Purification by monocional antibody affinity chromatography of virus-like particles associated with the '447' cytoplasmic male sterility of Vicia faba and investigation of their antigenic composition

Plant Science

Volumn 116, Issue 2, 1996, Pages 239-246

  Desvoyes, B ^a   Dulieu, P ^a  

^a UNIVERSITÉ DE FRANCHE COMTÉ   (France)

Author keywords

Affinity chromatography; Male sterility; Monoclonal antibodies; Vicia faba; VLPs

Indexed keywords

EID: 0041731188     PISSN: 01689452     EISSN: None     Source Type: Journal    
DOI: 10.1016/0168-9452(96)04390-7     Document Type: Article

References (13)

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8. E.V. Koonin, G.H. Choi, D.L. Nuss, R. Shapira and J.C. Carrington, Evidence for common ancestry of a chestnut blight hypovirulence-associated double-stranded RNA and a group of positive-strand RNA viruses. Proc. Natl. Acad. Sci. USA, 88 (1991) 10647-10651.
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10. G. Duc, La stérilité mâle nucléo-cytoplasmique 447 chez la féverole (Vicia faba L.). I. Etude du déterminisme maternel de l'instabilité phénotypique de la stérilité mâle. Agronomie, 4 (1984) 619-627.
11. P. Dulieu, F. Penin, H. Dulieu and D.C. Gautheron, Purification of virus-like particles from male sterile Vicia faba and detection by ELISA in crude leaf extracts. Plant Sci., 56 (1988) 9-14.
12. N. Berthaut, G. Duc, P. Dulieu, E. Boudon-Padieu and H. Dulieu, Application d'une méthode immunoenzymatique à la sélection pour la stabilité de la stérilité mâle cytoplasmique chez la féverole (Vicia faba L.). Agronomie, 11 (1991) 83-92.
13. P. Dulieu, Les particules cytoplasmiqucs associées à la stérilité mâle nucléo-cytoplasmique '447' de la féverole (Vicia faba L.): méthode de purification et mise au point d'un test immunoenzymatique de détection dans les plantes. Thesis No. 1088, University of Lyon I, France, 1988.