Application of thiopropyl sepharose 6B for removal of PCR inhibitors from DNA extracts of a thigh bone recovered from the sea

International Journal of Legal Medicine

Volumn 117, Issue 4, 2003, Pages 245-247

  Sørensen, Erik ^a   Hansen, Steen Holger ^a   Eriksen, Birthe ^a   Morling, Niels ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Forensic science; Removal of PCR inhibitors; Thiopropyl sepharose 6B

Indexed keywords

DNA; ENZYME INHIBITOR; SEA WATER; SEPHAROSE; TAQ POLYMERASE; THIOPROPYL SEPHAROSE 6B; UNCLASSIFIED DRUG;

ARTICLE; DECOMPOSITION; DNA EXTRACTION; FEMUR; FORENSIC IDENTIFICATION; FORENSIC SCIENCE; HUMAN; HUMAN TISSUE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEA;

DNA; DROWNING; FEMUR; FORENSIC SCIENCES; HUMANS; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION; SEPHAROSE;

EID: 0041427708     PISSN: 09379827     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00414-003-0375-6     Document Type: Article

References (9)

1. Williamson JM, Waye JS, Newall P, Bing DH, Blake E (1996) The use of a comprehensive approach for neutralization of PCR inhibitors found in forensic samples and its use in a homicide/sexual assault case. Proceedings from the Sixth International Symposium on Human Identification 1995. Promega Corporation, Madison, WI, pp 186-187
2. Shutler G, Gagnon P, Verret G, Kalyn H, Korkosh S, Johnston E, Halvorson J (1999) Removal of a PCR inhibitor and resolution of DNA STR types in mixed human-canine stains from a five year old case. J Forensic Sci 44:623-626
3. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor NY, pp E.3-E.4
4. Waye JS, Michaud D, Bowen JH, Fourney RM (1991). Sensitive and specific quantification of human genomic deoxyribonucleic acid (DNA) in forensic science specimens: casework examples. J Forensic Sci 36:1198-1203
5. Fridez F, Coquoz R (1996) PCR DNA typing of stamps: evaluation of the DNA extraction. Forensic Sci Int 78:103-110
6. Crainic K, Paraire F, Leterreux M, Durignon M, Mazancourt P de (2002). Skeletal remains presumed submerged in water for three years identified using PCR-STR analysis. J Forensic Sci 47:1025-1027
7. Walsh PS, Metzger DA, Higuchi R (1991) Chelex 100 as a Medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10:506-513
8. Wiegand P, Kleiber M (2001). Less is more - length reduction of STR amplicons using redesigned primer. Int J Legal Med 114:285-287
9. Parson W, Niederstatter H, Kochl S, Steinlechner M, Berger B (2001). When autosomal short tandem repeats fail: optimized primer and reaction design for Y-chromosome short tandem repeat analysis in forensic casework. Croat Med J 42:285-287