-
1
-
-
0034714499
-
Gene expression profiling: Monitoring transcription and translation products using DNA microarrays and proteomics
-
Celis J.E., Kruhoffer M., Gromove I., Frederiksen C., Ostergaard M., Thykjaer T., Gromova P., Yu J., Palsdottir H., Magnusson N., Ornoft T.F. Gene expression profiling: monitoring transcription and translation products using DNA microarrays and proteomics. FEBS Lett. 480:2000;2-16.
-
(2000)
FEBS Lett.
, vol.480
, pp. 2-16
-
-
Celis, J.E.1
Kruhoffer, M.2
Gromove, I.3
Frederiksen, C.4
Ostergaard, M.5
Thykjaer, T.6
Gromova, P.7
Yu, J.8
Palsdottir, H.9
Magnusson, N.10
Ornoft, T.F.11
-
2
-
-
0036007687
-
Metabolomics - the link between genotypes and phenotypes
-
A comprehensive review of technologies utilised in metabolite analysis giving rigid definitions of the broad types of metabolite analysis from target analyses to fingerprinting. This review also provides a useful introduction to contemporary data-mining techniques and the metabolic modelling of metabolism.
-
Fiehn O. Metabolomics. - the link between genotypes and phenotypes Plant Mol. Biol. 48:2002;155-171 A comprehensive review of technologies utilised in metabolite analysis giving rigid definitions of the broad types of metabolite analysis from target analyses to fingerprinting. This review also provides a useful introduction to contemporary data-mining techniques and the metabolic modelling of metabolism.
-
(2002)
Plant Mol. Biol.
, vol.48
, pp. 155-171
-
-
Fiehn, O.1
-
3
-
-
0001317950
-
Rapid fractionation of wheat leaf protoplasts using membrane filtration - the determination of metabolite levels in the chloroplasts, cytosol, and mitochondria
-
Lilley R.M., Stitt M., Mader G., Heldt H.W. Rapid fractionation of wheat leaf protoplasts using membrane filtration. - the determination of metabolite levels in the chloroplasts, cytosol, and mitochondria Plant Physiol. 70:1982;965-970.
-
(1982)
Plant Physiol.
, vol.70
, pp. 965-970
-
-
Lilley, R.M.1
Stitt, M.2
Mader, G.3
Heldt, H.W.4
-
4
-
-
84989735714
-
2 fixation. 1. Secondary kinetics during photosynthetic induction are related to a delayed activation of sucrose synthesis
-
2 fixation. 1. Secondary kinetics during photosynthetic induction are related to a delayed activation of sucrose synthesis. J. Plant Physiol. 133:1988;129-137.
-
(1988)
J. Plant Physiol.
, vol.133
, pp. 129-137
-
-
Stitt, M.1
Grosse, H.2
-
5
-
-
0033765590
-
Reciprocal diurnal changes of phosphoenolpyruvate carboxylase expression and cytosolic pyruvate kinase, citrate synthase and NADP-isocitrate dehydrogenase expression regulate organic acid metabolism during nitrate assimilation in tobacco leaves
-
This paper presents a multilevel analysis of the diurnal changes in transcripts and the activities of several enzymes involved in nitrate and ammonium assimilation and organic acid metabolism. These changes are then related to the variations of a range of metabolites in primary carbon and nitrogen metabolism in wild-type tobacco, mutants with decreased nitrate reductase activity, and nitrate-limited plants. The results uncover an integrated network in which the genes that are required during nitrate assimilation fall into two coordinated groups both showing marked diurnal changes in expression and activity. The two groups move almost 180° out-of-phase to each other, and result in significant changes in metabolic fluxes as expression changes during the cycle
-
Scheible W.R., Krapp A., Stitt M. Reciprocal diurnal changes of phosphoenolpyruvate carboxylase expression and cytosolic pyruvate kinase, citrate synthase and NADP-isocitrate dehydrogenase expression regulate organic acid metabolism during nitrate assimilation in tobacco leaves. Plant Cell Environ. 23:2000;1155-1167 This paper presents a multilevel analysis of the diurnal changes in transcripts and the activities of several enzymes involved in nitrate and ammonium assimilation and organic acid metabolism. These changes are then related to the variations of a range of metabolites in primary carbon and nitrogen metabolism in wild-type tobacco, mutants with decreased nitrate reductase activity, and nitrate-limited plants. The results uncover an integrated network in which the genes that are required during nitrate assimilation fall into two coordinated groups both showing marked diurnal changes in expression and activity. The two groups move almost 180° out-of-phase to each other, and result in significant changes in metabolic fluxes as expression changes during the cycle. These changes are driven by nitrate assimilation and reflect changes in prioritisation shifts between nitrate assimilation and pH regulation in the early part of the light period and use of the reduced nitrogen later in the cycle.
