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Stable Bond C18 was donated by Agilent Technologies.
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The gradient acetonitrile/water/TFA treatment used was 7.5/87.5/5 for 2 min over 16 min and 47.5/7.5/5 for 7 min at 150 °C and 2 mL/min. This treatment also removes residual aluminum (<40 ppb Al(III) remains) from the FC alkylations.
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