Exogenous DNA expression in eukaryotic cells following microinjection

Methods in Cell Science

Volumn 24, Issue 4, 2002, Pages 115-122

  Boyd, Ann ^a  

^a HOOD COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; GENE PRODUCT; GREEN FLUORESCENT PROTEIN; NUCLEIC ACID; PLASMID DNA;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL GROWTH; CELL STRAIN MCF 7; CHO CELL; CLINICAL PROTOCOL; CONTROLLED STUDY; CYTOPLASM; DNA SEQUENCE; EUKARYOTIC CELL; EXPERIMENTAL DESIGN; EXPRESSION VECTOR; FUSION GENE; GENE EXPRESSION; GENE TRANSFER; GENETIC CODE; IMMUNOHISTOCHEMISTRY; MICROINJECTION; NONHUMAN; PLASMID; PROMOTER REGION; RAT; SEQUENTIAL ANALYSIS;

ANIMALIA; EUKARYOTA; MINK CELL FOCUS-FORMING VIRUS; VECTORS;

EID: 0041664029     PISSN: 13815741     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1024401924835     Document Type: Article

References (30)

1. Diacumakos EG (1973). Methods for micromanipulation of human somatic cells in culture. Methods in Cell Biology 7: 287-311.
2. Capecchi MR (1980). High efficiency transformation by direct microinjection of DNA into cultured mammalian cells. Cell 22: 479-488.
3. Jaenisch R (1989). Transgenic animals. Science 240: 1468-1474.
4. Boyd AL, Samid D (1993). Review: Molecular biology of transgenic animals. J Anim Sci 71 (Suppl 3): 1-9.
5. Gordon JW, Ruddle FH (1985). DNA mediated genetic transformation of mouse embryos and bone marrow - A review. Gene 33: 121-136.
6. Woychik RP, Stewart TA, Davis LG, D'Eustachio P, Leder P (1985). An inherited limb deformity created by insertional mutagenesis in a transgenic mouse. Nature (London) 318: 36-40.
7. Pinkert CA, Widera G, Cowing C, Heber-Katz E, Palmiter RD, Flavell RA, Brinster RL (1985). Tissue specific, inducible and functional expression of the Ed MHC class II gene in transgenic mice. EMBO J 4: 2225-2230.
8. Boyd AL (1988). Gene transfer: A prelude to gene therapy. Methods in Cell Science 19: 231-242.
9. Boyd AL (1985). Expression of cloned genes microinjected into cultured mouse and human cells. Gene Anal Techn 2: 1-9.
10. Reeves R, Gorman C, Howard B (1985). Mini-chromosome assembly of nonintegrated plasmid DNA transfected into mammalian cells. Nucl Acids Res 13: 3599-3604.
11. Darnell JE (1982). Variety in the level of gene control in eukaryotic cells. Nature 297: 365-371.
12. Pabo CO, Sauer RT (1992). Transcription factors: Structural families and principles of DNA recognition. Ann Rev Biochem 61: 1053-1095.
13. Johnson PT, McKnight SL (1989). Eukaryotic transcriptional regulatory proteins. Ann Rev Biochem 58: 799-839.
14. Ptashne M (1988). How eukaryotic transcriptional activators work. Nature 335: 683-689.
15. Okayama H, Berg P (1982). High-efficiency cloning of full-length cDNA. Mol Cell Biol 2: 161-170.
16. Murray EJ (1991). Gene transfer and expression protocols. Methods in Mol Biol, Vol. 7 Clifton, NJ: Human Press.
17. Southern PJ, Berg P (1982). Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J Mol Appl Genet 1: 327-341.
18. Subramani S, DeLuca M (1988). Applications of the firefly luciferase as a reporter gene. Genetic Engineering 10: 75-89.
19. Nordeen K (1988). Luciferase reporter gene vectors for analysis of promoters and enhancers. BioTechniques 6:454-456.
20. Chalfie M, Tu Y, Euskirchem G, Ward WW, Prasher DC (1994). Green fluorescent protein as a marker for gene expression. Science 263: 802-805.
21. Kain SR, Adams M, Kondepudi A, Yang TT, Ward WW, Kitts P (1995). Green fluorescent protein as a reporter of gene expression and protein localization. BioTechniques 217: 650-655.
22. MacGregor CR, Caskey CT (1989). Construction of plasmids that express E. coli B-galaetosidase in mammalian cells. Nucl Acids Res 17: 2365-2374.
23. Berg P (1981). Dissections and reconstructions of genes and chromosomes. Science 213: 293-303.
24. Khoury G, Gruss P (1983). Enhancer elements. Cell 33: 313-314.
25. Laimins L, Khoury G, Gorman C, Howard B, Gruss P (1982). Host specific activation of transcription by tandem repeats from Simian Virus 40 and Moloney murine sarcoma virus. Proc Natl Acad Sci USA 79: 6453-6457.
26. Benoist C, Chambon P (1981). In vivo sequence requirements of the SV40 early promoter region. Nature 290: 304-310.
27. Serfling E, Jasin M, Schaffner W (1985). Enhancers and eukaryotic gene transcription. Trends in Genetics 1: 224-230.
28. Heim R, Cubitt AB, Tsien RY (1995). Improved green fluorescence. Nature 373: 663-664.
29. Levy JP, Muldoon RR, Zolotukhin S, Link CJ (1996). Retroviral transfer and expression of humanized, red-shifted green fluorescent protein gene into human tumor cells. Nature Biotech 14: 610-614.
30. White JA, Carter SG, Ozer HL, Boyd AL (1992). Cooperatively of SV40 T antigen and ras in progressive stages of transformation of human fibroblasts. Exp Cell Res 203: 157-163.