Cloning and expression of the gene for a novel protein from Mycobacterium smegmatis with functional similarity to eukaryotic calmodulin

Journal of Bacteriology

Volumn 185, Issue 17, 2003, Pages 5263-5268

  Reddy, Prasad T ^a   Prasad, C Rama ^d   Reddy, P Hemalatha ^e   Reeder, Dennis ^a   McKenney, Keith ^a   Jaffe, Howard ^b   Dimitrova, Mariana N ^c   Ginsburg, Ann ^c   Peterkofsky, Alan ^c   Murthy, P Suryanarayana ^d  

^a NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY   (United States)

^b NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE   (United States)

^c NATIONAL INSTITUTES OF HEALTH   (United States)

^d UNIVERSITY COLLEGE OF MEDICAL SCIENCES   (India)

^e UNIVERSITY OF DELHI   (India)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; BACTERIAL DNA; BACTERIAL PROTEIN; CALMODULIN ANTIBODY; CALMODULIN LIKE PROTEIN; TRIFLUOPERAZINE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BRAIN; CATTLE; DNA SEQUENCE; ENZYME ACTIVATION; ESCHERICHIA COLI; GENE EXPRESSION; GENE SEQUENCE; MOLECULAR CLONING; MOLECULAR SIZE; MYCOBACTERIUM SMEGMATIS; NONHUMAN; NUCLEOTIDE SEQUENCE; OLIGOMERIZATION; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN ISOLATION; PROTEIN PURIFICATION;

AMINO ACID SEQUENCE; ANIMALS; BACTERIAL PROTEINS; BINDING SITES; CALCIUM; CALMODULIN; CATTLE; CLONING, MOLECULAR; CONSERVED SEQUENCE; EUKARYOTIC CELLS; GENES, BACTERIAL; MOLECULAR SEQUENCE DATA; MYCOBACTERIUM SMEGMATIS; SEQUENCE ANALYSIS, DNA;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); BOS TAURUS; BOVINAE; ESCHERICHIA COLI; EUKARYOTA; MYCOBACTERIUM; MYCOBACTERIUM SMEGMATIS; UNCULTURED ACTINOMYCETE;

EID: 0041888268     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.185.17.5263-5268.2003     Document Type: Article

References (42)

