Use of endogenous host plasmids for generation of Escherichia coli O157:H7 and Shigella sonnei strains that stably express the green fluorescent protein

Plasmid

Volumn 50, Issue 2, 2003, Pages 161-167

  Monday, Steven R ^a   Weagant, Stephen D ^b   Feng, Peter ^a  

^a CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

^b FDA Pacific Regional Laboratory Northwest   (United States)

Author keywords

Control strains; GFP; Plasmids; Transposon insertion

Indexed keywords

ANTIBIOTIC AGENT; GREEN FLUORESCENT PROTEIN; PLASMID VECTOR;

ARTICLE; BACTERIAL CELL; BACTERIAL STRAIN; CELL FUNCTION; CONTROLLED STUDY; ESCHERICHIA COLI O157; EXPRESSION VECTOR; GENE LOSS; GENETIC MANIPULATION; GENETIC STABILITY; HOST CELL; NONHUMAN; PHENOTYPE; PROTEIN EXPRESSION; SHIGELLA SONNEI;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; INSERTION SEQUENCES; NEGIBACTERIA; SHIGELLA; SHIGELLA SONNEI; TRANSPOSONS; VECTORS;

EID: 0041426358     PISSN: 0147619X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0147-619X(03)00057-X     Document Type: Article

References (21)

1. Andersen J.B., Sternberg C., Poulsen L.K., Bjorn S.P., Molin S. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64:1998;2240-2246.
2. Andrews, W.A., June, G.A., Sherrod, P.S., 1998. Shigella. FDA Bacteriological Analytical Manual, eight ed. (rev.A), Assoc. Official Anal. Chem. International, Gaithersburg, MD, pp. 6.01-6.06.
3. Bazaral M., Helinski D.R. Circular DNA forms of colicinogenic factors E1, E2, and E3 from Escherichia coli. J. Mol. Biol. 36:1968;185-194.
4. Chalfie M., Tu Y., Euskierchen G., Ward W.W., Prasher D.C. Green fluorescent protein as a marker for gene expression. Science. 263:1994;802-805.
5. Cubitt A.B., Heim R., Adams S.R., Boyd A.E., Gross L.A., Tsien R.Y. Understanding, improving and using green fluorescent proteins. Trends Biochem. Sci. 20:1995;448-455.
6. Errampalli D., Leung K., Cassidy M.B., Kostrzynska M., Blears M., Lee H., Trevors J. Application of the green fluorescent protein as a molecular marker in environmental microorganisms. J. Microbiol. Methods. 35:1999;187-199.
7. Feng P., Monday S.R. Multiplex PCR for detection of trait and virulence factors in enterohemorrhagic Escherichia coli serotypes. Mol. Cell. Probes. 14:2000;333-337.
8. Fratamico P.M., Deng M.Y., Strobaugh T.P., Palumbo S.A. Construction and characterization of Escherichia coli O157:H7 strains expressing firefly luciferase and green fluorescent protein and their use in survival studies. J. Food Protect. 60:1997;1167-1173.
9. Hill W.E., Carlisle C.L. Loss of plasmids during enrichment for Escherichia coli. Appl. Environ. Microbiol. 41:1981;1046-1048.
10. Hitchins, A.D., Feng, P., Watkins, W.D., Rippey, S.R., Chandler, L.A., 1998. Escherichia coli and the coliform Bacteria. FDA Bacteriological Analytical Manual, eight ed. (rev.A), Assoc. Official Analy. Chem. International, Gaithersburg, MD. pp. 4.01-4.29.
11. Jamieson A.F., Bremmer D.A., Berquist P.L., Lane H.E. Characterization of plasmids from antibiotic-resistance Shigella isolated by agarose gels electrophoresis. J. Gen. Microbiol. 113:1979;73-81.
12. Levine M.M., Xu J., Kaper J.B., Lior H., Prado V., Tall B., Nataro J., Karch H., Wachsmuth I.K. A DNA probe to identify enterohemorrhagic Escherichia coli of O157:H7 and other serotypes that cause hemorrhagic colitis and hemolytic uremic syndrome. J. Infect. Dis. 156:1987;175-182.
13. Lorang J.M., Tuori R.P., Martinez J.P., Sawyer T.L., Redman R.S., Rollins J.A., Wolpert T.J., Johnson K.B., Rodriguez R.J., Dickman M.B., Ciuffetti L.M. Green fluorescent protein is lighting up fungal biology. Appl. Environ. Microbiol. 67:2001;1987-1994.
14. Menard R., Sansonetti P.J. Shigella flexneri: isolation of non-invasive mutants of Gram-negative pathogens. Methods Enzymol. 236:1994;493-509.
15. Monday S.R., Whittam T.S., Feng P. Genetic and evolutionary analysis of insertions in the gusA gene, which caused the absence of glucuronidase activity in Escherichia coli O157:H7. J. Infect. Dis. 184:2001;918-921.
16. Prasher D.C. Primary structure of Aequorea victoria green-fluorescent protein. Gene. 111:1992;229-233.
17. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual. second ed. 1989;Cold Springs Harbor Press, Cold Springs Harbor, NY.
18. Schmidt H., Karch H., Beutin L. The large-sized plasmids of enterohemorrhagic Escherichia coli O157 strains encode hemolysins which are presumably members of the E. coli α-hemolysin familiy. FEMS Microbiol. Lett. 117:1994;189-196.
19. Summers D. Timing, self-control and sense of direction are the secrets of multicopy plasmid stability. Mol. Microbiol. 29:1998;1137-1145.
20. Valdivia R.H., Hromockyj A.E., Monack D., Ramakrishnan L., Falkow S. Applications for green fluorescent protein (GFP) in the study of host-pathogen interactions. Gene. 173:1996;47-52.
21. Weagant S.D., Bryant J.L., Jinneman K.G. An improved rapid technique for isolation of Escherichia coli O157:H7 from foods. J. Food Protect. 58:1995;7-12.