Protoplast isolation and culture in sugar beet (Beta vulgaris L.)

Plant Cell Biotechnology and Molecular Biology

Volumn 3, Issue 1-2, 2002, Pages 11-20

  Gürel, Ekrem ^a   Gürel, Songül ^b   Kaya, Zeki ^c  

^a ABANT IZZET BAYSAL UNIVERSITY   (Turkey)

^b Sugar Institute   (Turkey)

^c MIDDLE EAST TECHNICAL UNIVERSITY   (Turkey)

Author keywords

Micro colony; Protoplast isolation; Sugar beet; Suspension culture

Indexed keywords

BETA VULGARIS; BETA VULGARIS SUBSP. VULGARIS;

EID: 0041425210     PISSN: 09722025     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Arcioni, S, Mariotti, D. and Pezzotti, M. 1985. Hedysarum coronarium L. In vitro conditions for plant regeneration from protoplasts and callus of various explants. J. Plant Physiol. 121: 141-148.
2. Babaoglu, M. 2000. Protoplast isolation in lupin (Lupinus mutabilis Sweet.): Determination of optimum explant sources and isolation conditions. Turkish J. Bot. 24: 177-186.
3. Bhat, S.R, Ford-Lyod, B.V. and Callow, J.A. 1985. Isolation of protoplasts and regeneration of callus from suspension cultures of cultivated beets. Plant Cell Rep. 4: 348-350.
4. Bhojwani, S.S. and Razdan, M.K. 1990. Plant Tissue Culture: Theory and Practice, Elsevier Science Publishers B.V., Amsterdam.
5. Blackhall, N.W., Davey, M.R. and Power, J.B. 1994. Isolation, culture and regeneration of protoplasts. In: Dixon RA and Gonzales RA (eds), Plant Cell Culture - A Practical Approach, pp.27-38, Oxford University Press.
6. Davey, M.R. 1983. Recent developments in the culture and regeneration of plant protoplasts. Experiment. Suppl. 46: 19-20.
7. Gürel, E. 1997. Callus and root development from leaf explants of sugar beet (Beta vulgaris L.): Variability between cultivars, plants and organs. Turkish J Bot. 21: 131-136.
8. Gürel, S., Gürel, E. And Kaya, Z. 2001a. Establishment of cell suspension cultures and plant regeneration in sugar beet (Beta vulgaris L.) Turkish J Bot. (In Press)
9. Gürel, S., Gürel, E. And Kaya, Z. 2001b. Callus development and indirect shoot regeneration from seedling explants of sugar beet (Beta vulgaris L.) cultured in vitro. Turkish J Bot, 25: 25-33.
10. Hall, R.D., Pedersen, C. and Krens, F.A. 1993a. Improvement of protoplast culture protocols for Beta vulgaris L. (sugar beet). Plant Cell Rep. 12: 339-342.
11. Hall, R.D., Pedersen, C. and Krens, F.A. 1993b. Progress towards the development of a general somatic hybridization protocol for Beta. J. Sugar Beet Res. 30(4): 275-290.
12. Krens, F.A., Jamar, D., Rouwendal, G.J.A. and Hall, R.D. 1990. Transfer of cytoplasm from new Beta CMS sources to sugar beet by asymmetric fusion. I. Shoot regeneration from mesophyll protoplasts and characterization of regenerated plants. Theor. Appl. Genet. 79: 390-396.
13. Lenzler, S., Zoglauer, K. and Schieder, O. 1995. Plant regeneration from protoplasts of sugar beet (Beta vulgaris). Physiol. Planta. 94: 342-350.
14. Majewska-Sawka, A., Nakashima, H. and Mori, K. 1990. Protoplast isolation from mesophyll of Beta species. Proc. Jap. Soc. Beet Technol. 32: 129-133.
15. Majewska-Sawka, A., Nakashima, H. and Mori, K. 1994. Isolation and culture of suspension-derived protoplasts of Beta vulgaris L. Biologia Planta. 36(1): 9-13.
16. Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Planta. 15: 473-497.
17. Pedersen, C., Hall, R.D. and Krens, F.A. 1993. Petioles as the tissue source for isolation and culture of Beta vulgaris and Beta maritima protoplasts. Plant Sci. 95: 89-97.
18. Sairam, R.V., Seetharama, N., Devi, P.S., Verma, A., Murthy, U.R. and Potrykus, I. 1999. Culture and regeneration of mesophyll-derived protoplasts of sorghum (Sorghum bicolor L.) Moench. Plant Cell Rep. 18: 972-977.
19. Schlangstedt, M., Zoglauer, K., Lenzler, S., Hermans, B. and Jacobs, M. 1994. Improvement of sugar beet (Beta vulgaris L.) protoplast culture: Leaf petioles as a protoplast source. J. Plant Physiol. 143: 227-233.
20. Vasil, I.K. and Vasil, V. 1980. Isolation and culture of protoplasts. In: IK Vasil (ed), Perspectives in Plant Cell and Tissue Culture. 11B, pp. 1-9, Int. Rev. Cytol. Suppl.
21. Warren, G. 1996. Protoplast isolation and fusion. In: Stafford A and Warren G (eds), Plant Cell and Tissue Culture, pp. 48-81, John Wiley and Sons, Chiecster, UK