Development of a chromosome-plasmid balanced lethal gene expression system of Vibrio cholerae based on thyA locus

Chinese Journal of Microbiology and Immunology

Volumn 20, Issue 3, 2000, Pages 223-227

  Xia, Xiaobin ^a   Qi, Guoming ^a   Liu, Yanqing ^a  

^a CHINESE CENTER FOR DISEASE CONTROL AND PREVENTION   (China)

Author keywords

Chromosome plasmid balanced lethal system; Gene expression; Thymidylate synthase gene; Vibrio cholerae

Indexed keywords

EID: 0041110539     PISSN: 02545101     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. Makalanos JJ, Sadoff JC. Cholera Vaccines: fighting an ancient scourge. Science, 1994, 265:1387-1389.
2. + expression-cloning vector: stable maintenance and high level expression of cloned genes in a Salmonella vaccine strain. Biotechnology, 1988, 6:693-697.
3. Okada T, Yanagisawa K, Ryan FJ. Elective production of thymine-less mutants. Nature (London), 1960, 188:340-341.
4. Belfort M, Maley G, Pedersen-Lane J, et al. Primary structure of the E. coli thyA gene and its thymidylate synthase product. Proc Natl Acad Sci USA, 1983, 80:4914-4918.
5. Marcus H, Ketley JM, Kaper JB, et al. Effects of DNase production, plasmid size, and restriction barriers on transformation of Vibrio cholerae by electroporation and osmotic shock. FEMS Microbiol Lett, 1990, 68: 149-154.
6. Okada T, Homma J, Sonohara H. Improved method for obtaining thymineless mutants of E. coli and Salmonella typhimurium. J Bacteriol, 1962, 84:602-603.
7. Ross P, O' Gara F and Condon S, Thymidylate synthase gene from Lactococcus Lactis as a genetic marker: an alternative to antibiotic resistance genes. Appl Environ Microbiol, 1990, 56(7):2164-2169.
8. Andrew MHE. Use of trimethoprim to obtain thymine-requiring mutants of Streptococcus faecalis. J Gen Microbiol, 1973, 74:195-199.
9. Kelln RA, Warren RAJ. Obligate thymicine auxotrophs of Pseudomonas acidovorans. J Bacteriol, 1973, 113(1):510-511.
10. Blomberg L, Henriksson A, Conway PL. Inhibition of adhension of E. coli K88 to piglet ileal mucus by Lactobacillus spp. Appl Environ Microbiol, 1993, 59(1):34-39.
11. Farmer JL, Rothman F. Transformable thymine-requiring mutant of Bacillus subtilis. J Bacteriol, 1965, 89(1):262-263.