The redox centers in the molybdo iron-sulfur flavoprotein CO dehydrogenase from the thermophilic carboxidotrophic bacterium Pseudomonas thermocarboxydovorans

FEMS Microbiology Letters

Volumn 176, Issue 1, 1999, Pages 139-145

  Hänzelmann, Petra ^a   Hofmann, Bettina ^a   Meisen, Sabine ^a   Meyer, Ortwin ^a  

^a UNIVERSITY OF BAYREUTH   (Germany)

Author keywords

Carbon monoxide; Carboxidotrophic bacterium; CO dehydrogenase; Molybdenum cofactor; Molybdopterin cytosine dinucleotide

Indexed keywords

CARBON MONOXIDE DEHYDROGENASE; CYTOSINE; DIMER; DINUCLEOTIDE; FLAVINE ADENINE NUCLEOTIDE; IRON SULFUR PROTEIN; MOLYBDENUM;

ARTICLE; COMPLEX FORMATION; ELECTRON SPIN RESONANCE; NONHUMAN; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL; PSEUDOMONAS; THERMOPHILIC BACTERIUM;

EID: 0040009307     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(99)00230-X     Document Type: Article

References (25)

1. Meyer, O. (1982) Chemical and spectral properties of carbon monoxide:methylene blue oxidoreductase. J. Biol. Chem. 257, 1333-1341.
2. Meyer, O., Frunzke, K. and Mörsdorf, G. (1993) Biochemistry of the aerobic utilization of carbon monoxide. In: Microbial Growth on Ci Compounds (Murreil, J.C. and Kelly, D.P., Eds.), pp. 433-459. Intercept Scientific, Andover.
3. Meyer, O., Frunzke, K., Tqchil, J. and Volk, M. (1993) The bacterial molybdenum cofactor. In: Molybdenum Enzymes, Cofactors, and Model Systems (Stiefel, E.I., Coucouvanis, D. and Newton, W.E., Eds.), pp. 50-68. ACS Symposium Series 535, American Chemical Society, Washington, DC.
4. Hänzelmann, P. and Meyer, O. (1998) Effect of molybdate and tungstate on the biosynthesis of CO dehydrogenase and the molybdopterin cytosine dinucleotide-type of molybdenum cofactor in Hyilrogenopliaga pscudoflava. Eur. J. Biochcm 255, 755-765.
5. Schübel, U., Kraut, M., Mörsdorf, G. and Meyer, O. Molecular characterization or the gene cluster coxMSL encod. ing the molybdenum-containing carbon monoxide dehydrogenäse 'of Oligotropha carboxidowrcms. J. Bacteriol. 177 2197-2203.
6. Dobbek, H., Gremer, L., Meyer, O. and Huber, R. (1991) Crystal structure of CO dehydrogenase, a molybdo iron-sulfut flavoprotein containing S-selanylcysteine. Nature, submitted.
7. Bell, J.M., Williams, E. and Colby, J. (1985) Carbon monox ide oxidoreductase from thermophilic carboxydobacteria. In: Microbial Gas Metabolism (Poole, R.K. and Dow, C.S., Eds.), pp. 153-160. Academic Press, London.
8. Pearson, D.M., O'Reilly, C, Colby, J. and Black, G.W. (1994) DNA sequence of the au A, B and C genes, encoding the molybdenum containing hydroxylase carbon monoxide dehydrogenase, from Pseudomonas tlicrnwcarboxydomrans strain C2. Biochim. Biophys. Acta 1188, 432-438.
9. Hille, R. (1996) Structure and function of mononuclear molybdenum enzymes. J. Biol. Inorg. Chem. 1, 397-404.
10. Wierenga, R.K., De Maeyer, M.C.H. and Hol, W.G.J. (1985) Interaction of pyrophosphate moieties with a-helices in dinucleotide binding proteins. Biochemistry 24, 1346-1357.
11. Romào, M.J., Archer, M., Moura, L, Moura, J.J.G., LeGal], J., Engh, R., Schneider, M., Hof, P. and Huber, R. (1995) Crystal structure of the xanthine oxidase-related aldehyde oxido-reductase from D. gigas. Science 270, 1170-1176.
12. Meyer, O. and Schlegel, H.G. (1983) Biology of aerobic carbon monoxide-oxidizing bacteria. Annu. Rev. Microbiol. 37, 277-310.
13. Lyons, C.M., Justin, P., Colby, J. and Williams, E. (1984) Isolation, characterization and autotrophic metabolism of a moderately thermophilic carboxydobacterium, Pseudomonas thermocarboxydovorans sp. nov. J. Gen. Microbiol. 130, 1097-1105.
14. Kraut, M., Hugendieck, I., Herwig, S. and Meyer, O. (1989) Homology and distribution of CO dehydrogenase structural genes in carboxydotrophic bacteria. Arch. Microbiol. 152, 335-341.
15. Johnson, J.L. and Rajagopalan, K.V. (1982) Structural and metabolic relationship between the molybdenum cofactor and urothione. Proc. Natl. Acad. Sci. USA 79, 6856-6860.
16. Johnson, J.L., Bastian, N.R. and Rajagopalan, K.V. (1990) Molybdopterin guanine dinucleotide: A modified form of molybdopterin identified in the molybdenum cofactor of dimethl sulfoxide reductase from Rhodobacter sphaeroides forma specialis denitrificans. Proc. Natl. Acad. Sci. USA 87, 3190-3194.
17. Laemmli, U.K. (1970) Cleavage of structural proteins durinc the assembly of the head of bacteriophage T4. Nature 227, 680-685.
18. Gremer, L. and Meyer, O. (1996) Characterization of xanthine dehydrogenase from the anaerobic bacterium Veillonella aiypica and identification of a molybdopterin-cytosine-dinucleotide-containing molybdenum cofactor. Eur. J. Biochem. 238, 862-866.
19. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72, 248-254.
20. Fish, W.W. (1988) Rapid colorimetric micromethod for the quantitation of complexed iron in biological samples. Methods Enzymol. 158, 357-364.
21. Cardenas, J. and Mortenson, L.E. (1974) Determination of molybdenum and tungsten in biological materials. Anal. Biochem. 60, 372-381.
22. Foeo, J.K. and Popowsky, M. (I949) Spectrophotometric determination of hydrogen sulfide. Anal. Chcm. 21, 732-734.
23. Bray, R.C., George, G.N., Lange, R. and Meyer, O. (1983) Studies by e.p.r. spectroscopy of carbon monoxide oxidases from Pseudomonas carboxydororans and Psetidomonas carboxydohydrogcna. Biochem. J. 211, 687-694.
24. Meyer, O. and Rajagopalan, K.V. (1984) Molybdopterin in carbon monoxide oxidase from carboxydotrophic bacteria. J. Bacteriol. 157, 643-648.
25. Bray, R.C. (1975) Molybdenum iron-sulfur flavin hydroxylases and related enzymes. In: The Enzymes (Boyer, P.D., Ed.), pp. 300-419. Academic Press, New York.