Combination of SEC/MALS experimental procedures and theoretical analysis for studying the solution properties of macromolecules

Journal of Biochemical and Biophysical Methods

Volumn 56, Issue 1-3, 2003, Pages 95-116

  Tarazona, M Pilar ^a   Saiz, Enrique ^a  

^a UNIVERSITY OF ALCALÁ   (Spain)

Author keywords

Conformational properties; Molecular dimension; Molecular dynamics; Poly(N vinylcarbazole); Polyvinylpyrrolidone; Size exclusion chromatography; Solution properties

Indexed keywords

CARBAZOLE DERIVATIVE; FREE RADICAL; ORGANIC SOLVENT; POLY(N VINYLCARBAZOLE); POLYMER; POVIDONE; SODIUM NITRATE; UNCLASSIFIED DRUG; WATER;

ARTICLE; CALIBRATION; CONCENTRATION (PARAMETERS); CONFORMATION; DISPERSION; GEL PERMEATION CHROMATOGRAPHY; ISOMERISM; LIGHT SCATTERING; MACROMOLECULE; MATHEMATICAL ANALYSIS; MOLECULAR DYNAMICS; MOLECULAR WEIGHT; PARAMETER; POLYMERIZATION; PRIORITY JOURNAL; REFRACTION INDEX; TEMPERATURE; THEORETICAL MODEL; THEORETICAL STUDY;

EID: 0038771207     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-022X(03)00075-7     Document Type: Article

References (37)

1. Trathnigg B. Determination of MWD and chemical composition of polymers by chromatographic techniques. Prog. Polym. Sci. 20:1995;615-650.
2. Potschka M., Dubin P.L. Strategies in size exclusion chromatography. 1996;American Chemical Society, Washington, DC.
3. Barth H.G., Boyes B.E., Jackson C. Size exclusion chromatography and related separation techniques. Anal. Chem. 70:1998;251R-278R.
4. Wyatt P.J. Light scattering and the absolute characterization of macromolecules. Anal. Chim. Acta. 271:1993;1-40.
5. Wyatt P.J. Mean square radius of molecules and secondary instrumental broadening. J. Chromatogr. 648:1993;27-32.
6. Laguna M.T.R., Medrano R., Plana M.P., Tarazona M.P. Polymer characterization by size-exclusion chromatography with multiple detection. J. Chromatogr., A. 919:2001;13-19.
7. Flory P.J. Statistical mechanics of chain molecules. 1969;Wiley, New York.
8. g) states are always in unperturbed conditions because inter- and intramolecular interactions cancel each other out.
9. Yamakawa H. Modern theory of polymer solutions. 1971;Harper & Row, New York.
10. Búrdalo J., Medrano R., Saiz E., Tarazona M.P. A simple method to determine unperturbed dimensions of polymers using size exclusion chromatography and multiangle light scattering. Polymer. 41:2000;1615-1620.
11. Gallego J., Pérez-Foullerat D., Mendicuti F., Mattice W.L. Configuration conducive to the formations of intramolecular excimers in poly(N-vinylcarbazole) and its copolymers. J. Polym. Sci., Part B: Polym. Phys. 39:2001;1272-1276.
12. Huglin M.B. Light scattering from polymer solutions. 1972;Academic Press, London.
13. De Gennes P.G. Scaling concepts in polymer physics. 1979;Cornell Univ. Press, Ithaca, NY.
14. Stockmayer W.H., Fixman M. On the estimation of unperturbed dimensions from intrinsic viscosities. J. Polym. Sci. C. 1:1963;137-141.
15. Fixman M. Excluded volume in polymer chains. J. Chem. Phys. 23:1955;1656-1659.
16. Kurata M., Stockmayer W.H., Roig A. Excluded-volume effect of linear polymer molecules. J. Chem. Phys. 33:1960;151.
17. Laguna M.T.R., Saiz E., Tarazona M.P. Solution properties of poly[diphenoxy phosphazene] below the θ temperature obtained by SEC/MALLS. Polymer. 41:2000;7993-8000.
18. Laguna M.T.R., Tarazona M.P. Solution properties of poly[bis(piperidino) phosphazene] by size exclusion chromatography and multiangle light scattering. Polymer. 42:2001;1751-1756.
19. Brandrup J., Immergut E.H., Grulke E.A. Polymer handbook. 4th ed. 1999;Wiley, New York.
20. Tarazona M.P., Saiz E., Gargallo L., Radic D. Conformational characteristics of poly(vinylpyrrolidone). Solvent-dependence of the chain dimensions. Makromol. Chem., Theory Simul. 2:1993;697-710.
21. Mattice W.L., Suter U.W. Conformational theory of large molecules. The rotational isomeric state model in macromolecular systems. 1994;Wiley-Interscience, New York.
22. Kuhn W. The relation between molecular size, statistical molecular shape and elastic properties of highly polymerized substances. Kolloid Z. 76:1936;258-271 Molecular configuration and crystalline orientation as causes of rubber-like elasticity. Kolloid Z. 87:1939;3-12.
23. 2, i.e., the length of the chain when all bond angles are forced to open to 180°. The term sin(θ/2) in Eq. (15) is then unity, and the values obtained for N and L are different, although Eq. (14) still holds true.
24. Porod G. The mean separation of chain ends in threadlike molecules. Monatsh. Chem. 80:1949;251-255.
25. Kratky O., Porod G. X-ray investigations of dissolved chain molecules. Rec. Trav. Chim. 68:1949;1106-1122.
26. Volkenstein M.V. Configurational statistics of polymeric chains. 1963;Interscience, New York.
27. Birshtein T.M., Ptitsyn O.B. Conformations of macromolecules. 1966;Interscience, New York.
28. Nagai K. Non-Gaussian character of real polymer chains. J. Chem. Phys. 38:1963;924-933.
29. Flory P.J. Foundations of rotational isomeric state theory and general methods for generating configurational averages. Macromolecules. 7:1974;381-392.
30. Riande E., Saiz E. Dipole moments and birefringence of polymers. 1992;Prentice-Hall, New York.
31. Sundararajan P.R., Flory P.J. Configurational characteristics of poly(methyl methacrylate). J. Am. Chem. Soc. 96:1974;5025.
32. Laguna M.T.R., Gallego J., Mendicuti F., Saiz E., Tarazona M.P. Solution properties of poly(N-vinylcarbazole) and its copolymers with methyl methacrylate. Macromolecules. 35:2002;7782-7790.
33. Abe A., Kobayashi H., Kawamura T.M., Date M., Uryu T., Matsuzaki K. Statistical properties of the N-vinylcarbazole chain as estimated from NMR analysis. Macromolecules. 21:1988;3414.
34. T.R. Forester, W. Smith, DL_POLY (Ver. 2.10). Daresbury Laboratory, Daresbury, Warrington WA4 4AD, England.
35. Allen M.P., Tildesley D.J. Computer simulation of liquids. 1987;Clarendon, Oxford.
36. Cornell W.D., Cieplak P., Bayly C.L., Gould I.R., Merz K.M., Ferguson D.M.D.et al. A second generation force field for the simulation of proteins, nucleic acids, and organic molecules. J. Am. Chem. Soc. 117:1995;5179-5197.
37. MOPAC, Quantum Chemistry Program Exchange, Department of Chemistry, Indiana University, Bloomington, IN.