Functional and genetic characterization of mcpC, which encodes a third methyl-accepting chemotaxis protein in Bacillus subtilis

Microbiology

Volumn 143, Issue 10, 1997, Pages 3231-3240

  Müller, Jakob ^a   Schiel, Stacey ^b   Ordal, George W ^b   Saxild, Hans H ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

Chemotaxis receptor protein; mcpC expression; mcpC gene sequence; Methylation; (D) dependent expression

Indexed keywords

ARGININE; BACTERIAL PROTEIN; BETA GALACTOSIDASE; CHEMOTACTIC PEPTIDE; CYSTEINE; GLYCINE; LYSINE; PROLINE; RECEPTOR PROTEIN; SERINE; THREONINE; VALINE;

ARTICLE; BACILLUS SUBTILIS; CHEMOTAXIS; GENE CASSETTE; GENETIC TRANSCRIPTION; MOLECULAR WEIGHT; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN METHYLATION; SEQUENCE HOMOLOGY;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); TRIXIS;

EID: 0040822641     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-143-10-3231     Document Type: Article

References (37)

1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. (1990). Basic local alignment search tool. J Mol Biol 215, 403-410.
2. Birnboim, H. C. & Doly, J. (1979). A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7, 1513-1523.
3. Bischoff, D. S. & Ordal, G. W. (1991). Sequence and characterization of Bacillus subtilis CheB, a homolog of Escherichia coli CheY, and its role in different mechanism of chemotaxis. J Biol Chem 266, 12301-12305.
4. Boylan, R. J., Mendelson, N. H., Brooks, D. & Young, F. E. (1972). Regulation of the bacterial cell wall: analysis of a mutant of Bacillus subtilis defective in biosynthesis of teichoic acid. J Bacteriol 110, 281-290.
5. Errington, J. (1986). A general method for fusion of the Escherichia coli lacZ gene to chromosomal genes in Bacillus subtilis. J Gen Microbiol 132, 2953-2966.
6. Fajardo-Cavazos, P., Salazar, C. & Nicholson, W. L. (1993). Molecular cloning and characterization of the Bacillus subtilis spore photoproduct lyase (spl) gene, which is involved in repair of UV radiation-induced DNA damage during spore germination. J Bacteriol 175, 1735-1744.
7. D-dependent gene encodes a novel protein with both CheW and CheY homologous domains. J Bacteriol 176, 2727-2735.
8. Fuhrer, D. K. & Ordal, G. W. (1991). Bacillus subtilis CheN, a homolog of CheA, the central regulator of chemotaxis in Escherichia coli. J Bacteriol 173, 7443-7448.
9. Gilman, M. Z., Wings, J. L. & Chamberlin, M. J. (1981). Nucleotide sequence of two Bacillus subtilis promoters used by Bacillus subtilis sigma-28 RNA polymerase. Nucleic Acids Res 9, 5991-6000.
10. Hanahan, D. (1983). Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166, 557-580.
11. Hanlon, D. W. & Ordal, G. W. (1994). Cloning and characterization of genes encoding methyl-accepting chemotaxis proteins in Bacillus subtilis. J Biol Chem 269, 14038-14046.
12. Hanlon, D. W., Márquez-Magana, L. M., Carpenter, P. B., Chamberlin, M. J. & Ordal, G. W. (1992). Sequence and characterization of Bacillus subtilis CheW. J Biol Chem 267, 12055-12060.
13. Hanlon, D. W., Rosario, M. M. L., Ordal, G. W., Venema, G. & van Sinderen, D. (1994). Identification of TlpC, a novel 62 kDa MCP-like protein from Bacillus subtilis. Microbiology 140, 1847-1854.
14. Hedblom, M. L. & Adler, J. (1980). Genetic and biochemical properties of Escherichia coli mutants with defects in serine chemotaxis. J Bacteriol 144, 1048-1060.
15. Itaya, M. (1993). Physical map of the Bacillus subtilis 168 chromosome. In Bacillus subtilis and Other Gram-Positive Bacteria: Biochemistry, Physiology, and Molecular Genetics, pp. 463-471. Edited by A. L. Sonenshein, J. A. Hoch & R. Losick. Washington, DC: American Society for Microbiology.
