Functional analysis of genes responsible for the synthesis of the B-band O antigen of pseudomonas aeruginosa serotype O6 lipopolysaccharide

Microbiology

Volumn 145, Issue 12, 1999, Pages 3505-3521

  Bélanger, Myriam ^a   Burrows, Lori L ^a,b   Lam, Joseph S ^a  

^a UNIVERSITY OF GUELPH   (Canada)

^b TORONTO GENERAL HOSPITAL   (Canada)

Author keywords

Lipopolysaccharide; O antigen; Pseudomonas aeruginosa; rfb; wbp

Indexed keywords

BACTERIAL ANTIGEN; BACTERIUM LIPOPOLYSACCHARIDE; GLYCOSYLTRANSFERASE; N ACETYLGALACTOSAMINE; N ACETYLGLUCOSAMINE; URIDINE DIPHOSPHATE N ACETYLGALACTOSAMINE;

ARTICLE; BACTERIAL GENE; BACTERIUM MUTANT; GENE CLUSTER; GENE MUTATION; GENETIC ANALYSIS; GENETIC COMPLEMENTATION; KNOCKOUT GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; SALMONELLA TYPHI; SEQUENCE HOMOLOGY; SEROTYPE;

ANIMALIA; BACTERIA (MICROORGANISMS); NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA; SALMONELLA TYPHI;

EID: 0040496902     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-145-12-3505     Document Type: Article

References (61)

1. Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25, 3389-3402.
2. Arsenault, T. L., Hughes, D. W., MacLean, D. B., Szarek, W. A., Kropinski, A. M. B. & Lam, J. S. (1991). Structural studies on the polysaccharide portion of 'A-band' lipopolysaccharide from a mutant (AK 1401) of Pseudomonas aeruginosa strain PAO1. Can J Chem 69, 1273-1280.
3. Bélanger, M. & Lam, J. S. (1998). Molecular organization and characterization of genes required for the synthesis of Pseudomonas aeruginosa O6 B-band lipopolysaccharide. In 12th Annual North American Cystic Fibrosis Conference, Oct. 15-18, Montreal, QC, abstract no. 384.
4. Berry, D. & Kropinski, A. M. (1986). Effect of lipopolysaccharide mutations and temperature on plasmid transformation efficiency in Pseudomonas aeruginosa. Can J Microbiol 32, 436-438.
5. Bert, F. & Lambert-Zechovsky, N. (1996). Comparative distribution of resistance patterns and serotypes in Pseudomonas aeruginosa isolates from intensive care units and other wards. J Antimicrob Chemother 37, 809-813.
6. Birnboim, H. C. & Doly, J. (1979). A rapid alkaline extraction procedure for screening recombinant plasmids. Nucleic Acids Res 7, 1513-1523.
7. Burrows, L. L. & Lam, J. S. (1999). Effect of wzx (rfbX) mutations on A-band and B-hand lipopolysaccharide biosynthesis in Pseudomonas aeruginosa O5. J Bacteriol 181, 973-980.
8. Burrows, L. L., Charter, D. F. & Lam, J. S. (1996). Molecular characterization of the Pseudomonas aeruginosa serotype O5 (PAO1) B-band lipopolysaccharide gene cluster. Mol Microbiol 22, 481-495.
9. Burrows, L. L., Chow, D. & Lam, J. S. (1997). Pseudomonas aeruginosa B-band O antigen chain length is modulated by Wzz (Rol). J Bacteriol 179, 1482-1489.
10. Clarke, B. R., Bronner, D., Keenleyside, W. J., Severn, W. B., Richards, J. C. & Whitfield, C. (1995). Role of Rfe and RfbF in the initiation of biosynthesis of D-galactan I, the lipopolysaccharide O antigen from Klebsiella pneumoniae serotype O1. J Bacteriol 177, 5411-5418.
11. Comstock, L. E., Johnson, J. A., Michalski, J. M., Morris, J. G., Jr & Kaper, J. P. (1996). Cloning and sequence of a region encoding a surface polysaccharide of Vibrio cholerae O139 and characterization of the insertion site in the chromosome of Vibrio cholerae O1. Mol Microbiol 19, 815-826.
12. Cryz, S., Jr, Pitt, T. L., Furer, E. & Gremanier, R. (1984). Role of lipopolysaccharide in virulence of Pseudomonas aeruginosa. Infect Immun 44, 508-513.
13. Curd, H., Liu, D. & Reeves, P. R. (1998). Relationships among the O antigen gene clusters of Salmonella enterica groups B, D1, D2, and D3. J Bacteriol 180, 1002-1007.
14. Dean, C. R., Franklund, C. V., Retief, J. D., Coyne, M. J., Jr, Hatano, K., Evans, D. J., Pier, G. B. & Goldberg, J. B. (1999). Characterization of the serogroup O11 O antigen locus of Pseudomonas aeruginosa PA103. J Bacteriol 181, 4275-4284.
15. Dubray, G. & Bezard, G. (1982). A highly sensitive periodic acid-silver stain for 1,2-diol groups of glycoproteins and polysaccharides in polyacrylamide gels. Anal Biochem 119 , 325-329.
16. K genes encode antibodies to Pseudomonas aeruginosa LPS. J Immunol 155, 3912-3921.
17. Ford, N., Nolan, C. & Ferguson, M. (1989). Transfer of DNA to nitrocellulose filters. In Molecular Cloning: a Laboratory Manual, 2nd edn, pp. 9.38-9.40. Edited by J. Sambrook, E. F. Fritsch & T. Maniatis. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
18. Fotheringham, I. G., Dacey, S. A., Taylor, P. P., Smith,T. J., Hunter, M. G., Finlay, M. E., Primrose, S. B., Parker, D. M. & Edwards, R. M. (1986). The cloning and sequence analysis of the aspC and tyrB genes from Escherichia coli K12. Comparison of the primary structures of the aspartate aminotransferase and aromatic aminotransferase of Escherichia coli with those of the pig aspartate aminotransferase isoenzymes. Biochem J 234, 593-604.
19. Geremia, R. A., Petroni, E. A., Ielpi, L. & Henrissat, B. (1996). Towards a classification of glycosyltransferases based on amino acid sequence similarities: prokaryotic α-mannosyltransferascs. Biochem J 318, 133-138.
