Two-layer antibody capture of enzymes on the surface of microtiter plates: Application to the study of the regulation of phospholipase C-γ1 catalytic activity

Analytical Biochemistry

Volumn 320, Issue 2, 2003, Pages 193-198

  Buckley, Colin T ^a   Caldwell, Kevin K ^a  

^a UNIVERSITY OF NEW MEXICO HEALTH SCIENCES CENTER   (United States)

Author keywords

Activity; Affinity capture; Enzyme; Microtiter; Phospholipase C

Indexed keywords

AMINO ACIDS; ANTIBODIES; CATALYST ACTIVITY; ENZYMES; ISOENZYMES; PURIFICATION; THERMODYNAMIC PROPERTIES;

AFFINITY CAPTURE; ANTIBODY CAPTURE; HIGH THROUGHPUT PROCEDURES; MICROTITER; MICROTITER PLATES; PHOSPHOLIPASE C; PURIFIED ENZYME; SELECTIVE AGENTS;

ENZYME ACTIVITY;

IMMUNOGLOBULIN ANTIBODY; PHOSPHOLIPASE C; PROTEIN TYROSINE KINASE; STREPTAVIDIN;

ACCURACY; ANIMAL TISSUE; ARTICLE; BIOTINYLATION; BRAIN TISSUE; CATALYSIS; CELL LYSATE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME PHOSPHORYLATION; FEMALE; IMMUNOCHEMISTRY; MICROTITER PLATE ASSAY; NONHUMAN; PRIORITY JOURNAL; RAT; REGULATORY MECHANISM; REPRODUCIBILITY; VALIDATION PROCESS;

EID: 0042121381     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(03)00394-4     Document Type: Article

References (12)

1. Majerus P.W., Ross T.S., Cunningham T.W., Caldwell K.K., Jefferson A.B., Bansal V.S. Recent insights in phosphatidylinositol signaling. Cell. 63:1990;459-465.
2. Williams R.L. Mammalian phosphoinositide-specific phospholipase C. Biochim. Biophys. Acta. 1441:1999;255-267.
3. Rhee S.G. Regulation of phosphoinositide-specific phospholipase C. Annu. Rev. Biochem. 70:2001;281-312.
4. Watanabe M., Nakamura M., Sato K., Kano M., Simon M.I., Inoue Y. Patterns of expression for the mRNA corresponding to the four isoforms of phospholipase Cβ in mouse brain. Eur. J. Neurosci. 10:1998;2016-2025.
5. Homma Y., Takenawa T., Emori Y., Sorimachi H., Suzuki K. Tissue- and cell type-specific expression of mRNAs for four types of inositol phospholipid-specific phospholipase C. Biochem. Biophys. Res. Commun. 164:1989;406-412.
6. Lee S.B., Rhee S.G. Molecular cloning, splice variants, expression, and purification of phospholipase C-δ4. J. Biol. Chem. 271:1996;25-31.
7. Kelley G.G., Reks S.E., Ondrako J.M., Smrcka A.V. Phospholipase Cε: a novel Ras effector. EMBO J. 20:2001;743-754.
8. Wahl M.I., Jones G.A., Nishibe S., Rhee S.G., Carpenter G.J. Growth factor stimulation of phospholipase C-gamma 1 activity. Comparative properties of control and activated enzymes. J. Biol. Chem. 267:1992;10447-10456.
9. Weeber E.J., Savage D.D., Sutherland R.J., Caldwell K.K. Fear conditioning-induced alterations of phospholipase C-β1a protein level and enzyme activity in rat hippocampal formation and medial frontal cortex. Neurobiol. Learn. Mem. 76:2001;151-182.
10. Bradford M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
11. Suter M., Butler J.E. The Immunochemistry of sandwich ELISAs. II. A novel system prevents the denaturation of capture antibodies. Immunol. Lett. 13:1986;313-316.
12. Peterman J.H., Tarcha P.J., Chu V.P., Butler J.E. The immunochemistry of sandwich-ELISAs. IV. The antigen capture capacity of antibody covalently attached to bromoaceyl surface-functionalized polystyrene. J. Immunol. Methods. 111:1988;271-275.