Negative regulation of the SHATTERPROOF genes by FRUITFULL during Arabidopsis fruit development

Science

Volumn 289, Issue 5478, 2000, Pages 436-438

  Ferrándiz, Cristina ^a   Liljegren, Sarah J ^a   Yanofsky, Martin F ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARABIDOPSIS; ARTICLE; GENE CONTROL; GENE EXPRESSION REGULATION; NONHUMAN; PLANT GROWTH; PLANT SEED; PRIORITY JOURNAL;

ARABIDOPSIS; DNA-BINDING PROTEINS; GENE EXPRESSION REGULATION, PLANT; GENES, PLANT; MADS DOMAIN PROTEINS; MUTATION; PHENOTYPE; PLANT COMPONENTS; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; RNA, PLANT; SEEDS; TRANSCRIPTION FACTORS;

ARABIDOPSIS; BRASSICA NAPUS VAR. NAPUS; SPERMATOPHYTA;

EID: 0039842580     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.289.5478.436     Document Type: Article

References (16)

1. J. Spence, Y. Vercher, P. Gates, N. Harris, J. Microsc. 181, 195 (1996).
2. Q. Gu, C. Ferrándiz, M. Yanofsky, R. Martienssen, Development 125, 1509 (1998).
3. A. M. Mandel and M. F. Yanofsky, Plant Cell 7, 1763 (1995).
4. P. N. Benfey and N.-H. Chua, Science 250, 959 (1990).
5. The FUL cDNA was cloned into the pBIN-JIT vector, a pBIN19 (Clontech) derivative, which placed FUL transcription under the control of a tandem repeat of the 355 promoter. Eighteen of 28 independent transformants in the Columbia (Col) ecotype had similar phenotypes, and two lines (CFM5088 and CFM5092) were selected for further study. In addition to the phenotypes described, 35S::FUL plants flowered early, produced terminal flowers, and showed a threefold increase in seed weight.
6. S. J. Liljegren et al., Nature 404, 766 (2000).
7. Microscopy, tissue fixation, and histology were as described (2, 6). Plastic sections (1 μm) are shown in Fig. 1, C and D, and paraplast sections (8 or 10 μm) are shown elsewhere.
8. B. Savidge, S. D. Rounsley, M. F. Yanofsky, Plant Cell 7, 721 (1995).
9. C. A. Flanagan, Y. Hu, H. Ma, Plant J. 10, 343 (1996).
10. RNA in situ hybridizations of wild-type (Col), ful-2 (15), 35S::FUL, and shp1-1 shp2-1 sections were done as described (15) with the SHP1 (9), SHP2 (8), and FUL (3) probes. 35S::SHP1/2 plants (6) were crossed to ful-1 mutants (2), because a β-glucuronidase (GUS)-containing enhancer trap element inserted in the 5′-untranslated region behaves as an accurate reporter of FUL expression in ful-1/+ plants. GUS assays of 35S::SHP1/2 ful-1/+ fruit were as described (6).
11. C. Ferrándiz, S. Liljegren, M. Yanofsky, data not shown.
12. shp1 shp2 ful-1 and shp1 shp2 ful-2 fruit were compared with ful-1 and ful-2 fruit, respectively. Fruit lengths (in millimeters; n = 30) at stage 18 were as follows: wild type (Landsberg erecta), 14.2 ± 0.4; ful-1, 3.9 ± 0.1; shp1 shp2 ful-1, 4.1 ± 0.2; wild type (Col), 16.5 ± 0.5; ful-2, 5.6 ± 0.5; shp1 shp2 ful-2, 7.2 ± 0.5. Valve tears were present in 91.0% of ful-1, 2.5% of shp1 shp2 ful-1, and 0% of wild-type fruit (n ≥ 79, stages 17 to 19). Stomata were also observed in shp1 shp2 ful-2 fruit valves.
13. V. Sundaresan et al., Genes Dev. 9, 1797 (1995).
14. Plants carrying the GT140 marker were crossed to ful-2, shp1 shp2 ful-2, and 35S::FUL plants, and GUS assays were as described (6).
15. C. Ferrándiz, Q. Gu, R. Martienssen, M. F. Yanofsky, Development 127, 725 (2000).
16. We thank A. Chen, S. Guimil, T. Kawashiwa, T. Khammungkhune, T. Kuhlmann, and C. Wiley for technical assistance. Supported by grants from the National Science Foundation.