Introducing stuI sites improves vectors for the expression of fusion proteins with factor Xa cleavage sites

Gene

Volumn 170, Issue 1, 1996, Pages 145-146

  Görlach, Jörn ^a,b   Schmid, Jürg ^a  

^a ETH ZURICH   (Switzerland)

^b Ciba Geigy Corporation   (United States)

Author keywords

Escherichia coli; Expression vector; Factor Xa; Fusion protein; Recombinant DNA

Indexed keywords

BLOOD CLOTTING FACTOR 10A; HYBRID PROTEIN; RECOMBINANT DNA;

ARTICLE; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION; NONHUMAN; PRIORITY JOURNAL; RESTRICTION SITE;

EID: 0041094210     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(95)00825-X     Document Type: Article

References (4)

1. Appert, C., Logemann, E., Hahlbrock, K., Schmid, J. and Amrhein, N.: Structural and catalytic properties of the four phenylalanine ammonia-lyase isoenzymes from parsley (Petroselinum crispum Nym.). Eur. J. Biochem. 225 (1994) 491-499.
2. Hochuli, E., Bannwarth, W., Döbeli, H., Gentz, R. and Stüber, D.: Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent. Bio/Technology 6 (1988) 1321-1325.
3. Schmid, J., Schaller, A., Leibinger, U., Boll, W. and Amrhein, N.: The in-vitro synthesized tomato shikimate kinase precursor is enzymatically active and is imported and processed to the mature enzyme by chloroplasts. Plant J. 2 (1992) 375-383.
4. Smith, D.B. and Johnson, K.S.: Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67 (1988) 31-40.