-
(2000)
Plant Cell Environ.
, vol.23
, pp. 1155-1167
-
-
Scheible, W.R.1
Krapp, A.2
Stitt, M.3
-
6
-
-
0036830074
-
Diurnal changes in the expression of glutamate dehydrogenase and nitrate reductase are involved in the C/N balance of tobacco source leaves
-
Masclaux-Daubresse C., Valadier M.H., Carrayol E., Reisdorf-Cren M., Hirel B. Diurnal changes in the expression of glutamate dehydrogenase and nitrate reductase are involved in the C/N balance of tobacco source leaves. Plant Cell Environ. 25:2002;1451-1462.
-
(2002)
Plant Cell Environ.
, vol.25
, pp. 1451-1462
-
-
Masclaux-Daubresse, C.1
Valadier, M.H.2
Carrayol, E.3
Reisdorf-Cren, M.4
Hirel, B.5
-
7
-
-
0036017869
-
Decreased Rubisco activity leads to dramatic changes of nitrate metabolism, amino acid metabolism and the levels of phenylpropanoids and nicotine in tobacco antisense RBCS transformants
-
This study of the nitrogen and secondary metabolism of antisense RBCS tobacco plants exhibiting a lesion in photosynthetic carbon metabolism revealed that the decrease in sugar levels (following inhibition of Rubisco) leads to a general inhibition of nitrogen metabolism, and dramatic changes in the levels of secondary metabolites. Moreover, changes of the amino acid: sugar ratio were shown to be accompanied by changes of the nicotine:chlorogenic acid ratio.
-
Matt P., Krapp A., Haake V., Mock H.P., Stitt M. Decreased Rubisco activity leads to dramatic changes of nitrate metabolism, amino acid metabolism and the levels of phenylpropanoids and nicotine in tobacco antisense RBCS transformants. Plant J. 30:2002;663-677 This study of the nitrogen and secondary metabolism of antisense RBCS tobacco plants exhibiting a lesion in photosynthetic carbon metabolism revealed that the decrease in sugar levels (following inhibition of Rubisco) leads to a general inhibition of nitrogen metabolism, and dramatic changes in the levels of secondary metabolites. Moreover, changes of the amino acid: sugar ratio were shown to be accompanied by changes of the nicotine:chlorogenic acid ratio.
-
(2002)
Plant J.
, vol.30
, pp. 663-677
-
-
Matt, P.1
Krapp, A.2
Haake, V.3
Mock, H.P.4
Stitt, M.5
-
8
-
-
0036068990
-
How does photorespiration modulate leaf amino acid contents? A dual approach through modeling and metabolite analysis
-
This work demonstrated the quantitative impact of photorespiration on leaf amino acid contents in wheat and potato leaves. The amino acid concentrations were measured directly and the photorespiratory flux calculated following a simple model. Interesting patterns emerged between the levels of amino acids and flux. The authors use model-based analyses to reach the conclusion that primary nitrogen assimilation has a potentially marked impact on leaf glutamine, even against a background of high rates of photorespiratory ammonia recycling.
-
Novistskaya L., Trevanion S.J., Driscoll S., Foyer C.H., Noctor G. How does photorespiration modulate leaf amino acid contents? A dual approach through modeling and metabolite analysis. Plant Cell Environ. 25:2002;821-835 This work demonstrated the quantitative impact of photorespiration on leaf amino acid contents in wheat and potato leaves. The amino acid concentrations were measured directly and the photorespiratory flux calculated following a simple model. Interesting patterns emerged between the levels of amino acids and flux. The authors use model-based analyses to reach the conclusion that primary nitrogen assimilation has a potentially marked impact on leaf glutamine, even against a background of high rates of photorespiratory ammonia recycling.
-
(2002)
Plant Cell Environ.
, vol.25
, pp. 821-835
-
-
Novistskaya, L.1
Trevanion, S.J.2
Driscoll, S.3
Foyer, C.H.4
Noctor, G.5
-
9
-
-
0036325472
-
3- supply on N metabolism of potato plants (Solanum tuberosum L.) with special focus on the tubers
-
3- supply on N metabolism of potato plants (Solanum tuberosum L.) with special focus on the tubers. Plant Cell Environ. 25:2002;999-1009.
-
(2002)
Plant Cell Environ.
, vol.25
, pp. 999-1009
-
-
Mäck, G.1
Schjoerring, J.K.2
-
10
-
-
0035999896
-
Sensitive and high-throughput metabolite assays for inorganic pyrophosphate, ADPGlc, nucleotide phosphates, and glycolytic intermediates based on a novel enzymic cycling system
-
This paper describes the development of a set of assays based on a novel enzymatic cycling system between glycerol-3-phosphate dehydrogenase and glycerol-3-phosphate oxidase. The assays allowed the rapid and sensitive detection of metabolites that are typically difficult to measure via MS-based methodologies. This method, therefore, represents an enabling technology for the study of central metabolism in plants and will provide a useful tool for addressing questions on carbon-nitrogen metabolism.