16. Kirsch, M. L., Peters, P. D., Hanlon, D. W., Kirby, J. R. & Ordal, G. W. (1993a). Chemotactic methylesterase promotes adaptation to high concentrations of attractant in Bacillus subtilis. J Biol Chem 268, 18610-18616.
17. Kirsch, M. L., Zuberi, A. R., Henner, D., Peters, P. D., Yazdi, M. A. & Ordal, G. W. (1993b). Chemotactic methyltransferase promotes adaptation to repellents in Bacillus subtilis. J Biol Chem 268, 25350-25356.
18. Kossmann, M., Wolff, C. & Manson, M. D. (1988). Maltose chemoreceptor of Escherichia coli : interaction of maltose-binding protein and the Tar signal transducer. J Bacteriol 170, 4516-4521.
19. Kyte, J. & Doolittle, R. F. (1982). A simple method for displaying the hydropathic character of a protein. J Mol Biol 157, 105-132.
20. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 211, 680-685.
21. 14C in polyacrylamide gels by fluorography. Eur J Biochem 56, 335-341.
22. Le Moual, H. & Koshland, D. E., Jr (1996). Molecular evolution of the C-terminal cytoplasmic domain of a superfamily of bacterial receptors involved in taxis. J Mol Biol 261, 568-585.
23. D expression and activity by spo0, abrB, and sin gene products in Bacillus subtilis. J Bacteriol 176, 2435-2438.
24. Miller, J. H. (1972). Assay of β-galactosidase. In Experiments in Molecular Genetics, pp. 352-355. Cold Spring Harbor, NY : Cold Spring Harbor Laboratory.
25. Ordal, G. W. & Goldman, D. J. (1975). Chemotaxis away from uncouplers of oxidative phosphorylation in Bacillus subtilis. Science 189, 802-804.
26. Ordal, G. W., Villani, D. P. & Gibson, K. J. (1977). Amino acid chemoreceptors of Bacillus subtilis. J Bacteriol 129, 156-165.
27. Ordal, G. W., Márquez-Magana, L. & Chamberlin, M. J. (1993). Motility and chemotaxis. In Bacillus subtilis and Other Gram-Positive Bacteria, pp. 765-784. Edited by A. L. Sonenshein, J. A. Hoch & R. Losick. Washington, DC: American Society for Microbiology.
28. Rosario, M. M., Fredrick, K. L., Ordal, G. W. & Helmann, J. D. (1994). Chemotaxis in Bacillus subtilis requires either of two functionally redundant CheW homologs. J Bacteriol 176, 2736-2739.
29. Rosario, M. M. L. & Ordal, G. W. (1996). CheC and CheD interact to regulate methylation of Bacillus subtilis methyl-accepting chemotaxis proteins. Mol Microbiol 21, 511-518.
30. Sanger, F., Nicklen, S. & Coulson, A. R. (1977). DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74, 5463-5467.
31. Saxild, H. H. & Nygaard, P. (1987). Genetic and physiological characterization of Bacillus subtilis mutants resistant to purine analogs. J Bacteriol 169, 2977-2983.
32. Saxild, H. H., Jensen, C. L., Hubrechts, P. & Hammer, K. (1994). Isolation and characterization of Bacillus subtilis genomic lacZ fusions induced during partial purine starvation. J Bacteriol 176, 276-283.
33. Saxild, H. H., Jacobsen, J. H. & Nygaard, P. (1995). Functional analysis of the Bacillus subtilis purT gene encoding formate-dependent glycinamide ribonucleotide transformylase. Microbiology 141, 2211-2218.
34. Saxild, H. H., Andersen, L. N. & Hammer, K. (1996). dra-nupC-pdp operon of Bacillus subtilis: nucleotide sequence, induction by deoxyribonucleosides, and transcriptional regulation by the deoR-encoded DeoR repressor protein. J Bacteriol 178, 424-434.
35. Ullah, A. H. & Ordal, G. W. (1981). In vivo and in vitro chemotactic methylation in Bacillus subtilis. J Bacteriol 145, 958-965.
36. Youngman, P., Perkins, J. B. & Losick, R. (1984). A novel method for the rapid cloning in Escherichia coli of Bacillus subtilis chromosomal DNA adjacent to Tn917 insertions. Mol Gen Genet 195, 424-433.
37. Zuberi, A. R., Ying, C., Parker, H. M. & Ordal, G. W. (1990). Transposon Tn917lacZ mutagenesis of Bacillus subtilis: identification of two new loci required for motility and chemotaxis. J Bacteriol 172, 6841-6848.