Flow cytometry characterization of white cell-reduced blood: Apoptosis markers and morphology of postfiltration elements

Vox Sanguinis

Volumn 85, Issue 2, 2003, Pages 109-113

  Tazzari, P L ^a   Bontadini, A ^a   Fruet, F ^a   Tassi, C ^a   Ricci, F ^a   Manfroi, S ^a   Conte, Roberto ^a,b  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b Serv Immunoematol e Med Trasfus   (Italy)

Author keywords

Apoptosis; Filtered packed red blood cells; Flow cytometry

Indexed keywords

BENZYLOXYCARBONYLVALYLALANYLASPARTYL FLUOROMETHYL KETONE; BIOCHEMICAL MARKER; FLUORESCEIN ISOTHIOCYANATE; HLA ANTIGEN CLASS 1; LEUKOCYTE ANTIGEN; MONOCLONAL ANTIBODY; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE INHIBITOR;

APOPTOSIS; ARTICLE; BLOOD TRANSFUSION; CELL DEATH; CELL POPULATION; CELL SELECTION; CYTOTOXIC T LYMPHOCYTE; ERYTHROCYTE CONCENTRATE; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNE RESPONSE; IMMUNOFLUORESCENCE; LEUKOCYTE COUNT; PHAGOCYTOSIS; PRIORITY JOURNAL;

APOPTOSIS; BIOLOGICAL MARKERS; CELL SEPARATION; CELL SIZE; ERYTHROCYTE TRANSFUSION; FILTRATION; FLOW CYTOMETRY; HUMANS; IMMUNOPHENOTYPING; LEUKOCYTES; TIME FACTORS;

EID: 0041336827     PISSN: 00429007     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1423-0410.2003.00326.x     Document Type: Article

References (15)

1. Kerr JF, Wyllie AH, Currie AR: Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 1972; 26:239-257
2. Cohen JJ: Apoptosis. Immunol Today 1993; 14:126-130
3. Allen RT, Hunter WJ III, Agrawal DK: Morphological and biochemical characterization and analysis of apoptosis. J Pharmacol Toxicol Methods 1997; 37:215-228
4. Martelli AM, Zweyer M, Ochs RL, Tazzari PL, Narducci P, Bortul R: Nuclear apoptotic changes. An overview. J Cell Biochem 2001; 82:634-646
5. Snyder EL, Kuter DJ: Apoptosis in transfusion medicine: of death and dying - is that all there is? Transfusion 2000; 40:135-138
6. Frabetti F, Musiani D, Marini M, Fanelli C, Coppola S, Ghibelli L, Tazzari PL, Bontadini A, Tassi C, Conte R: White cell apoptosis in packed red cells. Transfusion 1998; 38:1082-1089
7. Bontadini A, Tazzari PL, Manfroi S, Tassi C, Conte R: Apoptosis in leukodepleted packed red blood cells. Vox Sang 2002; 86:704-715
8. Martelli AM, Tazzari PL, Bortul R, Riccio M, Tabellini G, Santi S, Frabetti F, Musiani D, Bareggi R, Conte R: Nuclear matrix protein is released from apoptotic white cells during cold (1-6 °C storage of concentrated red cell units and might induce antibody response in multiply transfused patients. Transfusion 2000; 40:169-177
9. Pistillo MP, Tazzari PL, Gaudiano C, Cilla V, Kato T, Matsui T, Nishioka K, Capanni P, Conte R, Ferrara GB: Detection of anti-CTLA-4 antibodies in sera from multiply transfused patients with hematological diseases. Transfusion 2001; 41:462-469
10. Nicoletti I, Migliorati G, Pagliacci MC, Grignani F, Riccardi C: A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods 1991; 139:271-279
11. Koester SK, Roth P, Mikulka WR, Schlossman SF, Zhnag C, Bolton B: Monitoring early cellular responses in apoptosis is aided by the mitochondrial membrane protein-specific monoclonal antibody AP02.7. Cytometry 1997; 29:1086-1092
12. Duriez PJ, Shah GM: Cleavage of poly (ADP-ribose) polymerase: a sensitive parameter to study cell death. Biochem Cell Biol 1997; 75:337-349
13. Sowemimo-Coker SO, Kim A, Tribble E, Brandwein HJ, Wenz B: White cell subsets in apheresis and filtered platelet concentrates. Transfusion 1998; 38:650-657
14. Vamvakas E, Blaichman M: Universal WBC reduction: the case for and against. Transfusion 2001; 41:691-712
15. Sweeney J: Universal leukoreduction of cellular blood components in 2001? Yes. Am J Clin Pathol 2001; 115:666-673