20. Goldberg, J. B. & Ohman, D. E. (1984). Cloning and expression in Pseudomonas aeruginosa of a gene involved with the production of alginate. J Bacteriol 158, 1115-1121.
21. Goldberg, J. B. & Pier, G. B. (1996). Pseudomonas aeruginosa lipopolysaccharides and pathogenesis. Trends Microbiol 4, 490-494.
22. Goldberg, J. B., Hatano, K., Meluleni, G. S. & Pier, G. B. (1992). Cloning and surface expression of P. aeruginosa O antigen in E. coli. Proc Natl Acad Sci USA 89, 10716-10720.
23. Hancock, R. E. W. & Carey, A. M. (1979). Outer membrane of Pseudomonas aeruginosa: heat- and 2-mercaptoethanol-modifiable proteins. J Bacteriol 158, 115-1121.
24. Hashimoto, Y., Li, N., Yokoyama, H. & Ezaki, T. (1993). Complete nucleotide sequence and molecular characterization of viaB region encoding Vi antigen in Salmonella typhi. J Bacteriol 175, 4456-4465.
25. Hitchcock, P. J. & Brown, T. M. (1983). Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol 154, 269-277.
26. Hoang, T. T., Karkhoff-Schweizer, R. R., Kutchma, A. J. & Schweizer, H. P. (1998). A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants. Gene 212, 77-86.
27. Houng, H.-S. H. & Venkatesan, M. M. (1998). Genetic analysis of Shigella sonnei form I antigen: identification of a novel IS630 as an essential element for the form I antigen expression. Microb Pathog 25, 165-173.
28. Huff, J. P., Grant, B. J., Penning, C. A. & Sullivan, K. F. (1990). Optimization of routine transformation of Escherichia coli with plasmid DNA. BioTechniques 9, 570-577.
29. de Kievit, T. R., Dasgupta, T., Schweizer, H. & Lam, J. S. (1995). Molecular cloning and characterization of the rfc gene of Pseudomonas aeruginosa (serotype O5). Mol Microbiol 16, 565-574.
30. Knirel, Y. A. (1990). Polysaccharide antigens of Pseudomonas aeruginosa. Crit Rev Microbiol 17, 273-304.
31. Knirel, Y. A. & Kochetkov, N. K. (1994). The structure of lipopolysaccharides of gram-negative bacteria. III. The structure of O antigens: a review. Biochemistry 59, 1325-1383.
32. Lightfoot, J. L. & Lam, J. S. (1993). Chromosomal mapping, expression and synthesis of lipopolysaccharide in Pseudomonas aeruginosa: a role for guanosine diphospho (GDP)-D-mannose. Mol Microbiol 8, 771-782.
33. Liu, P. V. & Wang, S. P. (1990). Three new major somatic antigens of Pseudomonas aeruginosa. J Clin Microbiol 28, 922-925.
34. Liu, D., Cole, R. A. & Reeves, P. R. (1996). An O antigen processing function for Wzx (RfbX): a promising candidate for O-unit flippase. J Bacteriol 178, 2102-2107.
35. Liu, P. V., Matsumoto, H., Kusama, H. & Bergan, T. (1983). Survey of heat-stable major somatic antigens of Pseudomonas aeruginosa. Int J Syst Bacteriol 33, 256-264.
36. Ma, S., Selvaraj, U., Ohman, D. E., Quarless, R., Hassett, D. J. & Wozniak, D. J. (1998). Phosphorylation-independent activity of the response regulators AlgB and AlgR in promoting alginate biosynthesis in mucoid Pseudomonas aeruginosa, J Bacteriol 180, 956-968.
37. MacIntyre, S., Lucken, R. & Owen, P. (1986). Smooth lipopolysaccharide is the major protective antigen for mice in the surface extract from IATS serotype 6 contributing to the polyvalent Pseudomonas aeruginosa vaccine PEV. Infect Immun 52, 76-84.
38. Macpherson, D. F., Manning, P. A. & Morona, R. (1994). Characterization of the dTDP rhamnose bioynthetic genes encoded in the rfb locus of Shigella flexneri. Mol Microbiol 11, 281-292.
39. Masoud, H., Sadovskaya, I., De Kievit, T., Altman, E., Richards, J. C. & Lam, J. S. (1995). Structural elucidation of the lipopolysaccharide core region of the O-chain-defident mutant strain A28 from Pseudomonas aeruginosa serotype O6 (International Antigenic Typing Scheme). J Bacteriol 177 , 6718-6726.
40. May, T. B., Shinabarger, D., Maharaj, R. & 9 other authors (1991). Alginare synthesis by Pseudomonas aeruginosa: a key pathogenic factor in chronic pulmonary infections of cystic fibrosis patients. Clin Microbiol Rev 4, 191-206.
