Copper/zinc-superoxide dismutase from Epinephelus malabaricus cDNA and enzyme property

Journal of Agricultural and Food Chemistry

Volumn 51, Issue 19, 2003, Pages 5688-5694

  Ken, Chuian Fu ^a   Cheng, Yu Feng ^a   Chang, Ching Fong ^a   Lin, Chi Tsai ^a  

^a NATIONAL TAIWAN OCEAN UNIVERSITY   (Taiwan)

Author keywords

Copper zinc superoxide dismutase (Cu Zn SOD); Echerichia coli; Epinephelus malabaricus; Expression; PCR; PET 20b(+)

Indexed keywords

COMPLEMENTARY DNA; COPPER ZINC SUPEROXIDE DISMUTASE;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ASSAY; ENZYME INDUCTION; ENZYME PURIFICATION; EPINEPHELUS MALABARICUS; FISH; MARINE ENVIRONMENT; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; TAIWAN; WATER POLLUTANT; WATER POLLUTION;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ENZYME ACTIVATION; ENZYME STABILITY; GENE EXPRESSION; HYDROGEN-ION CONCENTRATION; KINETICS; MOLECULAR SEQUENCE DATA; PERCIFORMES; SUPEROXIDE DISMUTASE;

EPINEPHELUS; EPINEPHELUS MALABARICUS; ESCHERICHIA COLI; VECTORS;

EID: 0041333146     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf030162p     Document Type: Article

References (18)

1. Brock, C. J.; Walker, J. E. Superoxide dismutase from Bacillus stearothermophilus. Complete amino acid sequence of a manganese enzyme. Biochemistry 1980, 19, 2873-2882.
2. Fridovich, I. Superoxide dismutases. Adv. Enzymol. 1986, 58, 61-97.
3. Harris, J. I.; Auffret, A. D.; Northrop, F. D.; Walker, J. E. Structural comparisons of superoxide dismutases. Eur. J. Biochem. 1980, 106, 297-303.
4. Okamoto, O. K.; Robertson, D. L.; Fagan, T. F.; Hastings, J. W.; Colepicolo, P. Different regulatory mechanisms modulate the expression of a dinoflagellate iron-superoxide dismutase. J. Biol. Chem. 2001, 276, 19989-19993.
5. Buhler, D. R.; Williams, D. E. The role of biotransformation in the toxicity of chemicals. Aquat. Toxicol. 1988, 11, 19-28.
6. Livingstone, D. R. Towards a specific index of impact by organic pollution for marine invertbrates. Comp. Biochem. Physiol. 1991, 100C, 151-155.
7. Winston, G. W.; Di Giulio, R. T. Prooxidant and antioxidant mechanisms in aquatic organisms. Aquat. Toxicol. 1991, 19, 137-161.
8. Sies, H. Biochemistry of oxidative stress. Angew. Chem. Int. Ed. Engl. 1986, 25, 1058-1071.
9. Rodriguez-Ariza, A.; Abril, N.; Navas, J. I.; Dorado, G.; Lopez-Barea, J.; Pueyo, C. Metal, mutagenicity, and biochemical studies on bivalve molluscs from Spanish coasts. Environ. Mol. Mutagen. 1992, 19, 112-124.
10. Rodriguez-Ariza, A.; Dorado, G.; Peinado, J.; Pueyo, C.; Lopez-Barea, J. Biochemical effects of environmental pollution in fishes from Spanish South-Atlantic littoral. Biochem. Soc. Trans. 1991, 19, 301S.
11. Pedrajas, J. R.; Peinado, J.; Lopez-Barea, J. Purification of Cu, Zn-superoxide dismutase isoenzymes from fish liver: appearance of new isoforms as a consequence of pollution. Free Radical Commun. 1993, 19, 29-41.
12. Dimitrova, M. St.; Tishinova, V.; Velcheva, V. Combined effect of zinc and lead on the hepatic superoxide dismutase-catalase system in carp (Cyprinus carpio). Comp. Biochem. Physiol. 1994, 108C, 43-46.
13. Calabrese, L.; Polticelli, F.; O'Neill, P.; Galtieri, A.; Barra, D.; Schinina, E.; Bosssa, F. Substitution of arginine for lysine 134 alters electrostatic parameters of the active site in shark Cu/Zn superoxide dismutase. FEBS Lett. 1989, 250, 49-52.
14. Ken, C. F.; Shaw, J. F.; Wu, J. L.; Lin, C. T. Molecular cloning of a cDNA coding for a Cu/Zn-superoxide dismutase from zebrafish and overexpression in Escherichia coli. J. Agric. Food Chem. 1998, 46, 2863-2867.
15. Lin, C. T.; Lee, T. L.; Duan, K. J.; Ken, C. F. Molecular cloning, characterization and expression of a cDNA coding copper/zinc-superoxide dismutase from black porgy. J. Agric. Food Chem. 2000, 48, 4444-4447.
16. Strauss, W. M. Hybridization with radioactive probes. Current Protocols in Molecular Biology; Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., Struhl, K., Eds.; Current Protocols: U.S.A., 1993; pp 6.3.1-6.3.6.
17. Beauchamp, C.; Fridovich, I. Improved assays and an assay applicable to acrylamide gel. Anal. Biochem. 1971, 44, 276-287.
18. Lütcke, H. A.; Chow, K. C.; Mickel, F. S.; Moss, K. A.; Kern, H. F. ; Scheele, G. A. Selection of AUG initiation codons differs in plants and animals. EMBO J. 1987, 6, 43-48.