Nanosecond dynamics of the mouse acetylcholinesterase Cys 69-Cys96 omega loop

Journal of Biological Chemistry

Volumn 278, Issue 33, 2003, Pages 30905-30911

  Shi, Jianxin ^a   Tai, Kaihsu ^a   McCammon, J Andrew ^a   Taylor, Palmer ^a   Johnson, David A ^b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANISOTROPY; CONFORMATIONS; ENZYME KINETICS; FLUORESCENCE; MOLECULAR BIOLOGY; MOLECULAR DYNAMICS; MOLECULAR STRUCTURE;

TIME-RESOLVED SPECTROSCOPY;

BIOCHEMICAL ENGINEERING;

5 [[[(2 ACETYL)AMINO]ETHYL]AMINO]NAPHTHALENE 1 SULFONIC ACID; ACETYLCHOLINESTERASE; AMINO ACID; CYSTEINE; FASCICULIN; SULFONIC ACID DERIVATIVE; UNCLASSIFIED DRUG;

ANISOTROPY; ARTICLE; CHOLINERGIC SYSTEM; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME PURIFICATION; FLUORESCENCE; GENE EXPRESSION; MOUSE; MUTAGENESIS; NEUROTRANSMISSION; NONHUMAN; PRIORITY JOURNAL; STEADY STATE; STOICHIOMETRY;

ACETYLCHOLINESTERASE; ANIMALS; BINDING SITES; CELL LINE; CYSTINE; FLUORESCENCE POLARIZATION; HUMANS; KIDNEY; LIGANDS; MICE; MUTAGENESIS; PROTEIN STRUCTURE, TERTIARY; TIME FACTORS;

EID: 0041731891     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M303730200     Document Type: Article

References (24)

1. Rosenberry, T. L. (1975) Adv. Enzymol. Relat. Areas Mol. Biol. 43, 103-218
2. Quinn, D. M. (1987) Chem. Rev. 87, 955-979
3. Sussman, J. L., Harel, M., Frolow, F., Oefner, C., Goldman, A., Toker, L., and Silman, I. (1991) Science 253, 872-879
4. Bourne, Y., Taylor, P., and Marchot, P. (1995) Cell 83, 503-512
5. Harel, M., Schalk, I., Ehretsabatier, L., Bouet, F., Goeldner, M., Hirth, C., Axelsen, P. H., Silman, I., and Sussman, J. L. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 9031-9035
6. Harel, M., Quinn, D. M., Nair, H. K., Silman, I., and Sussman, J. L. (1996) J. Am. Chem. Soc. 118, 2340-2346
7. Bourne, Y., Taylor, P., Bougis, P. E., and Marchot, P. (1999) J. Biol. Chem. 274, 2963-2970
8. Gilson, M. K., Straatsma, T. P., McCammon, J. A., Ripoll, D. R., Faerman, C. H., Axelsen, P. H., Silman, I., and Sussman, J. L. (1994) Science 263, 1276-1278
9. Wlodek, S. T., Clark, T. W., Scott, L. R., and McCammon, J. A. (1997) J. Am. Chem. Soc. 119, 9513-9522
10. Shen, T., Tai, K., Henchman, R. H., and McCammon, J. A. (2002) Acc. Chem. Res. 35, 332-340
11. Grochulski, P., Li, Y., Schrag, J. D., Bouthillier, F., Smith, P., Harrison, D., Rubin, B., and Cygler, M. (1993) J. Biol. Chem. 268, 12843-12847
12. Grochulski, P., Li, Y., Schrag, J. D., and Cygler, M. (1994) Protein Sci. 3, 82-91
13. Shi, J., Boyd, A. E., Radic, Z., and Taylor, P. (2001) J. Biol. Chem. 276, 42196-42204
14. Marchot, P., Ravelli, R. B., Raves, M. L., Bourne, Y., Vellom, D. C., Kanter, J., Camp, S., Sussman, J. L., and Taylor, P. (1996) Protein Sci. 5, 672-679
15. Ichiye, T., and Karplus, M. (1983) Biochemistry 22, 2884-2893
16. Tai, K., Shen, T., Borjesson, U., Philippopoulos, M., and McCammon, J. A. (2001) Biophys. J. 81, 715-724
17. Boyd, A. E., Marnett, A. B., Wong, L., and Taylor, P. (2000) J. Biol. Chem. 275, 22401-22408
18. Raves, M. L., Harel, M., Pang, Y. P., Silman, I., Kozikowski, A. P., and Sussman, J. L. (1997) Nat. Struct. Biol. 4, 57-63
19. Lakowicz, J. R. (1999) Principles of Fluorescence Spectroscopy, 2nd Ed., pp. 321-345, Kluwer Academic and Plenum Publishers, New York
20. Gangal, M., Cox, S., Lew, J., Clifford, T., Garrod, S. M., Aschbaher, M., Taylor, S. S., and Johnson, D. A. (1998) Biochemistry 37, 13728-13735
21. Gangal, M., Clifford, T., Deich, J., Cheng, X., Taylor, S. S., and Johnson, D. A. (1999) Proc. Natl. Acad. Sci. U. S. A. 96, 12394-12399
22. Kinosita, K., Jr., Kawato, S., and Ikegami, A. (1977) Biophys J. 20, 289-305
23. Steiner, R. F. (1991) in Topics in Fluorescence Spectroscopy (Lakowicz, J. R., ed) pp. 13-14, Plenum Press, New York
24. Zhou, H. X., Wlodek, S. T., and McCammon, J. A. (1998) Proc. Natl. Acad. Sci. U. S. A. 95, 9280-9283