Identification of the EcoKI and EcoR124I type I restriction-modification enzyme subunits by non-equilibrium pH gradient two-dimensional gel electrophoresis

Folia Microbiologica

Volumn 47, Issue 6, 2002, Pages 641-648

  Nguyen, L D ^a   Cajthamlova K ^a   Nguyen, H T ^a   Weiser, J ^a   Holubova I ^a   Weiserova M ^a  

^a INSTITUTE OF MICROBIOLOGY   (Czech Republic)

Author keywords

[No Author keywords available]

Indexed keywords

DNA MODIFICATION METHYLASE ECOKI; ECOR124 MODIFICATION METHYLASE TYPE I; ENDODEOXYRIBONUCLEASE ECOKI; ENDODEOXYRIBONUCLEASE ECOR124I; RESTRICTION ENDONUCLEASE; SITE SPECIFIC DNA METHYLTRANSFERASE (ADENINE SPECIFIC); TYPE I SITE SPECIFIC DEOXYRIBONUCLEASE;

ARTICLE; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; ISOELECTRIC POINT; MOLECULAR WEIGHT; TWO DIMENSIONAL GEL ELECTROPHORESIS; WESTERN BLOTTING;

BLOTTING, WESTERN; DEOXYRIBONUCLEASES, TYPE I SITE-SPECIFIC; DNA RESTRICTION ENZYMES; DNA RESTRICTION-MODIFICATION ENZYMES; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ESCHERICHIA COLI; ISOELECTRIC POINT; MOLECULAR WEIGHT; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC);

EID: 0041369966     PISSN: 00155632     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02818664     Document Type: Article

References (28)