-
Gibon Y., Vigeolas H., Tiessen A., Geigenberger P., Stitt M. Sensitive and high-throughput metabolite assays for inorganic pyrophosphate, ADPGlc, nucleotide phosphates, and glycolytic intermediates based on a novel enzymic cycling system. Plant J. 30:2002;221-235 This paper describes the development of a set of assays based on a novel enzymatic cycling system between glycerol-3-phosphate dehydrogenase and glycerol-3-phosphate oxidase. The assays allowed the rapid and sensitive detection of metabolites that are typically difficult to measure via MS-based methodologies. This method, therefore, represents an enabling technology for the study of central metabolism in plants and will provide a useful tool for addressing questions on carbon-nitrogen metabolism.
-
(2002)
Plant J.
, vol.30
, pp. 221-235
-
-
Gibon, Y.1
Vigeolas, H.2
Tiessen, A.3
Geigenberger, P.4
Stitt, M.5
-
11
-
-
0034837512
-
Rapid HPLC screening of jasmonate-induced increases in tobacco alkaloids, phenolics, and diterpene glycosides in Nicotiana attenuata
-
Kinanen M., Oldham N.J., Baldwin I.T. Rapid HPLC screening of jasmonate-induced increases in tobacco alkaloids, phenolics, and diterpene glycosides in Nicotiana attenuata. J. Agric Food Chem. 49:2001;3553-3558.
-
(2001)
J. Agric Food Chem.
, vol.49
, pp. 3553-3558
-
-
Kinanen, M.1
Oldham, N.J.2
Baldwin, I.T.3
-
12
-
-
0034830836
-
Isoprenoid synthesis. Metabolite profiling of peppermint oil gland secretory cells and application to herbicide target analysis
-
Lange B.H., Ketchum R.E.B., Croteau R.B. Isoprenoid synthesis. Metabolite profiling of peppermint oil gland secretory cells and application to herbicide target analysis. Plant Physiol. 127:2001;305-314.
-
(2001)
Plant Physiol.
, vol.127
, pp. 305-314
-
-
Lange, B.H.1
Ketchum, R.E.B.2
Croteau, R.B.3
-
13
-
-
0034282910
-
Metabolite profiling of sesquiterpene lactones from Lactuca species
-
Sessa R.A., Bennett M.H., Lewis M.J., Mansfield J.W., Beale M.H. Metabolite profiling of sesquiterpene lactones from Lactuca species. J. Biol. Chem. 275:2000;26877-26884.
-
(2000)
J. Biol. Chem.
, vol.275
, pp. 26877-26884
-
-
Sessa, R.A.1
Bennett, M.H.2
Lewis, M.J.3
Mansfield, J.W.4
Beale, M.H.5
-
14
-
-
0035043360
-
A systematic approach to biochemical profiling
-
Glassbrook N., Ryals J. A systematic approach to biochemical profiling. Curr. Opin. Plant Biol. 4:2001;186-190.
-
(2001)
Curr. Opin. Plant Biol.
, vol.4
, pp. 186-190
-
-
Glassbrook, N.1
Ryals, J.2
-
15
-
-
0035313629
-
Gene discovery via metabolic profiling
-
Trethewey R.N. Gene discovery via metabolic profiling. Curr Opin Biotechnol. 12:2001;135-138.
-
(2001)
Curr Opin Biotechnol
, vol.12
, pp. 135-138
-
-
Trethewey, R.N.1
-
16
-
-
0037083636
-
Analysis of highly polar compounds of plant origin: Combination of hydrophilic interaction chromatography and electrospray ion trap mass spectroscopy
-
The authors demonstrate the separation and analysis of highly polar compounds in an elegant proof-of-concept study to illustrate how unknown metabolites can be identified by stepwise MS fragmentation of the most intense ions. As the identification of unknown compounds represents a 'bottleneck' in metabolomic analysis, this study and others based on it will prove invaluable in the future development of this subject.
-
Tolstikov V.V., Fiehn O. Analysis of highly polar compounds of plant origin: combination of hydrophilic interaction chromatography and electrospray ion trap mass spectroscopy. Anal. Biochem. 301:2002;298-307 The authors demonstrate the separation and analysis of highly polar compounds in an elegant proof-of-concept study to illustrate how unknown metabolites can be identified by stepwise MS fragmentation of the most intense ions. As the identification of unknown compounds represents a 'bottleneck' in metabolomic analysis, this study and others based on it will prove invaluable in the future development of this subject.
