Sequential fluorescent staining with CMA and DAPI for somatic chromosome identification in cucumber (Cucumis sativus L.)

Journal of Applied Genetics

Volumn 39, Issue 3, 1998, Pages 249-258

  Plader, Wojciech ^a   Hoshi, Yoshikazu ^a   Malepszy, Stefan ^a  

^a WARSAW UNIVERSITY OF LIFE SCIENCES   (Poland)

Author keywords

Chromosome identification; CMA; Cucumis sativus; DAPI

Indexed keywords

EID: 0041133196     PISSN: 12341983     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (19)

1. ARUMUGANATHAN K., EARLE E.D. (1991). Estimation of nuclear DNA content of plants by flow cytometry. Plant Mol. Biol. Report 9: 229-241.
2. AYYANGAR K.R. (1967). Taxonomy of the Cucurbitaceae. Bull. Nat. Inst. Sci. India 34: 380-396.
3. BHADURI P.N., BOSE P.C. (1947). Cytogenetical investigation in some cucurbits with special reference to fragmentation of chromosomes as a physical basis of speciation. J. Genet. 48: 237-256.
4. DAGA R.R., THODE G., AMORES A. (1996). Chromosome complement, C-banding, Ag-NOR and replication banding in the zebrafish Danio rerio. Chromosome Res. 4: 29-32.
5. DEUMLING B. (1981). Sequence arrangement of a highly methylated satellite DNA of a plant, Scilla: a tandemly repeated inverted inverted repeat. Proc. Nat. Acad. Sci. USA 78: 338-442.
6. DEUMLING B., GREILHUBER J. (1982). Characterization of heterochromatin in different species of the Scilla siberica group (Liliaceae) by in situ hybridization of the satellite DNAs and fluorochrome banding. Chromosoma 84: 535-555.
7. GUERRA M.S. (1988). Mitotic and meiotic analysis of a pericentric inversion associated with a tandem duplication in Eleutherine bulbosa. Chromosoma 97: 80-87.
8. HOSHI Y., PLADER W., MALEPSZY S. (1998). New C-banding pattern for chromosome identification in cucumber (Cucumis sativus L.). Plant Breeding 117: 77-82.
9. KENTON A. (1991). Heterochromatin accumulation, disposition and diversity in Gibasis krwinskayana (Commelinaceae). Chromosoma 100: 467-478.
10. MARIE D., BROWN S. (1993). A cytometric exercise in plant DNA histograms, with 2C values for 70 species. Biol. Cell 78: 41-51.
11. PORTUGAL J., WARING M.J. (1988). Assignment of DNA binding sites for 4′,6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative study. Biochim. Biophys. Acta 949: 158-168.
12. RAMACHANDRAN C., SESHADRI V.S. (1986). Cytological analysis of the genome of cucumber (Cucumis sativus L.) and muskmelon (Cucumis melo L.). Z. Pflanzenzucht. 96: 25-38.
13. SCHWEIZER D. (1976). Reverse fluorescent chromosome banding with chromomycin and DAPI. Chromosoma 58: 307-324.
14. SCHWEIZER D. (1981). Counterstain-enhanced chromosome banding. Hum. Genet. 57: 1-14.
15. SINGH A.K. (1990). Cytogenetics and evolution in the Cucurbitaceae. In: Biology and Utilization of the Cucurbitaceae (Baates D.M., Robinson R., Jeffry C., eds.) pp. 10-28. Cornell University Press, Itahaca and London.
16. SINGH A.K., ROY R.P. (1974). Karyological studies in Cucumis L. Caryologia 27: 153-160.
17. TRIVEDI R.N., ROY R.P. (1970). Cytological studies in Cucumis and Citrus. Cytologia 35: 561-569.
18. WARD D.C., REICH E., GOLDBERG I.H. (1965). Base specificity in the interaction of polynucleotides with antibiotic drugs. Science 149: 1259-1263.
19. XU J., EARLE E.D. (1996). High resolution physical mapping of 45S (5.8S, 18S and 25S) DNA gene loci in the tomato genome using a combination of karyotyping and FISH of pachytene chromosome. Chromosoma 104: 545-550.