Purification of soluble human epidermal growth factor (hEGF) from recombinant Escherichia coli culture broth by using expanded-bed adsorption chromatography

Biotechnology and Applied Biochemistry

Volumn 38, Issue 1, 2003, Pages 9-13

  Lee, Yoon Seok ^a   Suh, Chang Woo ^b   Park, Seung Kook ^a   Lee, Eun Kyu ^b  

^a Daewoong Pharmaceutical Co Ltd   (South Korea)

^b Hanyang University   (South Korea)

Author keywords

Escherichia coli fermentation; Expanded bed adsorption (EBA); Human epidermal growth factor (hEGF); Protein purification; Scale up

Indexed keywords

ADSORPTION; BIOMASS; HIGH PRESSURE LIQUID CHROMATOGRAPHY; ION EXCHANGE; PURIFICATION;

CELLULAR BIOMASS;

ESCHERICHIA COLI;

GAMMA UROGASTRONE; RECOMBINANT PROTEIN;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BACTERIUM CULTURE; CATION EXCHANGE; ESCHERICHIA COLI; EXPANDED BED CHROMATOGRAPHY; NONHUMAN; PROTEIN PURIFICATION; PROTEIN SECRETION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; STATISTICAL SIGNIFICANCE;

ADSORPTION; CHROMATOGRAPHY; CHROMATOGRAPHY, AGAROSE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, ION EXCHANGE; CULTURE MEDIA; EPIDERMAL GROWTH FACTOR; ESCHERICHIA COLI; FERMENTATION; HUMANS; RECOMBINANT PROTEINS; SOLUBILITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0042090308     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20020113     Document Type: Article

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