Development of microsatellite markers for Primula sieboldii using degenerate oligonucleotide-primed PCR-amplified DNA

Molecular Ecology Notes

Volumn 1, Issue 1-2, 2001, Pages 22-24

  Isagi, Y ^a   Honjo, M ^b   Washitani, I ^c  

^a HIROSHIMA UNIVERSITY   (Japan)

^b UNIVERSITY OF TSUKUBA   (Japan)

^c UNIVERSITY OF TOKYO   (Japan)

Author keywords

DOP PCR; Microsatellites; Primula; Single locus DNA markers

Indexed keywords

EID: 0038822451     PISSN: 14718278     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-8278.2000.00009.x     Document Type: Article

References (8)

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3. Milligan B (1992) Plant DNA isolation. In: Molecular Genetic Analysis of Populations: a Practical Approach (ed. Hoelzel AR), pp. 59-88. IRL Press, Oxford.
4. Raymond M & Rousset R (1995) GENEPOP (Version 1.2): population genetics software for exact tests and ecumenicism. Journal of Heredity, 86, 248-249.
5. Telenius H, Carter NP, Bebb CE, Nordenskjold M, Ponder BAJ & Tunnacliffe A (1992) Degenerate oligonucleotide-primed PCR: general amplification of target DNA by a single degenerate primer. Genomics, 13, 718-725.
6. Washitani I (1996) Predicted genetic consequences of strong fertility selection due to pollinator loss in an isolated population of Primula sieboldii. Conservation Biology, 10, 59-64.
7. Washitani I, Namai H, Osawa R & Niwa M (1991) Species biology of Primula sieboldii for the conservation of its lowland-habitat population: I. Inter-clonal variations in the flowering phenology, pollen load and female fertility components. Plant Species Biology, 6, 27-37.
8. Washitani I, Osawa R, Namai H & Niwa M (1994) Patterns of female fertility in heterostylous Primula sieboldii under severe pollinator limitation. Journal of Ecology, 82, 571-579.