1. APPLEYARD R.K.: Segregation of new lysogenic types during growth of doubly lysogenic strains derived from Escherichia coli K-12. Genetics 39, 440-452 (1954).
2. BARCUS V.A., TITHERADGE A.J., MURRAY N.E.: The diversity of alleles at the hsd locus in natural populations of Escherichia coli. Genetics 140, 1187-1197 (1995).
3. BRADFORD M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal.Biochem. 72, 248-254 (1976).
4. DYBVIG K., HUILAN Y.: Regulation of a restriction and modification system via DNA inversion in Mycoplasma pulmonis. Mol.Microbiol. 12, 547-560 (1994).
5. FIRMAN K., PRICE C., GLOVER S.W.: The EcoR124 and EcoR124/3 restriction and modification systems: cloning the genes. Plasmid 14, 224-234 (1985).
6. FIRMAN K.D., DUTTA C., WEISEROVá M., JANščáK P.: The role of subunit assembly in the functional control of type I restriction -modification enzymes. Mol.Biol.Today 1, 34-41 (2000).
7. HUBáčEK J., GLOVER S.W.: Complementation analysis of temperature-sensitive host specificity mutations in Escherichia coli. J.Mol. Biol. 50, 111-127 (1970).
8. JANščáK P., DRYDEN D.T., FIRMAN K.: Analysis of the subunit assembly of the type IC restriction-modification enzyme EcoR124I. Nucl.Acids Res. 26, 4439-4445 (1998).
9. KOBAYASHI I., NOBUSATO A., KOBAYASHI-TAKAHASHI N., UCHIYAMA I.: Shaping the genome-restriction-modification systems as mobile genetic elements. Curr.Opin.Genet.Dev. 9, 649-656 (1999).
10. LAEMMLI U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685 (1970).
11. LI X.M., VOHRADSKÝ J., WEISER J.: The use of protein synthesis elongation factor EF-Tu as internal calibration standard in two-dimensional electrophoretic studies of differentiation in Streptomyces. Electrophoresis 15, 1198-1204 (1994).
12. LOPEZ M.F.: Nonequilibrium pH gel electrophoresis (NEPHGE). Meth.Mol.Biol. 112, 129-131 (1999).
13. MURRAY N.E.: Type I restriction systems: sophisticated molecular machines (a legacy of Bertani and Weigle). Microbiol.Mol.Biol.Rev. 64, 412-434 (2000).
14. O'FARRELL P.H.: High resolution two-dimensional electrophoresis of proteins. J.Biol.Chem. 250, 4007-4021 (1975).
15. O'FARRELL P.Z., GOODMAN H.M., O'FARRELL P.H.: High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell 12, 1133-1141 (1977).
16. PATEL J., TAYLOR I., DUTTA C.F., KNEALE G., FIRMAN K.: High-level expression of the cloned genes encoding the subunits of and intact DNA methyltransferase, M.EcoR124. Gene 112, 21-27 (1992).
17. PRAKASH-CHENG A., CHUNG S.S., RYU J.: The expression and regulation of hsdK genes after conjugative transfer. Mol.Gen.Genet. 241, 491-196 (1993).
18. RABILLOUD T.: A comparison between low background silver diammine and silver nitrate protein stains. Electrophoresis 13, 429-439 (1992).
19. REDASCHI N., BICKLE T.A.: Posttranscriptional regulation of EcoP11 and EcoP15I restriction activity. J.Mol.Biol. 257, 790-803 (1996).
20. SAMBROOK J.C., FRJTSCH E.F., MANIATIS T.: Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor (USA) 1989.
21. SAUNDERS N.J., PEDEN J.F., HOOD D.W., MOXON E.R.: Simple sequence repeats in the Helicobacter pylori genome. Mol.Microbiol. 27, 1091-1098 (1998).
22. TAYLOR I., PATEL J., FIRMAN K., KNEALE G.: Purification and biochemical characterization of the EcoR124 type I modification methylase. Nucl.Acids Res. 20, 179-186 (1992).
23. TITHERADGE A.J., TERNENT D., MURRAY N.B.: A third family of allelic hsd genes in Salmonella enterica: sequence comparisons with related proteins identify conserved regions implicated in restriction of DNA. Mol.Microbiol. 22, 437-447 (1996).
24. TOMB J.F., WHITE O., KERLAVAGE A.R., CLAYTON R.A., SUTTON G.G., FLEISCHMANN R.D., KETCHUM K.A., KLENK H.P., GILL S., DOUGHERTY B.A., NELSON K., QUACKENBUSH J., ZHOU L., KIRKNESS E.F., PETERSON S., LOFTUS B., RICHARDSON D., DODSON R., KHALAK H.G., GLODEK A., MCKENNEY K., FITZEGERALD L.M., LEE N., ADAMS M.D., HICKEY E.K., BERG D.E., GOCAYNE J.D., UTTERBACK T.R., PETERSON J.D., KELLEY J.M., COTTON M.D., WEIDMAN J.M., FUJI C., BOWMAN C., WATTHEY L., WALLIN E., HAYES W.S., BORODOVSKY M., KARP P.D., SMITH H.O., FRASER C.M., VENTER J.C.: The complete genome sequence of the gastric pathogen Helicobacter pylori. Nature 388, 539-547 (1997).
25. WEISER J., MIKULÍK K., žIžKA Z., šŤASTNá J., JANDA I., JIRáŇOVá A.: Isolation and characterization of Streptomyces aureofaciens protein-synthesis elongation factor Tu in an aggregated state. Eur.J.Biochem. 129, 127-132 (1982).
26. WEISEROVá M., JANščáK P., BENADA O., HUBáčEK J., ZINKEVICH V.E., GLOVER S.W., FIRMAN K.: Cloning, production and charactenzation of wild-type and mutant forms of the R.EcoK endonucleases. Nucl.Acids Res. 21, 373-379 (1993); published erratum: Nucl.Acids Res. 21, 1686 (1993).
27. YANISCH-PERRON C., VIEIRA J., MESSING J.: Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103-119 (1985).
28. ZEINDL-EBERHART E., JUNGBLUT P.R., RABES H.M.: A new method to assign immunodetected spots in the complex two-dimensional electrophoresis pattern. Electrophoresis 18, 799-801 (1997).