41. Naide, Y., Nikaido, H., Mäkelä, P. H., Wilkinson, R. G. & Stocker, B. A. D. (1965). Semirough strains of Salmonella. Proc Natl Acad Sci USA 53, 147-153.
42. Nakai, Y., Hayashi, H. & Kagamiyama, H. (1996). Cloning and characterization of the tyrB gene from Salmonella typhimurium. Biochim Biophys Acta 1308, 189-192.
43. Pitt, T. L. (1988). Epidemiological typing of Pseudomonas aeruginosa. Eur J Clin Microbiol Infect Dis 7, 238-247.
44. Reeves, P. (1993). Evolution of Salmonella O antigen variation by interspecific gene transfer on a large scale. Trends Genet 9, 17-22.
45. Reeves, P. R., Hobbs, M., Valvano, M. A. & 8 other authors (1996). Bacterial polysaccharide synthesis and gene nomenclature. Trends Microbiol 4, 495-503.
46. Rocchetta, H. L., Burrows, L. L., Pacan, J. C. & Lam, J. S. (1998). Three rhamnosyltransferases responsible for assembly of the A-band D-rhamnan polysaccharide in Pseudomonas aeruginosa: a fourth transferase, WbpL, is required for initiation of both A-band and B-band LPS synthesis. Mol Microbiol 28, 1103-1119.
47. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
48. Schnaitman, C. A. & Klena, J. D. (1993). Genetics of lipopolysaccharide biosynthesis in enteric bacteria. Microbiol Rev 57, 655-682.
49. Schweizer, H. P. (1993). Small broad-host-range gentamicin resistance gene cassettes for site-specific insertion and deletion mutagenesis. BioTechniques 15, 831-833.
50. Schweizer, H. P. (1994). A method for construction of bacterial hosts for Lac-based cloning and expression vectors: α-complementation and regulated expression. BioTechniques 17, 452-456.
51. Schweizer, H. P. & Hoang, T. T. (1995). An improved system for gene replacement and xyIE fusion analysis in Pseudomonas aeruginosa. Gene 158, 15-22.
52. Simon, R., Priefer, U. & Pühler, A. (1983). A broad-host-range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram-negative bacteria. Bio/Technology 1, 784-791.
53. Skurnik, M., Venho, R., Toivanen, P. & Al-Hendy, A. (1995). A novel locus of Yersinia enterocolitica serotype O:3 involved in lipopolysaccharide outer core biosynthesis. Mol Microbiol 17, 575-594.
54. Stroeher, U. H., Parasivam, G., Dredge, B. K. & Manning, P. A. (1997). Novel Vibrio cholerae O139 genes involved in lipopolysaccharide biosynthesis. J Bacteriol 179, 2740-2747.
55. Vachee, A., Scheftel, J. M., Husson, M. O., Izard, D., Ross, P. & Monteil, H. (1997). Tricentric study of the sensitivity of Pseudomonas aeruginosa serotyping to beta-lactams and aminoglycosides. Pathol Biol 45, 357-362.
56. Virlogeux, I., Waxin, H., Ecobichon, C. & Popoff, M. Y. (1995). Role of the viaB locus in synthesis, transport and expression of Salmonella typhi Vi antigen. Microbiology 141, 3039-3047.
57. Waxin, H., Virlogeux, I., Kolyva, S. & Popoff, M. Y. (1993). Identification of six open reading frames in the Salmonella enterica subsp. enterica ser. Typhi viaB locus involved in Vi antigen production. Res Microbiol 144, 363-371.
58. West, S. E. H., Schweizer, H. P., Dall, C., Sample, A. K. & Runyen-Janecky, L. J. (1994). Construction of improved EscherichiaPseudomonas shuttle vectors derived from pUC18/19 and the sequence of the region required for their replication in Pseudomonas aeruginosa. Gene 128, 81-86.
59. Woods, D. E., Lam, J. S., Paranchych, W., Speert, D. P., Campbell, M. & Godfrey, A. J. (1997). Correlation of Pseudomonas aeruginosa virulence factors from clinical and environmental isolates with pathogenicity in the neutropenic mouse. Can J Microbiol 43, 541-551.
60. Wyk, P. & Reeves, P. R. (1989). Identification and sequence of the gene for ahequose synthetase which confers antigenic specificity on group B Salmonellae: homology with galactose epimerase. J Bacteriaol 171, 5687-5693.
61. Zhang, L., Radziejewska-Lebrecht, J., Krajewska-Pietrasik, D., Toivanen, P. & Skurnik, M. (1997). Molecular and chemical characterization of the lipopolysaccharide O antigen and its role in the virulence of Yersinia enterocolitica serotype O:8. Mol Microbiol 23, 63-76.