Improvement in thermostability and psychrophilicity of psychrophilic alanine racemase by site-directed mutagenesis

Journal of Molecular Catalysis B: Enzymatic

Volumn 23, Issue 2-6, 2003, Pages 389-395

  Yokoigawa, Kumio ^a   Okubo, Yoko ^a   Soda, Kenji ^b   Misono, Haruo ^c  

^a NARA WOMEN'S UNIVERSITY   (Japan)

^b KANSAI UNIVERSITY   (Japan)

^c KOCHI UNIVERSITY   (Japan)

Author keywords

Alanine racemase; Bacillus psychrosaccharolyticus; Psychrophilicity; Site directed mutagenesis; Thermostability

Indexed keywords

AMINO ACIDS; CATALYST ACTIVITY; MUTAGENESIS;

ACTIVE SITES;

ENZYME KINETICS;

ALANINE; ALANINE RACEMASE; ARGININE; GLUTAMIC ACID; PYRIDOXAL 5 PHOSPHATE; VALINE;

ARTICLE; CIRCULAR DICHROISM; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME STABILITY; GEOBACILLUS STEAROTHERMOPHILUS; HYDROPHILICITY; NONHUMAN; SITE DIRECTED MUTAGENESIS; TEMPERATURE SENSITIVITY; THERMOSTABILITY;

BACILLUS PSYCHROSACCHAROLYTICUS; GEOBACILLUS STEAROTHERMOPHILUS;

EID: 0041431061     PISSN: 13811177     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1381-1177(03)00103-6     Document Type: Article

References (41)

1. Pennisi E. Science. 276:1997;705.
2. Aghajari N., Feller G., Gerday C., Haser R. Protein Sci. 5:1996;2128.
3. Aghajari N., Feller G., Gerday C., Haser R. Protein Sci. 7:1998;564.
4. Bentahir M., Feller G., Aittaleb M., Lamotte-Brasseur J., Himri T., Chessa J.P., Gerday C. J. Biol. Chem. 275:2000;11147.
5. Chessa J.P., Petrescu I., Bentahir M., Van Beeumen J., Gerday C. Biochim. Biophys. Acta. 1479:2000;265.
6. Davail S., Feller G., Narinx E., Gerday C. J. Biol. Chem. 269:1994;17448.
7. Feller G., Gerday C. Cell Mol. Life Sci. 53:1997;830.
8. Kim S.Y., Hwang K.Y., Kim S.H., Sung H.C., Han Y.S., Cho Y. J. Biol. Chem. 274:1999;11761.
9. Schroder Leiros H.K., Willassen N.P., Smalas A.O. Eur. J. Biochem. 267:2000;1039.
10. Villeret V., Chessa J.P., Gerday C., Van Beeumen J. Protein Sci. 6:1997;2462.
11. Cheng Y.-Q., Walton J.D. J. Biol. Chem. 275:2000;4906.
12. Uo T., Yoshimura T., Tanaka N., Takegawa K., Esaki N. J. Bacteriol. 183:2001;2226.
13. Nomura T., Yamamoto I., Morishita F., Furukawa Y., Matsushima O. J. Exp. Zool. 289:2001;1.
14. Shibata K., Shirasuna K., Motegi K., Kera Y., Abe H., Yamada R. Comp. Biochem. Physiol. B. 126:2000;599.
15. N. Esaki, T. Kurihara, K. Soda, in: K. Drauz, H. Waldmann (Eds.), Enzyme Catalysis in Organic Synthesis, vol. 3, second ed., Wiley-VCH, Weinheim, 2002, p. 1281.
16. Esaki N., Walsh C.T. Biochemistry. 25:1986;3261.
17. Inagaki K., Tanizawa K., Badet B., Walsh C.T., Tanaka H., Soda K. Biochemistry. 25:1986;3268.
18. Strych U., Huang H.C., Krause K.L., Benedik M.J. Curr. Microbiol. 41:2000;290.
19. Strych U., Penland R.L., Jimenez M., Krause K.L., Benedik M.J. FEMS Microbiol. Lett. 196:2000;93.
20. Wasserman S.A., Daub E., Grisafi P., Botstein D., Walsh C.T. Biochemistry. 23:1984;5182.
21. Ferrari E., Henner D.J., Yang M.Y. Biotechnology. 3:1985;1003.
22. Galakatos N.G., Daub E., Botstein D., Walsh C.T. Biochemistry. 25:1986;3255.
23. Hols P., Defrenne C., Ferain T., Derzelle S., Delplace B., Delcour J. J. Bacteriol. 179:1997;3804.
24. Lobocka M., Hennig J., Wild J., Klopotowski T. J. Bacteriol. 176:1994;1500.
25. Shaw J.P., Petsko G.A., Ringe D. Biochemistry. 36:1997;1329.
26. Tanizawa K., Ohshima A., Scheidegger A., Inagaki K., Tanaka H., Soda K. Biochemistry. 27:1988;1311.
27. Yokoigawa K., Hirasawa R., Ueno H., Okubo Y., Umesako S., Soda K. Biochem. Biophys. Res. Commun. 288:2001;676.
28. Okubo Y., Tomioka R., Yokoigawa K., Kawai H. J. Home Econ. Jpn. 46:1995;1135.
29. Okubo Y., Yokoigawa K., Kawai H. J. Biosci. Bioeng. 87:1999;241.
30. Yokoigawa K., Kawai H., Endo K., Lim Y.H., Esaki N., Soda K. Biosci. Biotech. Biochem. 57:1993;93.
31. Yokoigawa K., Okubo Y., Kawai H., Esaki N., Soda K. J. Mol. Cat. B Enzymatic. 12:2001;27.
32. Okubo Y., Yokoigawa K., Esaki N., Soda K., Kawai H. Biochem. Biophys. Res. Commun. 256:1999;333.
33. Okubo Y., Yokoigawa K., Esaki N., Soda K., Misono H. FEMS Microbiol. Lett. 192:2000;169.
34. M.L. Tutino, L. Birolo, B. Fontannella, K. Mainolfi, F. Vinci, G. Sannia, G. Marino, in: R. Margesin, F. Schinner (Eds.), Cold-Adapted Organisms, Springer, Berlin, Germany, 1999, p. 305.
35. Soda K. Anal. Biochem. 25:1968;228.
36. Adams E. Methods Enzymol. 62:1979;407.
37. C. Gerday, M. Aittaleb, J.L. Arpigny, E. Baise. J.P. Chessa, J.M. Francois, G. Garsoux, I. Petrescu, G. Feller, in: R. Margesin, F. Schinner (Eds.), Cold-Adapted Organisms, Springer, Berlin, Germany, 1999, p. 257.
38. Lonhienne T., Gerday C., Feller G. Biochim. Biophys. Acta. 1543:2000;1.
39. Almo S.C., Smith D.L., Danishefsky A.T., Ringe D. Protein Eng. 7:1994;405.
40. Tai C.H., Cook P.F. O-acetylserine sulfhydrylase. Adv. Enzymol. Relat. Areas Mol. Biol. 74:2000;185.
41. Yamanaka Y., Kazuoka T., Yoshida M., Yamanaka K., Oikawa T., Soda K. Biochem. Biophys. Res. Comm. 298:2002;632.