Non-invasive screening for treatment of heifers with the anabolic steroid melengestrol acetate (MGA) by feces analysis

Journal of Immunoassay and Immunochemistry

Volumn 24, Issue 3, 2003, Pages 265-272

  Lange, Iris G ^a   Daxenberger, Andreas ^a   Hageleit, Melanie ^a   Pfaffl, Michael W ^a   Meyer, Heinrich H D ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Anabolic; Feces; Melengestrol acetate; MGA; Residue analysis; Screening

Indexed keywords

ANABOLIC AGENT; MELENGESTROL ACETATE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL FOOD; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CATTLE; CONTROLLED STUDY; DRUG MONITORING; DRUG SCREENING; ENZYME LINKED IMMUNOSORBENT ASSAY; FECES ANALYSIS; FOOD INDUSTRY; NONHUMAN; PRIORITY JOURNAL; RADIOACTIVITY; RESIDUE ANALYSIS; SAMPLING; SOLID PHASE EXTRACTION;

ADMINISTRATION, ORAL; ANABOLIC AGENTS; ANIMALS; CATTLE; ENZYME-LINKED IMMUNOSORBENT ASSAY; FECES; FEMALE; FOOD CONTAMINATION; MELENGESTROL ACETATE;

EID: 0041971258     PISSN: 15321819     EISSN: None     Source Type: Journal    
DOI: 10.1081/IAS-120022936     Document Type: Article

References (20)

1. Van Puymbroeck, M.; Leyssens, L.; Vanderzande, D.; Gelan, J.; Raus, J. Metabolites in feces can be important markers for the abuse of anabolic steroids in cattle. Analyst 1998, 123, 2449-2452.
2. Van Puymbroeck, M.; Kuilman, M.E.M.; Maas, R.F.M.; Witkamp, R.F.; Leyssens, L.; Vanderzande, D.; Gelan, J.; Raus, J. Identification of some important metabolites of boldenone in urine and feces of cattle by gas chromatography-mass spectrometry. Analyst 1998, 123, 2681-2686.
3. Courtheyn, D.; Vercammen, J.; De Brabander, H.; Vandenreyt, I.; Batjoens, P.; Vanoosthuyze, K.; Van Peteghem, C. Determination of dexamethasone in urine and feces of treated cattle with negative chemical ionisation-mass spectrometry. Analyst 1994, 119, 2557-2564.
4. Courtheyn, D.; Vercammen, J.; Logghe, M.; Seghers, H.; De Wasch, K.; Van Peteghem, C. Determination of betamethasone and triamcinolone acetonide by GC-NCI-MS in excreta of treated animals and development of a fast oxidation procedure for derivatisation of corticosteroids. Analyst 1998, 123, 2409-2414.
5. Dixon, S.N.; Mallinson, C.B. Radioimmunoassay of the anabolic agent zeranol. III. Zeranol concentrations in the feces of steers implanted with zeranol (Ralgro). J. Vet. Pharmacol. Ther. 1986, 9, 88-93.
6. Jansen, E.H.; Stephany, R.W.; Vos, J.G.; Ruitenberg, E.J.; Benraad, T.J. ; de Boer, F.; de Ruig, W.G.; Weijman, J.; Schmidt, N.A. Application of diethylstilbestrol dipropionate in bulls. I. Excretion of residues in urine and feces and histological and immunohistochemical changes in the prostate. Vet. Quart. 1989, 11, 1-11.
7. Hamoir, T.; Courtheyn, D.; De Brabander, H.; Delahaut, P.; Leyssens, L.; Pottie, G. Comparison of purification procedures for the isolation and detection of anabolic residues in feces using gas chromatography-mass spectrometry. Analyst 1998, 123, 2621-2624.
8. Patterson, D.J.; Kiracofe, G.H.; Stevenson, J.S.; Corah, L.R. Control of the bovine estrus cycle with melengestrol acetate (MGA): A review. J. Anim. Sci. 1989, 67, 1895-1906.
9. Lauderdale, J.W. Use of MGA® (melengestrol acetate) in animal production. In Anabolics in Animal Production; Meissonnier, E.; Mitchell-Vigneron, J., Eds.; Office International des Epizooties: Paris, France, 1983; 193-211.
10. Bauer, E.R.S.; Daxenberger, A.; Petri, T.; Sauerwein, H.; Meyer H.H.D. Characterisation of the affinity of different anabolics and synthetic hormones to the human androgen receptor, human sex hormone binding globulin and to the bovine progestin receptor. Acta Pathol. Mic. Sci. 2000, 108, 838-846.
11. Hageleit, M.; Daxenberger, A.; Kraetzl, W.-D.; Kettler, A.; Meyer, H.H.D. (2000). Dose-dependent effects of Melengestrol Acetate (MGA) on plasma levels of oestradiol, progesterone and luteinizing hormone in cycling heifers and influences on estrogen residues in edible tissues. Acta Pathol. Mic. Sci. 2000, 108, 847-854.
12. Council of the European Communities. Council Directive (96/22/EC) of 29 April 1996 concerning the prohibition on the use in stockfarming of certain substances having hormonal or thyreostatic action and of β-agonists, and repealing Directives 81/602/EEC, 88/146/EEC and 88/299/EEC. Off. J. Eur. Commun. 1996, L 125, 3-9.
13. United States Government Printing Office, Code of Federal Regulations. Food and Drugs 21, Chapter I, Part 556.380: http://www.access.gpo.gov/nara/cfr/waisidx_99/21cfrv6_99.html.
14. Health Canada. Canada's Response to European Commission carried out to evaluate the control of residues in live animals and animal products 2000: http://www.hc-sc.gc.ca/vetdrugs-medsvet/e_canada_response.html.
15. Joint FAO/WHO Expert Committee on Food Additives, Fifty-fourth meeting, 15-24 February 2000, Summary and Conclusions. Geneva, 2000.
16. Daxenberger, A.; Meyer, K.; Hageleit, M.; Meyer, H.H.D. Detection of melengestrol acetate residues in plasma and edible tissues of heifers. Vet. Quart. 1999, 21, 154-158.
17. Hageleit, M.; Daxenberger, A.; Meyer, H.H.D. A sensitive enzyme immunoassay (ELISA) for the determination of melengestrol acetate (MGA) in adipose and muscle tissues. Food Addit. Contam. 2001, 18, 285-291.
18. Schiffer, B.; Daxenberger, A.; Meyer, K.; Meyer, H.H.D. The fate of trenbolone acetate and melengestrol acetate after application as growth promotants in cattle-Environmental studies. Environ. Health Perspect. 2001, 109, 1145-1151.
19. Metzler M.; Pfeiffer E. Genotoxic potential of xenobiotic growth promoters and their metabolites. Acta Pathol. Mic. Sci. 2001, 109, 89-95.
20. Krzeminski, L.F.; Cox, B.L.; Gosline R.E. Fate of radioactive melengestrol acetate in the bovine. J. Agric. Food Chem. 1981, 29, 387-391.