Response to mitogens of chicken splenocytes determined by three methods of measurement

Animal Science Journal

Volumn 74, Issue 1, 2003, Pages 31-36

  Kondo, Yasuhiro ^a   Okimoto, Yohko ^a   Abe, Asaki ^a  

^a OKAYAMA UNIVERSITY   (Japan)

Author keywords

Chicken; Mitogen; MTT assay; Splenocyte

Indexed keywords

GALLUS GALLUS;

EID: 0041786627     PISSN: 13443941     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1344-3941.2003.00083.x     Document Type: Article

References (10)

1. Berridge MV, Tan AS. 1993. Characterization of the cellular reduction of 3-(4-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT): subcellular localization, subcellular dependence, and involvement of mitochondrial electron transport in MTT reduction. Archives of Biochemistry and Biophysics 303, 474-482.
2. Cahyaningsih U, Kondo Y, Abe A, Tanabe A. 1990. Presence of the diurnal rhythms of T cell counts and T cell activities in chicks. Japanese Poultry Science 27, 270-280.
3. Chen CH, Campbell PA, Newman LS. 1990. MTT colorimetric assay detects mitogen responses of spleen but not blood lymphocytes. International Archives of Allergy and Applied Immunology 93, 249-255.
4. Kondo Y, Aoki T, Abe A, Tanabe A. 1995. MTT (3- [4,5-dimethylthiazol-2-yl] -2, 5-diphenyltetrazolium bromide) colorimetric assay for mitogen-induced activation of chicken peripheral blood lymphocytes. Animal Science and Technology 66, 766-769.
5. Kondo Y, Kitano R, Abe A, Tanabe A. 1994. Ontogenetic study on alloreactivity in the chicken: analysis by graft versus host reaction and mixed lymphocyte reaction. Japanese Poultry Science 31, 9-19.
6. Lee LF. 1974. In vitro assay of mitogen stimulation of avian peripheral lymphocytes. Avian Disease 18, 602-609.
7. Lee LF. 1978. Chicken lymphocyte stimulation by mitogen: a microassay with whole-blood cultures. Avian Disease 22, 296-307.
8. Lu YS, Lapan RF. 1974. Splenic cell mitogenic responses in Marek's disease: comparison between noninfected tumor bearing and nontumor-bearing infected chicken. American Journal of Veterinary Research 35, 977-980.
9. Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65, 55-63.
10. Slater TF, Sawyer B, Straudi U. 1963. Studies on succinate-tetrazolium reductase system III. Biochimica et Biophysica Acta 77, 383-393.