Heterologous expression of cDNAs encoding Chlorella sorokiniana NADP-specific glutamate dehydrogenase wild-type and mutant subunits in Escherichia coli cells and comparison of kinetic and thermal stability properties of their homohexamers

Plant Molecular Biology

Volumn 52, Issue 3, 2003, Pages 605-616

  Lawit, Shai J ^a   Miller, Philip W ^a,b   Dunn, Waltraud I ^a   Mirabile, Jeremy S ^a,c   Schmidt, Robert R ^a,d  

^a UNIVERSITY OF FLORIDA   (United States)

^b Monsanto Mystic Research   (United States)

^c Shands at Alachua General Hospital   (United States)

^d NONE   (United States)

Author keywords

Chlorella; Glutamate dehydrogenase; Nitrogen metabolism

Indexed keywords

DNA; ENZYMES; ESCHERICHIA COLI; REACTION KINETICS; SYNTHESIS (CHEMICAL); THERMODYNAMIC STABILITY;

GENETIC ENCODING;

GENETIC ENGINEERING;

AMINO ACID SEQUENCE; CHLORELLA; DIMERIZATION; DNA, COMPLEMENTARY; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GLUTAMATE DEHYDROGENASE (NADP+); IMMUNOBLOTTING; KINETICS; MOLECULAR SEQUENCE DATA; MUTATION; PROTEIN SUBUNITS; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; TEMPERATURE;

ALGAE; BACTERIA (MICROORGANISMS); CHLORELLA SOROKINIANA; ESCHERICHIA COLI; EUKARYOTA;

EID: 0041975881     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1024822312038     Document Type: Article

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