Alcanivorax which prevails in oil-contaminated seawater exhibits broad substrate specificity for alkane degradation

Environmental Microbiology

Volumn 5, Issue 9, 2003, Pages 746-753

  Hara, Akihiro ^a,b   Syutsubo, Kazuaki ^a   Harayama, Shigeaki ^a  

^a MARINE BIOTECHNOLOGY INSTITUTE   (Japan)

^b DAINIPPON SUMITOMO PHARMA CO LTD   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ALKANES; BIODEGRADATION, ENVIRONMENTAL; CHROMATOGRAPHY, THIN LAYER; DNA, BACTERIAL; DNA, RIBOSOMAL; FLAME IONIZATION; GAMMAPROTEOBACTERIA; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; IN SITU HYBRIDIZATION, FLUORESCENCE; PETROLEUM; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SEAWATER; SEQUENCE ANALYSIS, DNA; SUBSTRATE SPECIFICITY; TERPENES; WATER MICROBIOLOGY; WATER POLLUTION, CHEMICAL;

ACINETOBACTER; ACINETOBACTER VENETIANUS; ALCANIVORAX; ALCANIVORAX BORKUMENSIS; BACTERIA (MICROORGANISMS); UNCULTURED MARINE BACTERIUM;

EID: 0041851188     PISSN: 14622912     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1468-2920.2003.00468.x     Document Type: Article

References (23)

1. Abraham, W.R., Meyer, H., and Yakimov, M. (1998) Novel glycine containing glucolipids from the alkane using bacterium Alcanivorax borkumensis. Biochem Biophys Acta 1393: 57-62.
2. Altschul, S.F., Gish, W., Miller, W., Myers, E.W., and Lipman, D.J. (1990) Basic local alignment search tool. J Mol Biol 215: 403-410.
3. Avigan, J., and Blumer, M. (1968) On the origin of pristane in marine organisms. J Lipid Res 9: 350-352.
4. Berekaa, M.M., and Steinbuchel, A. (2000) Microbial degradation of the multiply branched alkane 2,6,10,15,19,23-hexamethyltetracosane (squalane) by Mycobacterium fortuitum and Mycobacterium ratisbonense. Appl Environ Microbiol 66: 4462-4467.
5. Dutta, T.K., and Harayama, S. (2001) Biodegradation of n-alkylcycloalkanes and n-alkylbenzenes via new pathways in Alcanivorax sp. strain MBIC 4326. Appl Environ Microbiol 67: 1970-1974.
6. Goto, M., Kato, M., Asaumi, M., Shirai, K., and Venkateswaran, K. (1994) TLC/FID methods for evaluation of the crude-oil degrading capability of marine microorganisms. J Mar Biotech 2: 45-50.
7. Harayama, S., Kishira, H., Kasai, Y., and Shutsubo, K. (1999) Petroleum biodegradation in marine environments. J Mol Microbiol Biotechnol 1: 63-70.
8. Kasai, Y., Kishira, H., Syutsubo, K., and Harayama, S. (2001) Molecular detection of marine bacterial populations on beaches contaminated by the Nakhodka tanker oil-spill accident. Environ Microbiol 3: 246-255.
9. Kasai, Y., Kishira, H., Sasaki, T., Syutsubo, K., Watanabe, K., and Harayama, S. (2002) Predominant growth of Alcanivorax strains in oil-contaminated and nutrient-supplemented sea water. Environ Microbiol 4: 141-147.
10. Komukai-Nakamura, S., Sugiura, K., Yamauchi-Inomata, Y., Toki, H., Venkateswaran, K., Yamamoto, S., et al. (1996) Construction of bacterial consortia that degrade arabian light crude oil. J Ferment Bioeng 82: 570-574.
11. Maki, H., Sasaki, T., Sasaki, E., Ishihara, M., Goto, M., and Harayama, S. (1999) Use of wastewater sludge for the amendment of crude oil bioremediation in meso-scale beach simulating tanks. Environ Technol 20: 625-632.
12. Marmur, J. (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3: 208-218.
13. McKenna, E.J., and Kallio, R.E. (1971) Microbial metabolism of the isoprenoid alkane pristane. Proc Nat Acad Sci USA 68: 1552-1554.
14. Muyzer, G., de Waal, E.C., and Uitterlinden, A.G. (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59: 695-700.
15. Pirnik, M.P., Atlas, R.M., and Bartha, R. (1974) Hydrocarbon metabolism by Brevibacterium erythrogenes: normal and branched alkanes. J Bacteriol 119: 868-878.
16. Prince, R.C., Eimendorf, D.L., Lute, J.R., Hsu, C.S., Haith, C.E., Senius, J.D., et al. (1994) 17α, 21β (H)-hopane as a conserved internal marker for estimating the biodegradation of crude oil. Environ Sci Technol 28: 142-145.
17. Rossini, F.D. (1960) Hydrocarbons in petroleum. J Chem Educ 39: 554-561.
18. Rowe, R., Todd, R., and Waide, J. (1977) Microtechnique for most-probable-number analysis. Appl Environ Microbiol 33: 675-680.
19. Syutsubo, K., Kishira, H., and Harayama, S. (2001) Development of specific oligonucleotide probes for the identification and in situ detection of hydrocarbon-degrading Alcanivorax strains. Environ Microbiol 3: 371-379.
20. Wagner, M., Erhart, R., Manz, W., Amann, R., Lemmer, H., Wedi, D., and Schleifer, K.H. (1994) Development of an rRNA-targeted oligonucleotide probe specific for the genus Acinetobacter and its application for in situ monitoring in activated sludge. Appl Environ Microbiol 60: 792-800.
21. Wang, Z., Fingas, M., and Ken, L. (1994) Fractionation of a light crude oil and identification and quantitation of aliphatic, aromatic, and biomarker compounds by GC-FID and GC-MS, Part II. J Chromatogr Sci 32: 367-382.
22. Watanabe, K., Yamamoto, S., Hino, S., and Harayama, S. (1998) Population dynamics of phenol-degrading bacteria in activated sludge determined by gyrB-targeted quantitative PCR. Appl Environ Microbiol 64: 1203-1209.
23. Yakimov, M.M., Golyshin, P.N., Lang, S., Moore, E.R., Abraham, W.R., Lunsdorf, H., and Timmis, K.N. (1998) Alcanivorax borkumensis gen nov., sp. nov., a new, hydrocarbon-degrading and surfactant-producing marine bacterium. Int J Syst Bacteriol 48: 339-348.