Identification of the photobionts in Trapeliopsis and Pertusaria using SSU ribosomal DNA sequences obtained from PCR amplification with a non-green-algal primer

Mycotaxon

Volumn 78, Issue , 2001, Pages 407-411

  Schmitt, Imke ^a   Lumbsch, H Thorsten ^a  

^a UNIVERSITY OF DUISBURG ESSEN   (Germany)

Author keywords

18S rDNA; Lichens; Molecular phytogeny; Primer

Indexed keywords

EID: 0041079294     PISSN: 00934666     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Castresana, J. (2000) Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol. Biol. Evol. 17: 540-552.
2. Crespo, A., Bridge, P. D. & Hawksworth, D. L. (1997) Amplification of fungal rDNA-ITS regions from non-fertile specimens of the lichen-forming genus Parmelia. Lichenologist 29: 275-282.
3. Coring, H., Clerc, P., Grube, M. & Wedin, M. (2000) Mycobiont specific PCR primers for the amplification of nuclear ITS and LSU rDNA from lichenised ascomycetes. Lichenologist 32: 200-204.
4. Felsenstein, J. (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783-791.
5. Gardes, M. & Bruns, T.D. (1993) ITS primers with enhanced specificity for basidiomycetes - application to the identification of mycorrhizae and rusts. Mol. Ecol. 2: 113-118.
6. Gargas, A. & DePriest, P. (1996) A nomenclature for fungal PCR primers with examples from intron-containing SSU rDNA. Mycologia 88: 745-748.
7. Gargas, A. & Taylor, J. W. (1992) Polymerase chain reaction (PCR) primers for amplifying and sequencing nuclear 18S rDNA from lichenized fungi. Mycologia 84: 589-592.
8. Lumbsch, H.T. & Schmitt, I. (2000) Molecular data suggest that the lichen genus Pertusaria is not monophyletic. Lichenologist, submitted.
9. Mankin, A. S., Skryabin, K. G. & Rubtsov, P. M. (1986) Identification often additional nucleotides in the primary structure of yeast 18S rRNA. Gene 44: 143.
10. Page, R. D. M. (1996) Treeview: an application to display phylogenetic trees on personal computers. Comp. Appl. Biosci. 12: 357-358.
11. Rubtsov, P. M., Musakhanov, M. M., Zakharyev, V. M., Krayev, A. S., Skryabin, K. G. & Bayev, A. A. (1980) The structure of the yeast ribosomal RNA genes. I. The complete nucleotide sequence of 18S ribosomal RNA gene from Saccharomyces cerevisiae. Nucl. Acids Res. 8: 5779-5794.
12. Swofford, D. L. (1998) PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version 4. Sinauer Associates, Sunderland, Mass.
13. Takeshita, S., Nakano, T. & Iwatsuki, Z. (1989) Phycobionts of some Japanese species of Pertusaria (Pertusariaceae). Pl. Syst. Evol. 165: 49-54.
14. Thompson, J. D., Higgins, D. G. & Gibson, T. J. (1994) Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl. Acids Res. 22: 4673-4680.
15. Tschermak-Woess, E. (1978) Über den Chlorella-Phycobionten von Trapelia coarctata. Pl. Syst. Evol. 130: 253-263.
16. White, T. J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: a guide to methods and applications. (M. A. Innis, D. H. Gelfand, J. J. Sninsky & T. J. White, eds): 315-322. Academic Press, San Diego.