Fluorescence in situ hybridization (FISH) screening of frozen cell lines in large numbers

BioTechniques

Volumn 26, Issue 3, 1999, Pages 408-412

  Lo, Anthony W I ^a,b   Choo, K H Andy ^a  

^a MURDOCH CHILDREN'S RESEARCH INSTITUTE   (Australia)

^b ROYAL CHILDREN'S HOSPITAL   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT;

ANALYTIC METHOD; ARTICLE; CELL CULTURE; CELL LINE; CHROMOSOME ANALYSIS; CHROMOSOME MAP; CONTROLLED STUDY; DNA DETERMINATION; DNA HYBRIDIZATION; FLUORESCENCE IN SITU HYBRIDIZATION; FREEZING; HUMAN; HUMAN CELL;

EID: 0040314988     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99263bm08     Document Type: Article

References (6)

1. Barry, A.E., E.V. Howman, M.R. Cancilla, R. Saffery and K.H.A. Choo. Sequence analysis of an 80-kb human neocentromere. Hum. Mol. Genet. (In press).
2. Choo, K.H.A. 1994. In Situ Hybridization Protocols. Humana Press, Totowa.
3. Gosden, J. and G. Scopes. 1996. Uncultured blood samples can be labeled by PRINS and ready for chromosome enumeration analysis 1 h after collection. BioTechniques 21:88-91.
4. Kuo, W.-L., H. Tenjin, R. Segraves, D. Pinkel, M.S. Golbus and J. Gray. 1991. Detection of aneuploidy involving chromosomes 13, 18, and 21, by fluorescence in situ hybridization (FISH) to interphase and metaphase amniocytes. Am. J. Hum. Genet. 49:112-119.
5. Phelan, M.C. 1997. Techniques for mammalian cell culture, p. A.3F.1-A.3F.11. In F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman and K. Struhl (Eds.), Current Protocols in Molecular Biology. John Wiley & Sons, New York.
6. Pinkel, D., T. Sraume and J.W. Gray. 1986. Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc. Natl. Acad. Sci. USA 85:2934-2938.