Cloning and comparative analysis of the venom prepromelittin genes from four wasp species

Acta Genetica Sinica

Volumn 30, Issue 6, 2003, Pages 555-559

  Shi, Wan Jun ^a   Zhang, Su Fang ^a   Zhang, Chuan Xi ^a   Cheng, Jia An ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

Prepromelittin; Sequence comparison; Venom; Vespidae

Indexed keywords

ETHIDIUM BROMIDE; MELITTIN; POLYPEPTIDE; PREPROMELITTIN; PROMELITTIN; SIGNAL PEPTIDE; UNCLASSIFIED DRUG; WASP VENOM;

AMINO ACID SEQUENCE; APIS CERANA; ARTICLE; COMPARATIVE STUDY; DNA SYNTHESIS; DNA VECTOR; ELECTROPHORESIS; GENE; GENETIC ANALYSIS; HONEYBEE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PGEM T EASY VECTOR; PHYLOGENY; POLISTES HEBRAEUS; POLYMERASE CHAIN REACTION; PREPROMELITTIN GENE; PROTEIN STRUCTURE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ISOLATION; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SPECIES DIFFERENCE; STAINING; VESPA MAGNIFICA; VESPA VELUTINA; VESPULA MACULIFRONS; WASP;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ELECTROPHORESIS, AGAR GEL; MELITTEN; MOLECULAR SEQUENCE DATA; PHYLOGENY; PROTEIN PRECURSORS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SEQUENCE HOMOLOGY, NUCLEIC ACID; SPECIES SPECIFICITY; WASPS;

APINAE; APIS; APIS CERANA; APIS MELLIFERA; APOIDEA; HEXAPODA; POLISTES; POLISTES HEBRAEUS; VELUTINA; VESPA; VESPA MAGNIFICA; VESPA VELUTINA NIGRITHORAX; VESPIDAE; VESPULA; VESPULA MACULIFRONS;

EID: 0038787982     PISSN: 03794172     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (10)

1. FANG Zhu, YANG Yu-Lin, ZHOU Bang-Xun. The study and usage of bee venom. Beijing: Science Press, 1988, 128-144.
2. ZHU Wei, WANG Ben-Xiang, ZHU Xun. The progress in the study of melittin. J N Bethune Univ Med Sci, 2001, 27(2): 212-214.
3. ZHOU Shu-Ping, LI Ying. The progress in the study of honey bee melittin. Study of biochimica et biophysica, 1986, 4(1): 31-36.
4. Vlasak R, Ungen U C, Gunther K, Anna-Maria F. Nucleotide sequence of cloned cDNA coding for honeybee prepromelittin. Eur J Biochem, 1983, 135(1): 123-126.
5. Matsuzaki K, Yoneyama S, Miyajima K. Pore formation and translocation of melittin. Biophysical Journal, 1997, 73(2): 831-838.
6. Liu P, Davis P, Liu H, krishnan T R. Evaluation of cytotoxicity and absorption enhacing effects of melittin-a novel absorption enhancer. European Journal of Parmaceutics and Biopharmaceutics, 1999, 48 (1): 85-87.
7. Lazarev V N, Parfenova T M, Gularyan S K, Misyurina O Yu, Akopian T A, Govorun V M. Induced expression of melittin, an antimicrobial peptide, inhibits infection by Chlamydia trachomatis and Mycoplasma hominis in a HeLa cell line. International Journal of Antimicrobial Agents, 2002, 19(2): 133-137.
8. LI Ji-Zhou, CHEN Zhong-Bing, CAI Qing-Nian, ZHANG Qing-Wen. Cloning of melittin cDNA from honey bees. Journal of Agricultural Biotechnology, 1997, 5(2): 162-167.
9. WANG Guan-Lin, LI Da-Li, FANG Hong-Jun. Site-directed mutagenesis of melittin gene and its expression in Escherichia coli. Acta Genetica Sinica, 2000, 27(2): 176-182.
10. WANG Guan-Lin, LI Da-Li, FANG Hong-Jun. Cloning of promelittin cDNA and its expression in Escherichia coli. Acta Microbiologica Sinica, 2001, 41(2): 181-185.