Quantification of low levels of human immunodeficiency virus (HIV) type 1 RNA in P24 antigen-negative, asymptomatic, HIV-positive patients by PCR

Journal of Clinical Microbiology

Volumn 34, Issue 2, 1996, Pages 404-408

  Angeles Munoz Fernandez M ^a   Navarro, Joaquín ^a   Montes, Miguel García ^a   Cosín, Jaime ^a   Zabay, Jose María ^a   Fernández Cruz, Eduardo ^a  

^a HOSPITAL GENERAL UNIVERSITARIO GREGORIO MARAÑÓN   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA INTERFERON; ANTIGEN P24; HUMAN IMMUNODEFICIENCY VIRUS ANTIGEN; VIRUS RNA; ZIDOVUDINE;

ANTIVIRAL ACTIVITY; ARTICLE; CLINICAL ARTICLE; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ASSAY; TREATMENT OUTCOME; VIRUS PARTICLE;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 0040864424     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.34.2.404-408.1996     Document Type: Article

References (16)

1. Albert, J., and E. M. Fenyo. 1990. Simple, sensitive, and specific detection of human immunodeficiency virus type 1 in clinical specimens by polymerase chain reaction with nested primers J. Clin Microbiol. 28:1560-1564
2. Becker-Andre, M., and K. Hahlbrock. 1989 Absolute mRNA quantification using the polymerase chain reaction (PCR). A novel approach by a PCR aided transcript titration assay (PATTY) Nucleic Acids Res 17:9437-9446.
3. Chomczynski, P., and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanatc-phenol-chloroform extraction. Anal. Bio-chem 162:156-159.
4. Coombs, R. W., A. C. Collier, J. P. Allain, B. Kikora, M. Leuther, G. F. Gjerset, and L. Corey. 1989. Plasma viremia in human immunodeficiency virus infection N Engl. J. Med 321:1626-1631.
5. Fernández-Cruz, E., M. Desco, M. García Montes, N. Longo, B. González, and J. M. Zabay. 1990. Immunological and serotogical markets predictive of progression to AIDS in a cohort of HIV-infected drug users. AIDS 4: 987-994.
6. Gonzalez, B., M. García Montes, J. M. Zabay, N. Longo, J. M. Sempere, and E. Fernández-Cruz. 1991. Circulating immune complexes (CIC) in HIV-infected drug users (IVDU). Demonstration of p24 antigen as a component of CIC in patients with and without free-antigen throughout the course of HIV infection, abstr. W A. 1143, p. 127. In Abstracts of the VIIth International Conference on AIDS. Florence. Italy. 1991.
7. Holodniy, M., D. A. Katzenstein, D. M. Israelski, and T. C. Merigan. 1991. Reduction an plasma human immunodeficiency virus ribonucleic acid after dicleoxynucleoside therapy as determined by the polymerase chain reaction. J. Clin. Invest 88:1755-1759
8. Ilolodniy, M., D. A. Katzenstein, S. Sengupta, et al. 1991. Detection and quantification of human immunodeficiency virus RNA in patient's serum by use of the polymerase chain reaction J Infect Dis 163:862-866
9. Holodniy, M., M. A. Winters, and T. C. Merigan. 1992. Detection and quantification of gene amplification products by a nonisotopic automated system BioTechniques 12:38-39.
10. Jurriaans, S., J. T. Dekker, and A. de Ronde. 1992 HIV-1 viral DNA load in peripheral blood mononuclear cells from seroconverteis and long-term infected individuals. AIDS 6:635-641.
11. Kwok, S., and R. Higuchi. 1989. Avoiding false positives, with PCR. Nature (London) 339:237-238
12. Munoz, A., M. C. Wang, S. Bass, J. M. Taylor, L. A. Kingsley, J. S. Chmiel, and B. F. Polk. 1989. Aequired immunodeficiency syndrome (AIDS)-free time after human immunodeficiency virus type 1 (HIV-1) seroconversion in homosexual men. Multicenter AIDS Cohort Study Group. Am. J. Epidemiol. 130:530-539.
13. Pantaleo, G., C. Graziosi, and A. S. Fauci. 1993 New concepts in the immunopathogenesis of human immunodeficiency virus infection. N. Engl. J. Med. 328:327-335.
14. Piatak, M., M. S. Saag, L. C. Yang, et al. 1993. High levels of HIV-1 in plasma during all stages of infection determined by competitive PCR. Science 259:1749-1754.
15. + lymphocyte levels, J. Infect. Dis. 164:72-80.
16. Scadden, D. T., Z. Wang, and J. E. Groopman. 1992. Quantitation of plasma human immunodeficiency virus type 1 RNA by competitive polymerase chain reaction. J. Infect. Dis. 165:1119-1123.