Antibody phenotyping test for the human apolipoprotein E2 isoform

Clinical Chemistry

Volumn 49, Issue 9, 2003, Pages 1524-1526

  Raffaï, Robert L ^b   McPherson, Ruth ^a   Weisgraber, Karl H ^b   Innerarity, Thomas L ^b   Rassart, Eric ^c   Bersot, Thomas P ^b   Milne, Ross W ^a  

^a UNIVERSITY OF OTTAWA HEART INSTITUTE   (Canada)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITÉ DU QUÉBEC À MONTRÉAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; APOLIPOPROTEIN E2; MONOCLONAL ANTIBODY;

ALZHEIMER DISEASE; ANTIBODY SPECIFICITY; ARTICLE; ATHEROSCLEROSIS; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; PROTEIN ANALYSIS;

ANTIBODIES, MONOCLONAL; APOLIPOPROTEIN E2; APOLIPOPROTEINS E; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENOTYPE; HUMANS; PHENOTYPE; PROTEIN ISOFORMS;

EID: 0041441089     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/49.9.1524     Document Type: Article

References (7)

1. Bouthillier D, Sing CF, Davignon J. Apolipoprotein E phenotyping with a single gel method: application to the study of informative matings. J Lipid Res 1983;24:1060-9.
2. Hixson JE, Vernier DT. Restriction isotyping of human apolipoprotein E by gene amplification and cleavage with Hhal. J Lipid Res 1990;31:545-8.
3. Milne RW, Douste-Blazy P, Marcel YL, Retegui L. Characterization of monoclonal antibodies against human apolipoprotein E. J Clin Invest 1981;68:111-7.
4. Weisgraber KH, Rall SC Jr, Mahley RW, Milne RW, Marcel YL, Sparrow JT. Human apolipoprotein E. Determination of the heparin binding sites of apolipoprotein E3. J Biol Chem 1986;261:2068-76.
5. Raffaï R, Maurice R, Weisgraber K, Inneranity T, Wang X, MacKenzie R, et al. Molecular characterization of two monoclonal antibodies specific for the LDL receptor-binding site of human apolipoprotein E. J Lipid Res 1995;36:1905-18.
6. Uchida Y, Ito S, Nukina N. Sandwich ELISA for the measurement of Apo-E4 levels in serum and the estimation of the allelic status of Apo-E4 isoforms. J Clin Lab Anal 2000;14:260-4.
7. Weisgraber KH, Mahley RW. Human apolipoprotein E: the Alzheimer's dis-ease connection. FASEB J 1996;10:1485-94.