Immunogenicity of Sm32 synthetic peptides derived from the Schistosoma mansoni adult worm

Immunology Letters

Volumn 88, Issue 3, 2003, Pages 211-219

  Noya, Oscar ^a   Alarcón de Noya, Belkisyolé ^a   Guzmán, Fanny ^b   Bermúdez, Henry ^a  

^a UNIVERSIDAD CENTRAL DE VENEZUELA   (Venezuela)

^b FUNDACIÓN INSTITUTO DE INMUNOLOGÍA DE COLOMBIA FIDIC   (Colombia)

Author keywords

Asparagynil endoprotease; Hemoglobinase; Schistosoma mansoni; Sm32; Synthetic peptides

Indexed keywords

LEGUMAIN; PEPTIDE; PROTEINASE; SM 32; UNCLASSIFIED DRUG;

ANTIBODY PRODUCTION; ANTIGEN DETECTION; ANTIGEN RECOGNITION; ARTICLE; IMMUNIZATION; IMMUNOGENICITY; MOLECULAR RECOGNITION; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITOSIS; PRIORITY JOURNAL; RABBIT; SCHISTOSOMA MANSONI; SERODIAGNOSIS; WESTERN BLOTTING;

EID: 0041519122     PISSN: 01652478     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-2478(03)00086-5     Document Type: Article

References (25)

1. Agrew A.M., Fulford A.J., De Jonge N., Krijger F.W., Rodriguez-Chacón M., Gursmann V., Deelder A.M. The relationship between, worm burdens and levels of a circulating antigen (CAA) of five species of Schistosoma in mice. Parasitology. 111:1995;67-76.
2. Noya O., Fermín Z., Alarcón de Noya B., Losada S., Colmenares C., Hermoso T. Humoral immune response of children with chronic schistosomiasis. Isotype recognition of adult worm antigens. Parasite Immunol. 17:1995;319-328.
3. Ruppel A., Diesfeld H.F., Rother U. Immunoblot analysis of Schistosoma mansoni antigens with sera of schistosomiasis patients: diagnostic potential of an adult schistosome polypeptide. Clin. Exp. Immunol. 62:1985;499-506.
4. Klinkert M.Q., Felleisen R., Link G., Ruppel A., Beck E. Primary structures of Sm 31/32 diagnostic proteins of Schistosoma mansoni and their identification as proteases. Mol. Biochem. Parasitol. 33:1989;113-122.
5. Davis A.H., Nanduri J., Watson D.C. Cloning and gene expression of Schistosoma mansoni protease. J. Biol. Chem. 262:1987;12851-12855.
6. Takeda O., Miura Y., Mitta M., Matsushita H., Kato I., Abe Y., Yokosawa H., Ishii S. Isolation and analysis of cDNA encoding a precursor of Canavalia ensiformis asparaginyl endopeptidase (legumain). J. Biochem. 16:1994;541-546.
7. Gotz B., Klinkert M.Q. Expression and partial characterization of a cathepsin B-like enzyme (Sm-31) and a proposed "hemoglobinase" (Sm-32) Schistosoma mansoni. Biochem. J. 290:1993;801-806.
8. Dalton J.P., Smith A.M., Clongh K.A., Brindley P.J. Digestion of hemoglobin by Schistosomas: 35 years on. Parasitol. Today. 11:1995;299-303.
9. Dalton J.P., Brindley P.J. Schistosome asparaginil endopeptidase Sm 32 in hemoglobin digestion (Letter). Parasitol. Today. 12:1996;125.
10. Li Y.L., Idris M.A., Corachan M., Han J.J., Kerschfink M., Ruppel A. Circulating antigens in schistosomiasis: detection of 31/32 kDa proteins in sera from patients infected with Schistosoma japonicum, S. mansoni, S. haematobium or S. intercalatum. Parasitol. Res. 82:1996;14-18.
11. Smithers S.R., Terry R.J. The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of the adult worm. Parasitology. 55:1965;695-700.
12. Lowry O.H., Rossenbrough N.J., Farr A.L., Randall R.J. Protein measurements with the Folin reagent. J. Biol. Chem. 193:1951;265-275.
13. Merrifield R.B. Solid phase peptide synthesis: the synthesis of a tetrapeptide. J. Am. Chem. Soc. 85:1963;2149-2154.
14. Houghten R., De Graw S., Hoffmann B.M., Frizzel S. Simultaneous multiple peptide synthesis: the rapid preparation of large numbers of discrete peptides for biological, immunological, and methodological studies. Biotechniques. 6:1986;522-528.
15. Tam J.P., Heath W.F., Merrifield R.B. Sn2 deprotection of synthetic peptides with a low concentration of HF in dimethyl sulfide: evidence and application in peptide synthesis. J. Am. Chem. Soc. 105:1983;6242-6455.
16. Noya O., Alarcón de Noya B. The multiple antigen blot assay (MABA): a simple immunoenzymatic technique for simultaneous screening of multiple antigens. Immunol. Lett. 63:1998;53-56.
17. Hopp T.P., Woods K.R. Prediction of protein antigen determinants from amino acid sequences. Proc. Natl. Acad. Sci. USA. 78:1981;3824-3828.
18. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-684.
19. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Procedure and some applications. Proc. Natl. Acad. Sci. USA. 76:1979;4350-4354.
20. Patarroyo M.E., Amador R., Clavijo P., Moreno A., Guzman F., Romero P., Tascon R., Franco A., Murillo L.A., Ponton G., Trujillo G. A synthetic vaccine protects humans against challenge with asexual blood stages of Plasmodium falciparum malaria. Nature. 332:1988;158-161.
21. Noya O., Alarcón de Noya B., Ballen D., Zerpa N., Colmenares C., Losada S., Bermúdez H. Use of synthetic peptides derived from adult worm proteins of Schistosoma mansoni, in the diagnosis of schistosomiasis. Mem. I. Oswaldo Cruz. 93:1998;157-158.
22. Devesa M., de Saez A., León G., Sirit F., Cosson C., Bermúdez H., Liprandi F., Noya O., Pujol F.H. Restricted isotypic antibody reactivity to hepatitis C virus synthetic peptides in immunocompromised patients. Clin. Diagn. Lab. Immunol. 6:1999;279-281.
23. Klinkert M.Q., Boomer K., Moser D., Felleisen R., Link G., Ruppel A., Beck E. Immunological analysis of cloned Schistosoma mansoni antigens Sm31 and Sm32 with sera of schistosomiasis patients. Trop. Med. Parasitol. 42:1991;319-324.
24. Idris M.A., Ruppel A. Diagnostic Mr 31/32. Schistosoma mansoni proteins (Sm 31/32) reaction with sera from Sudanese patients infected with Schistosoma mansoni or Schistosoma haematobium. J. Helminthol. 62:1990;95-101.
25. El Meanaway M.A., Aji T., Phillips N.F., Davis R.E., Salata R.A., Malhotra I., McClain D., Aikawa M., Davis A.H. Definition of the complete Schistosoma mansoni hemoglobinase mRNA sequence and gene expression in developing parasites. Am. J. Trop. Med. Hyg. 43:1990;67-78.