Potent suppression of HIV type 1 infection by a short hairpin anti-CXCR4 siRNA

AIDS Research and Human Retroviruses

Volumn 19, Issue 8, 2003, Pages 699-706

  Anderson, Joseph ^a   Banerjea, Akhil ^a   Planelles, Vicente ^b   Akkina, Ramesh ^a  

^a Veterinary Dentistry and Oral Surgery   (United States)

^b UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMOKINE RECEPTOR CXCR4; DNA DIRECTED DNA POLYMERASE ALPHA; LENTIVIRUS VECTOR; RNA; SMALL INTERFERING RNA; UNCLASSIFIED DRUG;

ANTIVIRAL ACTIVITY; ARTICLE; CELL SURFACE; CONTROLLED STUDY; DOWN REGULATION; DRUG DESIGN; DRUG SYNTHESIS; GENE ACTIVITY; GENE DISRUPTION; GENE SILENCING; GENE STRUCTURE; GENE THERAPY; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; IN VITRO STUDY; PRIORITY JOURNAL; PROTEIN TARGETING; VIRAL GENE DELIVERY SYSTEM; VIRUS ADSORPTION; VIRUS RESISTANCE; WESTERN BLOTTING;

BLOTTING, WESTERN; CELLS, CULTURED; FLOW CYTOMETRY; GENE SILENCING; GENE THERAPY; HIV INFECTIONS; HIV-1; HUMANS; RECEPTORS, CXCR4; RNA, SMALL INTERFERING;

EID: 0041322735     PISSN: 08892229     EISSN: None     Source Type: Journal    
DOI: 10.1089/088922203322280928     Document Type: Article

References (30)

