A covalent tandem dimer of the mitochondrial ADP/ATP carrier is functional in vivo

Biochimica et Biophysica Acta - Bioenergetics

Volumn 1457, Issue 1-2, 2000, Pages 81-93

  Trézéguet, Véronique ^a   Le Saux, Agnès ^a   David, Claudine ^a   Gourdet, Céline ^a   Fiore, Christelle ^b   Dianoux, Anne Christine ^b   Brandolin, Gérard ^b   Lauquin, Guy J M ^a  

^a Institut de Biochimie et Genetique Cellulaires   (France)

^b CEA GRENOBLE   (France)

Author keywords

ADP ATP carrier; Covalent tandem dimer; Saccharomyces cerevisiae; Subunit stoichiometry; Topography

Indexed keywords

ADENINE NUCLEOTIDE TRANSLOCASE; ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; DIMER; MONOMER;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL COMPARTMENTALIZATION; COVALENT BOND; GENE CONSTRUCT; MITOCHONDRIAL MEMBRANE; NONHUMAN; NUCLEOTIDE TRANSPORT; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE;

AMINO ACID SEQUENCE; CLONING, MOLECULAR; DIMERIZATION; FUNGAL PROTEINS; INTRACELLULAR MEMBRANES; MITOCHONDRIA; MITOCHONDRIAL ADP, ATP TRANSLOCASES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEAR PROTEINS; PLASMIDS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

SACCHAROMYCES CEREVISIAE;

EID: 0039742269     PISSN: 00052728     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0005-2728(99)00115-2     Document Type: Article

References (43)