1. Bloom, B. R., and C. J. Murray. 1992. Tuberculosis: commentary on a reemergent killer. Science 257:1055-1064.
2. Brodie, A. F., and C. T. Gray. 1956. Phosphorylation coupled to oxidation in bacterial extracts. J. Biol. Chem. 219:853-862.
3. Burgess, W. H., D. K. Jemiolo, and R. H. Kretsinger. 1980. Interaction of calcium and calmodulin in the presence of sodium dodecyl sulfate. Biochim. Biophys. Acta 623:257-270.
4. Burra, S. S., P. H. Reddy, S. M. Falah, T. A. Venkitasubramanian, and P. S. Murthy. 1991. Calmodulin-like protein and the phospholipids of Mycobacterium smegmatis. FEMS Microbiol. Lett. 64:189-194.
5. Burra, S. S., P. H. Reddy, and P. S. Murthy. 1995. Effect of some antitubercular drugs on the calmodulin content of Mycobacterium tuberculosis. Indian J. Clin. Biochem. 10:126-128.
6. Clarke, M., W. L. Bazari, and S. C. Kayman. 1980. Isolation and properties of calmodulin from Dictyostelium discoideum. J. Bacteriol. 141:397-400.
7. Dimitrova, M. N., R. H. Szczepanowski, S. B. Ruvinov, A. Peterkofsky, and A. Ginsburg. 2002. Interdomain interaction and substrate coupling effects on dimerization and conformational stability of enzyme I of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system. Biochemistry 41: 906-913.
8. Falah, A. M. S., R. Bhatnagar, N. Bhatnagar, Y. Singh, G. S. Sidhu, P. S. Murthy, and T. A. Venkitasubramanian. 1988. On the presence of calmodulin like protein in mycobacteria. FEMS Microbiol. Lett. 56:89-93.
9. Fernandez, J., M. DeMott, D. Atherton, and S. M. Mische. 1992. Internal protein sequence analysis: enzymatic digestion for less than 10 micrograms of protein bound to polyvinylidene difluoride or nitrocellulose membranes. Anal. Biochem. 201:255-264.
10. Fry, I. J., L. Villa, G. D. Kuehn, and J. H. Hageman. 1986. Calmodulin-like protein from Bacillus subtilis. Biochem. Biophys. Res. Commun. 134:212-217.
11. Fry, I. J., M. Becker-Hapak, and J. H. Hageman. 1991. Purification and properties of an intracellular calmodulinlike protein from Bacillus subtilis cells. J. Bacteriol. 173:2506-2513.
12. Harmon, A. C., D. Prasher, and M. J. Cormier. 1985. High-affinity calcium-binding proteins in Escherichia coli. Biochem. Biophys. Res. Commun. 127: 31-36.
13. Iwasa, Y., K. Yonemitsu, K. Matsui, K. Fukunaga, and E. Miyamoto. 1981. Calmodulin-like activity in the soluble fraction of Escherichia coli. Biochem. Biophys. Res. Commun. 98:656-660.
14. Kawamura, S., K. Takamatsu, and K. Kitamura. 1992. Purification and characterization of S-modulin, a calcium-dependent regulator of cGMP phosphodiesterase in frog rod photoreceptors. Biochem. Biophys. Res. Commun. 186:411-417.
15. Kerson, G. W., J. A. Miernyk, and K. Budd. 1984. Evidence for the occurrence of and possible physiological role for cyanobacterial calmodulin. Plant Physiol. 75:222-227.
16. Klee, C. B., T. H. Crouch, and P. G. Richman. 1980. Calmodulin. Annu. Rev. Biochem. 69:489-515.
17. Klee, C. B., and T. C. Vanaman. 1982. Calmodulin. Adv. Protein Chem. 35:213-321.
18. Leppla, S. H. 1982. Anthrax toxin edema factor: a bacterial adenylate cyclase that increases cyclic AMP concentrations of eukaryotic cells. Proc. Natl. Acad. Sci. USA 79:3162-3166.
19. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275.
20. Manalan, A. S., and C. B. Klee. 1984. Calmodulin. Adv. Cyclic Nucleotide Protein Phosphorylation 18:227-278.
21. Means, A. R., M. F. VanBerkum, I. Bagchi, K. P. Lu, and C. D. Rasmussen. 1991. Regulatory functions of calmodulin. Pharmacol. Ther. 50:255-270.
22. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
23. Nagai, M., M. Endoh, H. Danbara, and Y. Nakase. 1994. Purification and characterization of Bordetella calmodulin-like protein. FEMS Microbiol. Lett. 116:169-174.
24. Pettersson, A., and B. Bergman. 1989. Calmodulin in heterocystous cyanobacteria: biochemical and immunological evidence. FEMS Microbiol. Lett. 51:95-99.
25. Ratnakar, P., and P. S. Murthy. 1992. Antitubercular activity of trifluoperazine, a calmodulin antagonist. FEMS Microbiol. Lett. 76:73-76.
26. Ratnakar, P., and P. S. Murthy. 1993. Trifluoperazine inhibits the incorporation of labelled precursors into lipids, proteins, and DNA of Mycobacterium tuberculosis H37Rv. FEMS Microbiol. Lett. 110:291-294.
27. Ratnakar, P., and P. S. Murthy. 1994. Preliminary studies on the inhibition of surface growth of isoniazid and streptomycin resistant strains of Mycobacterium tuberculosis H37Rv by trifluoperazine. Indian J. Tuberc. 41:35-37.
28. Reddy, P., and K. McKenney. 1996. Improved method for the production of M13 phage and single-stranded DNA for DNA sequencing. BioTechniques 20:854-859.
29. Reddy, P., A. Peterkofsky, and K. McKenney. 1989. Hyperexpression and purification of Escherichia coli adenylate cyclase using a vector designed for expression of lethal gene products. Nucleic Acids Res. 17:10473-10488.
30. Reddy, P. H., S. S. Burra, and P. S. Murthy. 1992. Correlation between calmodulin like protein, phospholipids, and growth in glucose-grown Mycobacterium phlei. Can. J. Microbiol. 38:339-342.
31. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
32. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
33. Sarma, P. V. G. K., P. U. Sarma, and P. S. Murthy. 1998. Isolation, purification and characterization of intracellular calmodulin like protein (CALP) from Mycobacterium phlei. FEMS Microbiol. Lett. 159:27-34.
34. Shyu, Y. T., and P. M. Foegeding. 1991. Purification and some characteristics of a calcium-binding protein from Bacillus cereus spores. J. Gen. Microbiol. 137:1619-1623.
35. Stone, K. L., and K. R. Williams. 1993. Enzymatic digestion of proteins and HPLC peptide isolation, p. 43-101. In P. Matsudaira (ed.), A practical guide to protein and peptide purification for microsequencing, 2nd ed. Academic Press, San Diego, Calif.
36. Swan, D. G., R. S. Hale, N. Dhillon, and P. F. Leadlay. 1987. A bacterial calcium-binding protein homologous to calmodulin. Nature 329:84-85.
37. Swan, D. G., J. Cortes, R. S. Hale, and P. F. Leadlay. 1989. Cloning, characterization, and heterologous expression of the Saccharopolyspora erythraea (Streptomyces erythraeus) gene encoding an EF-hand calcium-binding protein. J. Bacteriol. 171:5614-5619.
38. 2+-binding protein from rat brain. Biochem. Biophys. Res. Commun. 183:245-251.
39. 2+-dependent modulator protein (calmodulin) of bovine brain. J. Biol. Chem. 255:962-975.
40. Wolff, J., G. H. Cook, A. R. Goldhammer, and S. A. Berkowitz. 1980. Calmodulin activates prokaryotic adenylate cyclase. Proc. Natl. Acad. Sci. USA. 77:3841-3844.
41. Zahn, R., A. M. Buckle, S. Perrett, C. M. Johnson, F. J. Corrales, R. Golbik, and A. R. Fersht. 1996. Chaperone activity and structure of monomeric polypeptide binding domains of GroEL. Proc. Natl. Acad. Sci. USA 93: 15024-15029.
42. Zuber, M., T. A. Patterson, and D. L. Court. 1987. Analysis of nutR, a site required for transcription antitermination in phage lambda. Proc. Natl. Acad. Sci. USA 84:4514-4518.