Efficiency and cost effectiveness: PAGE-SSCP versus MDE and Phast gels for the identification of unknown β thalassaemia mutations

Journal of Clinical Pathology - Molecular Pathology

Volumn 56, Issue 4, 2003, Pages 237-239

  Gupta, A ^a   Agarwal, S ^a  

^a SANJAY GANDHI POSTGRADUATE INSTITUTE OF MEDICAL SCIENCES   (India)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GLOBIN;

ARTICLE; BETA THALASSEMIA; CONTROLLED STUDY; GENE IDENTIFICATION; GENE MUTATION; GENETIC ANALYSIS; GENETIC SCREENING; HUMAN; INTERMETHOD COMPARISON; OUTCOMES RESEARCH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SINGLE STRAND CONFORMATION POLYMORPHISM;

AUTORADIOGRAPHY; BETA-THALASSEMIA; COST-BENEFIT ANALYSIS; COSTS AND COST ANALYSIS; DNA MUTATIONAL ANALYSIS; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GELS; GLOBINS; HUMANS; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; SENSITIVITY AND SPECIFICITY;

EID: 0041565464     PISSN: 13668714     EISSN: None     Source Type: Journal    
DOI: 10.1136/mp.56.4.237     Document Type: Article

References (5)

1. Varawalla NY, Old JM, Sarkar R, et al. The spectrum of β-thalassemia mutations on the Indian subcontinent: the basis for prenatal diagnosis. Br J Haematol 1991;78:242-24.
2. Langemeier JL, Cook RF, Issel CJ, et al. Comparative sensitivity of alternative single strand conformation polymorphism (SSCP) methods. Biotechniques 1994;17:490-6.
3. Humphries SE, Gudnason V, Whitall, et al. Single-strand conformation polymorphism analysis with high throughput modifications, and its use in mutation detection in familial hypercholestrolemia. Clin Chem 1997;43:427-35.
4. Sentinelli F, Lovari S, Vitale M, et al. A simple method for non-radioactive PCR-SSCP using MDE gel solution and a midi gel format: application for the detection of variants in the GLUT1 and CTLA-4 genes. J Biotechnol 2000;78:201-4.
5. Mohabeer AJ, Hiti AL, Martin WJ. Non-radioactive single strand conformation polymorphism (SSCP) using the Pharmacia "Phast system". Nucleic Acids Res 1991;19:3154.