1. Capobianco L., Brandolin G., Palmieri F. Transmembrane topography of the mitochondrial phosphate carrier explored by peptide-specific antibodies and enzymatic digestion. Biochemistry. 39:1991;4963-4969.
2. Capobianco L., Bisaccia F., Michel A., Sluse F.E., Palmieri F. The N- and C-termini of the tricarboxylate carrier are exposed to the cytoplasmic side of the inner mitochondrial membrane. FEBS Lett. 357:1995;297-300.
3. Eckerskorn C., Klingenberg M. In the uncoupling protein from brown adipose tissue the C-terminus protrudes to the C-side of the membrane, as shown by tryptic cleavage. FEBS Lett. 226:1987;166-170.
4. 32P]adenosine diphosphate. Biochemistry. 27:1988;5141-5149.
5. Brandolin G., Boulay F., Dalbon P., Vignais P.V. Orientation of the N-terminal region of the membrane bound ADP/ATP carrier explored by antipeptidases and arginine-specific endoproteases. Evidence that the accessibility of the N-terminal residues depends on the conformational state of the carrier. Biochemistry. 28:1989;1093-1100.
6. Hackenberg H., Klingenberg M. Molecular mass and hydrodynamic parameters of the adenosine 5′-diphosphate-adenosine 5′-triphosphate carrier in Triton X-100. Biochemistry. 19:1980;548-555.
7. Block M., Zaccaï G., Lauquin G.J.-M., Vignais P.V. Small angle neutron scattering of the mitochondrial ADP/ATP carrier protein in detergent. Biochem. Biophys. Res. Commun. 109:1982;471-477.
8. Klingenberg M., Riccio P., Aquila H. Isolation of ADP/ATP carrier as the carboxyatractylate protein complex from mitochondria. Biochim. Biophys. Acta. 503:1978;193-210.
9. P.V. Vignais, M.R. Block, F. Boulay, G. Brandolin, G.J.-M. Lauquin, Molecular aspects of structure relationships in mitochondrial adenine nucleotide carrier, in: G. Bengha (Ed.), Structure and Properties of Cell Membranes, vol. II, CRC Press, Boca Raton, FL, 1985, pp. 139-179.
10. Majima E., Ikawa K., Takeda M., Hashimoto M., Shinohara Y., Terada H. Translocation of loops regulates transport activity of mitochondrial ADP/ATP carrier as deduced from formation of a specific intermolecular disulfide bridge catalyzed by copper-o-phenanthroline. J. Biol. Chem. 270:1995;29548-29554.
11. Block M., Vignais P.V. Substrate-site interactions in the membrane-bound adenine-nucleotide carrier as disclosed by ADP and ATP analogs. Biochim. Biophys. Acta. 767:1984;369-376.
12. Dupont Y., Brandolin G., Vignais P.V. Exploration of the nucleotide binding sites of the isolated ADP/ATP carrier protein from Beef heart mitochondria. 1. Probing of the nucleotide sites by naphthoyl-ATP, a fluorescent nontransportable analogue of ATP. Biochemistry. 21:1982;6343-6347.
13. Brandolin G., Dupont Y., Vignais P.V. Exploration of the nucleotide binding sites of the isolated ADP/ATP carrier protein from Beef heart mitochondria. 2. Probing of the nucleotide sites by formicin triphosphate, a fluorescent transportable analogue of ATP. Biochemistry. 21:1982;6348-6353.
14. Drgon T., Sabová L., Nelson N., Kolarov J. ADP/ATP translocator is essential only for anaerobic growth of yeast Saccharomyces cerevisiae. FEBS Lett. 289:1991;159-162.
15. V. Trézéguet, A. Le Saux, G.J.-M. Lauquin, Oligomeric structure of the yeast mitochondrial adenine nucleotide carrier, Yeast 11, Special issue 17th Int. Conf. on Yeast Genetics and Molecular Biology, John Wiley and Sons, New York, 1995 pp. 12-40B.
16. Brandolin G., Le Saux A., Trézéguet V., Vignais P.V., Lauquin G.J.-M. Biochemical characterisation of the isolated Anc2 adenine nucleotide carrier from Saccharomyces cerevisiae mitochondria. Biochem. Biophys. Res. Commun. 192:1993;143-150.
17. F. Sherman, G.R. Fink, C.W. Lawrence, Methods in Yeast Genetics, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1983.
18. Sikorski R.S., Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics. 122:1989;19-27.
19. Brandolin G., Meyer C., Defaye G., Vignais P.M., Vignais P.V. Partial purification of an atractyloside binding protein from mitochondria. FEBS Lett. 46:1974;149-153.
20. Morrison A. Freezing of competent cells. J. Bacteriol. 132:1977;349.
21. Gietz D., St Jean R., Woods R.A., Schiestl R.H. Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res. 20:1992;1425.
22. Holmes D.S., Quigley M. A rapid boiling method for the preparation of bacterial plasmids. Anal. Biochem. 114:1981;193-197.
23. J. Sambrook, E.F. Fritsch, T. Maniatis, Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989.
24. Daum G., Böhni P., Schatz G. Import of proteins into mitochondria. J. Biol. Chem. 257:1982;13028-13033.
25. Le Saux A., Roux P., Trézéguet V., Fiore C., Schwimmer C., Dianoux A.C., Vignais P.V., Brandolin G., Lauquin G.J.-M. Conformational changes of the yeast mitochondrial ADP/ATP carrier studied through its intrinsic fluorescence. 1. The tryptophanyl residues of the carrier can be mutated without impairing protein activity. Biochemistry. 35:1996;16116-16124.
26. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 277:1970;680-685.
27. Kolarov J., Kolarova N., Nelson N. A third ADP/ATP translocator gene in yeast. J. Biol. Chem. 265:1990;12711-12716.
28. Lawson J.E., Gawaz M., Klingenberg M., Douglas M.G. Structure-function studies of adenine nucleotide transport in mitochondria. I. Construction and genetic analysis of yeast mutants encoding the ADP/ATP carrier protein of mitochondria. J. Biol. Chem. 265:1990;14195-14201.
29. Newman M.J., Foster D.L., Wilson T.H., Kaback R.H. Purification and reconstitution of functional lactose carrier from Escherichia coli. J. Biol. Chem. 256:1981;11804-11808.
30. Bibi E., Kaback R.H. In vivo expression of the lacY gene in two segments leads to functional lac permease. Proc. Natl. Acad. Sci. USA. 87:1990;4325-4329.
31. Roux P., Le Saux A., Trézéguet V., Fiore C., Schwimmer C., Dianoux A.C., Vignais P.V., Lauquin G.J.-M., Brandolin G. Conformational changes of the yeast mitochondrial ADP/ATP carrier studied through its intrinsic fluorescence. 2. Assignment of tryptophanyl residues of the carrier to the responses to specific ligands. Biochemistry. 35:1996;16125-16131.
32. Brdiczka D., Schumacher D. Iodination of peripheral mitochondrial membrane proteins in correlation to the functional state of the ADP/ATP carrier. Biochem. Biophys. Res. Commun. 73:1976;823-832.
33. Boulay F., Vignais P.V. Localization of the N-ethylmaleimide reactive cysteine in the beef heart mitochondria ADP/ATP carrier protein. Biochemistry. 23:1984;4807-4812.
34. Bogner W., Aquila H., Klingenberg M. The transmembrane arrangement of the ADP/ATP carrier as elucidated by the lysine reagent pyridoxal-5-phosphate. Eur. J. Biochem. 161:1986;611-620.
35. Marty I., Brandolin G., Vignais P.V. Topography of the membrane-bound ADP/ATP carrier assessed by enzymatic proteolysis. Biochemistry. 31:1992;4058-4065.
36. Duyckaerts C., Sluse-Goffart C.M., Fux J.-P., Sluse F.E., Liebecq C. Kinetic mechanism of the exchanges catalysed by the adenine-nucleotide carrier. Eur. J. Biochem. 106:1980;1-6.
37. Ludewig U., Pusch M., Jentsch T.J. Two physically distinct pores in the dimeric ClC-0 chloride channel. Nature. 383:1996;340-343.
38. Schroers A., Burkovski A., Wohlrab H., Krämer R. The phosphate carrier from yeast mitochondria. Dimerization is a prerequisite for function. J. Biol. Chem. 273:1998;14269-14276.
39. Kovác L., Lachowicz T.M., Slonimski P.P. Biochemical genetics of oxidative phosphorylation. Science. 158:1967;1564-1567.
40. Tytgat J., Hess P. Evidence for cooperative interactions in potassium channel gating. Science. 359:1992;420-423.
41. Kyte J., Doolittle R.F. A simple method for displaying the hydrophobic character of a protein. J. Mol. Biol. 157:1982;105-132.
42. Saraste M., Walker J.E. Internal sequence repeats and the path of polypeptide in mitochondrial ADP/ATP translocase. FEBS Lett. 144:1982;250-254.
43. Müller V., Basset G., Nelson D.R., Klingenberg M. Probing the role of positive residues in the ADP/ATP carrier from yeast. The effect of six arginine mutations on oxidative phosphorylation and AAC expression. Biochemistry. 35:1996;16132-16143.