1. Cagnon L and Rossi J: Down regulation of the CCR5 beta-chemokine receptor and inhibition of HIV-1 infection by stable VA1-ribozyme chimerical transcripts. Antisense Nucleic Acid Drug Dev 2001;10:251-261.
2. Lui D, Donesgan J, Nuovo G, Mitra D, and Laurence J: Stable human immunodeficiency virus type 1 (HIV-1) resistance in transformed CD4+ monocytic cells treated with multitargeting HIV-1 antisense sequences incorporated into U1 snRNA. J Virol 1997;71:4079-4085.
3. Bahner I, Kearns K, Hao Q-L, Smogorzewska EM, and Kohn DB: Transduction of human CD34+ hematopoietic progenitor cells by a retroviral vector expressing an RRE decoy inhibits human immunodeficiency virus type 1 replication in myelomonocytic cells produced in long term culture. J Virol 1996;70:4352-4360.
4. Lisziewicz J, Sun D, Smythe J, Lusso P, Lori F, Louie A, Markham P, Rossi J, Reitz M, and Gallo RC: Inhibition of human immunodeficiency Virus type 1 replication by regulated expression of a polymeric Tat activation response RNA decoy as a strategy for gene therapy in AIDS. Proc Natl Acad Sci USA 1993;90: 8000-8004.
5. Bai J, Gorantla S, Banda N, Cagnon L, Rossi J, and Akkina R: Characterization of anti-CCR5 ribozyme-transduced CD34+ hematopoietic progenitor cells in vitro and in a SCID-hu mouse model in vivo. Mol Ther. 2002;1:244-254.
6. Bai J, Rossi J, and Akkina R: Multivalent anti-CCR5 ribozymes for stem cell-based HIV Type 1 gene therapy. AIDS Res Hum Retroviruses 2001;17:385-399.
7. Feng Y, Leavitt M, Tritz R, Duarte E, Kang D, Mamounas M, Gilles P, Wong-Staal F, Kennedy S, Merson J, Yu M, and Barber JR: Inhibition of CCR5-dependent HIV-1 infection by hairpin ribozyme gene therapy against CC-chemokine receptor 5. Virology 2000;276:271-278.
8. Rossi JJ: The application of ribozymes to HIV infection. Curr Opin Mol Ther 1999;1:316-322.
9. Bonyhadi M, Moss K, Voytovich A, Auten J, Kalfoglou C, Plavec I, Forestell S, Su L, Bohnlein E, and Kaneshima H: RevM10-expressing T cells derived in vivo from transduced human hematopoietic stem-progenitor cells inhibit human immunodeficiency virus replication. J Virol 1997;71:47074-716.
10. Ding SF, Lombardi R, Nazari R, and Joshi S: A combination anti-HIV-1 gene therapy approach using a single transcription unit that expresses antisense, decoy, and sense RNAs, and transdominant negative mutant Gag and Env proteins. Front Biosci 2002;7: a15-28.
11. Ulich C, Harrich D, Estes P, and Gaynor RB: Inhibition of human immunodeficiency virus type 1 replication is enhanced by a combination of transdominant Tat and Rev proteins. J Virol 1996;70: 4871-4876.
12. Banda N, Akkina RK, Terrell K, Shpall EJ, Tomczak J, Campain J, Clamam H, Cagle L, and Harrison GS: Diphtheria toxin A gene-mediated HIV-1 protection of cord blood-derived T cells in the SCID-hu mouse model. J Hematother 1998;7:319-331.
13. Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, and Tuschl T: Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 2001;411:494-498.
14. Hannon GJ: RNA interference. Nature 2002;418:244-251.
15. Sharp PA: RNA interference-2001. Genes Dev 2001;15:485-490.
16. Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, and Mello CC: Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 1998;391:806-811.
17. Coburn GA and Cullen BR: Potent and specific inhibition of human immunodeficiency virus type 1 replication by RNA interference. J Virol 2002;76:9225-9231.
18. Jacque J, Triques K, and Stevenson M: Modulation of HIV-1 replication by RNA interference. Nature 2002;418:379-380.
19. Lee NS, Dohjima T, Bauer G, Li H, Li M-J, Ehsani A, Salvaterra P, and Rossi J: Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells. Nat Biotechnol 2002; 19:500-505.
20. Novina CD, Murray MF, Dykxhoom DM, Beresford PJ, Reiss J, Lee S, Collman RG, Lieberman J, Shankar P, and Sharp PA: siRNA-directed inhibition of HIV-1 infection. Nat Med 2002;8: 681-686.
21. Qin XF, An DS, Chen IS, and Baltimore D: Inhibiting HIV-1 infection in human T cells by lentiviral-mediated delivery of small interfering RNA against CCR5. Proc Natl Acad Sci USA 2003;100: 183-188.
22. Berger EA, Murphy PM, and Farber JM: Chemokine receptors as HIV-1 Coreceptors: Roles in viral entry, tropism, and disease. Annu Rev Immunol 1999;17:657-700.
23. Endres MJ, Clapham PR, Marsh M, Ahuja M, Turner JD, McKnight A, Thomas JF, Stoebenau-Haggarty B, Choe S, Vance PJ, Wells TN, Power CA, Sutterwala SS, Doms RW, Landau NR, and Hoxie JA: CD4-independent infection by HIV-2 is mediated by fusin/CXCR4. Cell 1996;87:745-756.
24. Brummelkamp TR, Bernards R, and Agami R: A system for stable expression of short interfering RNAs in mammalian cells. Science 2002;296:550-553.
25. Paddison PJ, Caudy AA, Bernstein E, Harmon GJ, and Conklin DS: Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes Dev 2002;16:948-958.
26. Donze O and Picard D: RNA interference in mammalian cells using siRNAs synthesized with T7 RNA polymerase. Nucleic Acids Res 2002;30:e46.
27. Vodicka MA, Goh WC, Wu LI, Rogel ME, Bartz SR, Schweickart VL, Raport CJ, and Emerman M: Indicator cell lines for detection of primary strains of human and simian immunodeficiency viruses. Virology 1997;233:193-198.
28. Kimpton J and Emerman M: Detection of replication-competent and pseudotyped human immunodeficiency virus with a sensitive cell line on the basis of activation of an integrated β-galactosidase gene. J Virol 1992;66:2232-2239.
29. Martinez MA, Gutierrez A, Armand-Ugon M, Blanco J, Parera M, Gomez J, Clotet B, and Este JA: Suppression of chemokine receptor expression by RNA interference allows for inhibition of HIV-1 replication. AIDS 2002;16:2385-2390.
30. Sioud M and Leirdal M: Gene silencing in mammalian cells by preformed RNA duplexes. Biochem Biopbys Res Commun 2002; 295:744-748.