Interference of chlorophyll a in liquid scintillation counting of phytoplankton productivity samples by the 14C technique

Estuarine, Coastal and Shelf Science

Volumn 56, Issue 5-6, 2003, Pages 957-960

  Nayar, S ^a   Goh, B P L ^b   Chou, L M ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b NANYANG TECHNOLOGICAL UNIVERSITY   (Singapore)

Author keywords

14C tracer technique; Chlorophyll a; Errors in productivity measurements; Eutrophic waters; Peroxide addition; Phytoplankton pigments; Phytoplankton productivity; Pigment interference; Ponggol estuary; Scintillation counting

Indexed keywords

CHLOROPHYLL A; ERROR ANALYSIS; EUTROPHIC ENVIRONMENT; MEASUREMENT METHOD; PHYTOPLANKTON; PRODUCTIVITY;

EID: 0041625901     PISSN: 02727714     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0272-7714(02)00292-5     Document Type: Article

References (20)

1. 14 method of measuring primary productivity. Limnology and Oceanography 12, 121-124.
2. 14C primary production estimates. Limnology and Oceanography 7, 121-130.
3. Faust, M. A., & Norris, K. H. (1985). In-vivo spectrophotometric analysis of photosynthetic pigments in natural populations of phytoplankton. Limnology and Oceanography 30, 1316-1322.
4. Goldman, C. R., & Mason, D. T. (1962). Inorganic precipitation of carbon in productivity experiments utilizing carbon-14. Science 132, 1049-1050.
5. Hansen, D. L., & Bush, E. T. (1967). Improved solubilisation procedures for liquid scintillation counting of biological materials. Analytical Biochemistry 18, 320-332.
6. Holm-Hansen, O., Lorenzen, C. J., Holmes, R. W., & Strickland, J. D. (1966). Flurometric determination of chlorophyll. Journal du Conseil Permanent International pour l' Exploration de la Mer 30, 3-15.
7. Konovalov, B. V., & Bekasova, O. D. (1969). Method for determining the pigment content of marine phytoplankton without extraction. Okeanology 9, 717-725.
8. 14C method of primary production measurements. Limnology and Oceanography 24, 917-928.
9. Lorenzen, C. J. (1966). A method for the continuous measurement of in-vivo chlorophyll concentration. Deep Sea Research 13, 223-227.
10. 14C method of measuring primary production. Limnology and Oceanography 18, 319-324.
11. 14C method of primary production measurement. Journal of Phycology 8, 37-43.
12. Parsons, T. R., Maita, Y., & Lalli, C. M. (1989). A manual of chemical and biological methods for sea-water analysis (173 pp.). New York: Pergamon Press.
13. Pugh, P. R. (1973). An evaluation of liquid scintillation counting techniques for use in aquatic primary production studies. Limnology and Oceanography 18, 310-319.
14. Richards, F. A., & Thompson, T. G. (1952). The estimation and characterization of plankton populations by pigment analysis: II. A spectrophotometric method for the estimation of plankton pigments. Journal of Marine Research 11, 156-172.
15. 14) for measuring organic production in the sea. Journal du Conseil Permanent International pour l' Exploration de la Mer 18, 117-140.
16. Venrick, E. L. (1987). On the fluorometric determinations of filter-retained pigments. Limnology and Oceanography 32, 492-493.
17. 14C-labelled phytoplankton on membrane filters. Journal of the Fisheries Research Board of Canada 25, 2219-2224.
18. Wetzel, R. G., & Likens, G. E. (2000). Limnological analysis (429 pp.). New York: Springer.
19. Williams, P. J. L., Berman, T., & Holm-Hanson, O. (1972). Potential sources of error in the measurement of low rates of planktonic photosynthesis and excretion. Nature 236, 91-92.
20. Yentsch, C. S. (1957). A non-extractable method for the quantitative estimation of chlorophyll in algal cultures. Nature 179, 1302-1304.