Multiplex PCRs for identification of necrotoxigenic Escherichia coli

Journal of Clinical Microbiology

Volumn 41, Issue 9, 2003, Pages 4480-4482

  Van Bost, Sigrid ^a,c   Jacquemin, Etienne ^a   Oswald, Eric ^b   Mainil, Jacques ^a  

^a UNIVERSITY OF LIÈGE   (Belgium)

^b CEMAGREF   (France)

^c Universite de Liege   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

ADHESIN; AEROBACTIN;

ARTICLE; BACTERIAL GENE; BACTERIAL VIRULENCE; BACTERIUM IDENTIFICATION; ESCHERICHIA COLI; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STRAIN DIFFERENCE;

ADHESINS, ESCHERICHIA COLI; ANIMALS; BACTERIAL TOXINS; CATTLE; CYTOTOXINS; DOGS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HUMANS; HYDROXAMIC ACIDS; POLYMERASE CHAIN REACTION; SWINE; VIRULENCE FACTORS;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0041919086     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.41.9.4480-4482.2003     Document Type: Article

References (24)

1. Berlin, Y., C. Martin, E. Oswald, and J.-P. Girardeau. 1996. Rapid and spccific detection of F17-related pilin and adhesin genes in diarrheic and septicemic Escherichia coli strains by multiplex PCR. J. Clin. Microbiol. 34:2921-2928.
2. Blum, G., V. Falbo, A. Caprioli, and J. Hacker. 1995. Gene clusters encoding the cytotoxic necrotizing factor type 1, Prs-fimbriae and α-hemolysin from the pathogenicity island associated with the presence of Vir plasmid in Escherichia coli strains. FEMS Microbiol. Lett. 58:95-100.
3. De Rycke, J., J. F. Guillot, and R. Boivin. 1987. Cytotoxins in non-enterotoxigenic strains of Escherichia coli isolated from feces of diarrheic calves. Vet. Microbiol. 15:137-150.
4. De Rycke, J., E. A. Gonzalez, J. Blanco, E. Oswald, M. Blanco, and R. Boivin. 1990. Evidence for two types of cytotoxic necrotizing factor in human and animal clinical isolates of Escherichia coli. J. Clin. Microbiol. 28:694-699.
5. De Rycke, J., A. Milon, and E. Oswald. 1999. Necrotoxic Escherichia coli (NTEC): two emerging categories of human and animal pathogens. Vet. Res. 30:221-233.
6. Falbo, V., M. Famiglietti, and A. Caprioli. 1992. Gene block encoding production of cytotoxic necrotizing factor 1 and hemolysin in Escherichia coli isolates from extraintestinal infections. Infect. Immun. 60:2182-2187.
7. Garcia, M.-I., A. Labigne, and C. Le Bouguenec. 1994. Nucleotide sequence of the afimbrial-adhesin-encoding afa-3 gene operon and its translocation via flanking IS1 insertion sequences. J. Bacteriol. 176:7601-7613.
8. Gross, R., F. Engelbrecht, and V. Braun. 1985. Identification of the genes and their polypeptide products responsible for aerobactin synthesis by pCoIV plasmids. Mol. Gen. Genet. 201:204-212.
9. Hacker, J., G. Schmidt, C. Hughes, S. Knapp, M. Marget, and W. Goebel. 1985. Cloning and characterization of genes involved in production of mannose-resistant, neuraminidase-susceptible (X) fimbriae from a uropathogenic O6:K15:H31 Eschenchia coli strain. Infect. Immun. 47:434-440.
10. Herrero, M., V. de Lorenzo, and J. B. Neilands. 1988. Nucleotide sequence of the iucD gene of the pCoIV-K30 aerobactin operon and topology of its product studied with phoA and lacZ gene fusions. J. Bacteriol. 170:56-64.
11. Johnson, J. R. 1991. Virulence factors in Escherichia coli urinary tract infection. Clin. Microbiol. Rev. 4:80-128.
12. Lalioui, L., M. Jouve, P. Gounon, and C. Le Bouguenec. 1999. Molecular cloning and characterization of the afa-7 and afa-8 gene clusters encoding afimbrial adhesins in Escherichia coli strains associated with diarrhea or septicemia in calves. Infect. Immun. 67:5048-5059.
13. Le Bouguenec, C., M. Archambaud, and A. Labigne. 1992. Rapid and specific detection of the pap, afa, and sfa adhesin-encoding operons in uropathogenic Escherichia coli strains by polymerase chain reaction. J. Clin. Microbiol. 30:1189-1193.
14. Le Bouguenec, C., and Y. Bertin. 1999. AFA and F17 adhesins produced by pathogenic Escherichia coli strains in domestic animals. Vet. Res. 30:317-342.
15. Mainil, J., E. Jacquemin, F. Hérault, and E. Oswald. 1997. Presence of pap-, sfa-, and afa-related sequences in necrotoxigenic Escherichia coli isolates from cattle: evidence for new variants of the AFA family. Can. J. Vet. Res. 61:193-199.
16. Mainil, J., E. Jacquemin, P. Pohl, J. M. Fairbrother, A. Ansuini, C. Le Bouguenec, J. De Rycke, and E. Oswald. 1999. Comparison of necrotoxigenic Escherichia coli isolates from farm animals and from humans. Vet. Microbiol. 70:123-135.
17. Mainil, J., M. Wilbaux, E. Jacquemin, E. Oswald, H. Imberechts, and S. Van Bost. 2001. Les souches pathogènes d'Escherichia coli chez les chiens et les chats: données bactériologiques et cliniques sur les souches nécrotoxinogènes et sur celles positives pour des adhésines. Ann. Med. Vet. 145:343-354.
18. Oswald, E., J. De Rycke, P. Lintermans, K. Van Muylem, J. Mainil, G. Daube, and P. Pohl. 1991. Virulence factors associated with cytotoxic necrotizing factor type two in bovine diarrheic and septicemic strains of Escherichia coli. J. Clin. Microbiol. 29:2522-2527.
19. Oswald, E., M. Sugai, A. Labigne, H. Wu, C. Fiorentini, P. Boquet, and A. O'Brien. 1994. Cytotoxic necrotizing factor type-2 produced by virulent Escherichia coli modifies the small GTP-binding proteins Rho involved in assembly of actin stress fibers. Proc. Natl. Acad. Sci. USA 91:3814-3818.
20. 2/M phase. Mol. Microbiol. 24:1095-1107.
21. Pohl, P., E. Oswald, K. Van Muylen, E. Jacquemin, P. Lintermans, and J. Mainil. 1993. Escherichia coli producing CNF1 and CNF2 cytotoxins in animals with different disorders. Vet. Res. 24:311-315.
22. Toth, I., F. Hérault, L. Beutin, and E. Oswald. 2003. Production of cytolethal distending toxins by human and animal pathogenic Escherichia coli strains: establishment of the existence of a new cdt variant (type IV). J. Clin. Microbiol. 41:4285-4291.
23. Valvano, M. A., R. P. Silver, and J. H. Crosa. 1986. Occurrence of chromosome- or plasmid-mediated aerobactin iron transport systems and hemolysin production among clonal groups of human invasive strains of Escherichia coli K1. Infect. Immun. 52:192-199.
24. Van Bost, S., M.-H. Bâbe, E. Jacquemin, and J. Mainil. 2001. Characteristics of necrotoxigenic Escherichia coli isolated from septicemic and diarrheic calves between 1958 and 1970. Vet. Microbiol. 82:311-320.