Seasonal detection of pear decline phytoplasma by nested-PCR in different pear cultivars

Plant Pathology

Volumn 52, Issue 4, 2003, Pages 513-520

  Garcia Chapa, M ^a   Medina, V ^b   Viruel, M A ^a   Lavina A ^a   Batlle, A ^a  

^a IRTA   (Spain)

^b UNIVERSITY OF LLEIDA   (Spain)

Author keywords

Detection; Grafting; Nested PCR; Pyrus communis; Transmission

Indexed keywords

CULTIVAR; DISEASE; PHYTOPLASMA; POLYMERASE CHAIN REACTION;

BACTERIA (MICROORGANISMS); CANDIDATUS PHYTOPLASMA PYRI; PHYTOPLASMA; PYRUS COMMUNIS;

EID: 0042072888     PISSN: 00320862     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-3059.2003.00868.x     Document Type: Article

References (34)

1. Ahrens U, Seemüller E, 1992. Detection of DNA of plant pathogenic mycoplasmalike organisms by a polymerase chain reaction that amplifies a sequence of the 16S rRNA gene. Phytopathology 82, 828-32.
2. Avinent LL, Lácer G, Almacellas J, Torà R, 1997. Pear decline in Spain. Plant Pathology 46, 694-8.
3. Batlle A, Laviña A, Garcia M, Sanchez I, Sabater J, Medina V, 2001. Incidence, detection and epidemiology of pear decline in Northeastern Spain. Proceedings of the 5th Congress of the European Foundation for Plant Pathology Biodiversity in Plant Pathology, 2000. Taormina, Italy: SIVP, 196-9.
4. Blodget EC, Aichele MD, Parsons JL, 1963. Evidence of a transmissible factor in pear decline. Plant Disease Reporter 47, 89-93.
5. Braun E, Sinclair W, 1976. Histopathology of phloem necrosis in Ulmus americana. Phytopathology 66, 598-607.
6. Daire X, Boudon-Padieu E, Berville A, Schneider B, Caudwell A, 1992. Cloned DNA probes for detection of grapevine flavescence doree mycoplasma-like organism (MLO). Annals of Applied Biology 121, 95-103.
7. Davies DL, Guise CM, Clark MF, Adams NA, 1992. Parrys's disease of pears is similar to pear decline and associated with mycoplasma-like organisms transmitted by Cacopsylla pyricola. Plant Pathology 41, 194-203.
8. Del Serrone P, Starza SL, Krystai L, Kolber M, Barba M, 1998. Occurrence of apple proliferation and pear decline phytoplasmas in diseased pear trees in Hungary. Journal of Plant Pathology 80, 53-8.
9. Deng S, Hiruki C, 1991. Amplification of 16s rRNA genes from culturable and nonculturable mollicutes. Journal of Microbiology Methods 14, 53-61.
10. Evert RF, 1960. Phloem structure in Pyrus communis L. and its seasonal changes. University Of California Publications in Botany 32, 127-94.
11. Giunchedi L, Poggi Pollini C, Biondi S, Babini AR, 1994. PCR detection of MLOs in quick decline-affected pear trees in Italy. Annals of Applied Biology 124, 399-403.
12. Green MJ, Thompson DA, MacKenzie DJ, 1999. Easy and efficient DNA extraction from woody plants for the detection of phytoplasmas by polymerase chain reaction. Plant Disease 83, 482-5.
13. Griggs WH, 1965. Topgrafting potted trees. In: Progress Report on Pear Decline. Berkeley, USA: University of California, 10-1.
14. Hibino H, Schneider H, 1970. Mycoplasmalike bodies in sieve tubes of pear trees affected with pear decline. Phytopathology 60, 499-501.
15. Jarausch W, Lansac M, Dosba F, 1999. Seasonal colonization pattern of European stone fruit yellows phytoplasmas in different Prunus species detected by specific PCR. Journal of Phytopathology 147, 47-54.
16. John E, Maliyakal, 1992. An efficient method for isolation of RNA and DNA from plants containing polyphenolics. Nucleic Acids Research 20, 2381.
17. Kunze L, 1972. Untersuchungen zum Nachwels der Triebsucht des Apfels im Serientest. Mitellungen der Biologischen Bundesanstalt für Land und Forwirtschaff 144, 35-46.
18. Lorenz KH, Schneider B, Ahrens U, Seemüller E, 1995. Detection of the apple proliferation and pear decline phytoplasmas by PCR amplification of ribosomal and nonribosomal DNA. Phytopathology 85, 771-6.
19. Malinowski T, Zandarski J, Komorowska B, Zawadzka B, 1996. Detection of pear decline phytoplasma in declining pear trees in Poland. Plant Disease 80, 464.
20. Marcone C, Ragozzino A, Cirulli M, 1999. Widespread occurrence of pear decline disease in southern Italy. Informatore Fitopatologico 49, 50-2.
21. Rallo L, 1973. Decaimiento del peral en plantaciones del Valle del Ebro. Anales INIA. Protección Vegetale 3, 147-205.
22. Rosenberg DA, Jones AL, 1977. Seasonal variation in infectivity of inoculum from X-diseased peach and choke-cherry plants. Plant Disease Reporter 61, 1022-4.
23. Schaper U, 1981. Untersuchungen zum Nachweis und Besiedlungsverhalten sowie zur Bekämpfung der Erreger der Triebsucht des Apfels und des Birnenverfalls. Dissertation. Göttingen, Germany: University of Göttingen.
24. Schaper U, Seemüller E, 1982. Condition of the phloem and the persistence of mycoplasmalike organisms associated with apple proliferation and pear decline. Phytopathology 72, 736-42.
25. Schneider H, 1970. Graft transmission and host range of pear decline causal agent. Phytopathology 60, 204-7.
26. Schneider H, 1971. Infectivity of stem scions versus root scions from pear decline-infected Pyrus sp. 'Variolosa' trees following dormancy chilling. Phytopathology 61, 1324.
27. Seemüller E, Marcone C, Lauer U, Ragozzino A, Göschl M, 1998a. Current status of molecular classification of the phytoplasmas. Journal of Plant Pathology 80, 3-26.
28. Seemüller E, Schaper U, Zimbermann E, 1984. Seasonal variation in the colonization patterns of mycoplasmalike organisms associated with apple proliferation and pear decline. Journal of Plant Diseases and Protection 91, 371-82.
29. Seemüller E, Stolz H, Kison H, 1998b. Persistence of the European stone fruit yellows phytoplasma in aerial parts of Prunus taxa during the dormant season. Journal of Phytopathology 146, 407-10.
30. Seidl V, 1965. The possibility of using root grafting method of testing for apple proliferation virus disease. Zâstita Bilja 16, 323-7.
31. Seidl V, Komarkova V, 1973. On some problems of apple proliferation disease. Proceedings of the 7th Conference of Czechoslovak Plant Virologists, 1971. Bratislava, Slovakia: Slovak Academic Science, 487-93.
32. Shalla TA, Carroll TW, Chiarappa L, 1964. Transmission of pear decline by grafting. California Agriculture 18, 4-5.
33. Smart CD, Schneider B, Blomquist CL, Guerra LJ, Harrison NA, Ahrens U, Lorenz KH, Seemüller E, Kirkpatrick B, 1996. Phytoplasma-specific PCR primers based on sequences of the 16S-23S rRNA spacer region. Applied and Environmental Microbiology 62, 2988-93.
34. Waterworth HE, Mock R, 1999. An assessment of nested PCR to detect phytoplasmas in imported dormant buds and internodal tissues of quarantined tree fruit germplasm. Plant Disease 83, 1047-50.