Mass spectrometric analysis of the reactions catalyzed by L-2-haloacid dehalogenase mutants and implications for the roles of the catalytic amino acid residues

Journal of Molecular Catalysis B: Enzymatic

Volumn 23, Issue 2-6, 2003, Pages 337-345

  Li, Yong Fu ^a,c   Kurihara, Tatsuo ^a   Ichiyama, Susumu ^a,d   Miyagi, Masaru ^b,e   Tsunasawa, Susumu ^b,f   Esaki, Nobuyoshi ^a  

^a KYOTO UNIVERSITY   (Japan)

^b TAKARA SHUZO CO LTD   (Japan)

^c NATIONAL CANCER INSTITUTE   (United States)

^d GAKUSHUIN UNIVERSITY   (Japan)

^e UNIVERSITY OF NORTH DAKOTA SCHOOL OF MEDICINE AND HEALTH SCIENCES   (United States)

^f SHIMADZU CORPORATION   (Japan)

Author keywords

L 2 Haloacid dehalogenase; Mass spectrometry; Reaction mechanism

Indexed keywords

AMINO ACIDS; CATALYSIS; ESTERS; MASS SPECTROMETRY; SUBSTRATES;

MOLECULAR MASSES;

ENZYME KINETICS;

AMINO ACID; BACTERIAL ENZYME; DEHALOGENASE; ESTER; MUTANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; ENZYME ACTIVITY; ENZYME SUBSTRATE; HYDROLYSIS; MASS SPECTROMETRY; MOLECULAR WEIGHT; PSEUDOMONAS; REACTION ANALYSIS;

PSEUDOMONAS; PSEUDOMONAS SP.; PSEUDOMONAS SP. YL;

EID: 0041431072     PISSN: 13811177     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1381-1177(03)00097-3     Document Type: Article

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