Organelle purification and selective permeabilisation of the plasma membrane: Two different approaches to study vacuoles of the filamentous fungus Ashbya gossypii

FEMS Microbiology Letters

Volumn 167, Issue 2, 1998, Pages 209-214

  Förster, Carola ^a   Marienfeld, Stephan ^b   Wilhelm, Ralf ^a   Krämer, Reinhard ^a  

^a FORSCHUNGSZENTRUM JÜLICH   (Germany)

^b LEIBNIZ UNIVERSITY HANNOVER   (Germany)

Author keywords

Ashbya gossypii; Filamentous fungus; Isolation; Membrane; Permeabilization; Vacuole

Indexed keywords

ARTICLE; CELL MEMBRANE; CELL ORGANELLE; CELL VACUOLE; FUNGUS; MEMBRANE PERMEABILITY; NONHUMAN; PRIORITY JOURNAL; SOLUBILIZATION;

ACRIDINE ORANGE; ADENOSINE TRIPHOSPHATE; AMINO ACIDS; CELL FRACTIONATION; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; COLORING AGENTS; DIGITONIN; HYDROGEN-ION CONCENTRATION; INTRACELLULAR MEMBRANES; PROTOPLASTS; SACCHAROMYCETALES; VACUOLES;

EREMOTHECIUM GOSSYPII; FUNGI;

EID: 0038851400     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(98)00377-2     Document Type: Article

References (14)

1. Zinser, E. and Daum, G. (1995) Isolation and biochemical characterization of organelles from the yeast, Saccharomyces cerevisiae. Yeast 11, 493-536.
2. Klionsky, D.J., Herman, P.K. and Emr, S.D. (1990) The fungal vacuole: composition, function, and biogenesis. Microbiol. Rev. 54, 266-292.
3. Messenguy, F., Colin, D. and ten Have, J. (1980) Regulation of compartmentation of amino pools in Saccharomyces cervisiae and its effects on metabolic control. Eur. J. Biochem. 108, 439-447.
4. Roos, W., Schulze, R. and Steighardt, J. (1997) Dynamic compartmentation of vacuolar amino acids in Penicillium cyclopium. J. Biol. Chem. 272, 15894-15855.
5. Guillermond, P. (1928) Recherches sur quelques Ascomycetes inferieurs isoles de la stigmatomycose des graines de cotonnier. Essay sur la phylogenie des Ascomycetes. Rev. Gen. Bot. 40, 328-342, 397-414, 474-485, 555-574, 606-624.
6. Schmidt, G., Stahmann, K.P., Kaesler, B. and Sahm, H. (1996) Correlation of isocitrate lyase activity and riboflavin formation in the riboflavin overproducer Ashbya gossypii. Microbiology 142, 419-426.
7. Schimz, K.L., Rütten, B. and Tretter, M. (1981) Determination of adenosine nucleotides with luciferin/luciferase from crude firefly lantern extract on a bioluminescence analyser. Adv. Biotechnol. 1, 457-462.
8. Ram, D., Tulsiani, P. and Tonsler, O. (1978) Alpha-D-mannosidase from Golgi membranes of rat liver. Methods Enzymol. 50, 500-505.
9. Peterson, C.A. (1979) Selective vital staining of companion cells of potato tuber and parsnip root with neutral red. Stain Technol. 54, 135-139.
10. Bowman, E.J. and Bowman, B.J. (1982) Identification and properties of an ATPase in vacuolar membranes of Neurospora crassa. J. Bacteriol. 151, 1326-1337.
11. Laouar, L., Mulligan, B.J. and Lowe, K.C. (1992) Yeast permeabilization with surfactants. Biotechnol. Lett. 14, 719-720.
12. Huber-Wälchli, V. and Wiemken, A. (1979) Differential extraction of soluble pools from the cytosol and the vacuoles of yeast (Candida utilis) using DEAE-dextran. Arch. Microbiol. 120, 141-149.
13. Cononico, P.G. and Bird J.W.C. (1969) The use of acridine orange as a lysosomal marker in rat skeletal muscle. J. Cell Biol. 43, 367-371.
14. Okros, I. (1968) Digitonin reaction in electron microscopy. Histochemie 13, 91-96.