; 252: 75-95.
8. Kallos MS, Behie LA, Vescovi AL. Extended serial passaging of mammalian neural stem cells in suspension bioreactors. Biotech Bioeng 1999; 65 (5): 589-599.
9. Kuhn HG, Winkler J, Kempermann G, Thal LJ, Gage FH. Epidermal growth factor and fibroblast growth factor-2 have different effects on neural progenitors in the adult rat brain. J Neurosci 1997; 17 (15): 5820-5829.
10. Kitchens DL, Snyder EY, Gottlieb DI. FGF and EGF are mitogens for immortalized neural progenitors. J Neurobio 1994; 25 (7): 797-807.