-
(2002)
Anal. Biochem.
, vol.301
, pp. 298-307
-
-
Tolstikov, V.V.1
Fiehn, O.2
-
17
-
-
0033757963
-
Metabolite profiling for plant functional genomics
-
Fiehn O., Kopka J., Dörmann P., Altmann T., Trethewey R.N., Willmitzer L. Metabolite profiling for plant functional genomics. Nat. Biotechnol. 18:2000;1157-1161.
-
(2000)
Nat. Biotechnol.
, vol.18
, pp. 1157-1161
-
-
Fiehn, O.1
Kopka, J.2
Dörmann, P.3
Altmann, T.4
Trethewey, R.N.5
Willmitzer, L.6
-
18
-
-
0035105106
-
Metabolic profiling allows comprehensive phenotyping of genetically or environmentally modified plant systems
-
This study demonstrates that metabolic profiling not only allows the comprehensive phenotyping of plant systems, but also illustrates the possibility of replicating the metabolite complement of genetically modified systems by environmental perturbations. Thus, similarities in systemic responses to diverse manipulations are revealed. Furthermore, for the first time, the authors discuss the possible interpretations of metabolite:metabolite correlations calculated from profiling data.
-
Roessner U., Luedemann A., Brust D., Fiehn O., Linke T., Willmitzer L., Fernie A.R. Metabolic profiling allows comprehensive phenotyping of genetically or environmentally modified plant systems. Plant Cell. 13:2001;11-29 This study demonstrates that metabolic profiling not only allows the comprehensive phenotyping of plant systems, but also illustrates the possibility of replicating the metabolite complement of genetically modified systems by environmental perturbations. Thus, similarities in systemic responses to diverse manipulations are revealed. Furthermore, for the first time, the authors discuss the possible interpretations of metabolite:metabolite correlations calculated from profiling data.
-
(2001)
Plant Cell
, vol.13
, pp. 11-29
-
-
Roessner, U.1
Luedemann, A.2
Brust, D.3
Fiehn, O.4
Linke, T.5
Willmitzer, L.6
Fernie, A.R.7
-
19
-
-
0037138280
-
Metabolic profiling of saponins in Medicago sativa and Medicago trunculata using HPLC coupled to an electrospray ion-trap mass spectrometer
-
Huhman D.V., Sumner L.W. Metabolic profiling of saponins in Medicago sativa and Medicago trunculata using HPLC coupled to an electrospray ion-trap mass spectrometer. Phytochem. 59:2002;347-360.
-
(2002)
Phytochem.
, vol.59
, pp. 347-360
-
-
Huhman, D.V.1
Sumner, L.W.2
-
20
-
-
0035984047
-
Plant metabolomics: The missing link in functional genomics strategies
-
Hall R., Beale M., Fiehn O., Hardy N., Sumner L., Bino R. Plant metabolomics: the missing link in functional genomics strategies. Plant Cell. 14:2002;1437-1440.
-
(2002)
Plant Cell
, vol.14
, pp. 1437-1440
-
-
Hall, R.1
Beale, M.2
Fiehn, O.3
Hardy, N.4
Sumner, L.5
Bino, R.6
-
22
-
-
0033978640
-
Chemical fingerprinting for the evaluation of unintended secondary metabolic changes in transgenic food crops
-
Noteborn H.P.J.M., Lommen A., van der Jagt R.C., Wewseman J.M. Chemical fingerprinting for the evaluation of unintended secondary metabolic changes in transgenic food crops. J. Biotechnol. 77:2000;103-114.
-
(2000)
J. Biotechnol.
, vol.77
, pp. 103-114
-
-
Noteborn, H.P.J.M.1
Lommen, A.2
Van der Jagt, R.C.3
Wewseman, J.M.4
-
23
-
-
0035170619
-
Elevated carbon dioxide increases nitrate uptake and nitrate reductase activity when tobacco is growing on nitrate, but increases ammonium uptake and inhibits nitrate reductase activity when tobacco is growing on ammonium nitrate
-
Matt P., Geiger M., Walch-Liu P., Engels C., Krapp A., Stitt M. Elevated carbon dioxide increases nitrate uptake and nitrate reductase activity when tobacco is growing on nitrate, but increases ammonium uptake and inhibits nitrate reductase activity when tobacco is growing on ammonium nitrate. Plant Cell Environ. 24:2001;1119.
-
(2001)
Plant Cell Environ.
, vol.24
, pp. 1119
-
-
Matt, P.1
Geiger, M.2
Walch-Liu, P.3
Engels, C.4
Krapp, A.5
Stitt, M.6
-
24
-
-
0035134379
-
The immediate cause of the diurnal changes of nitrogen metabolism in leaves of nitrate-replete tobacco: A major imbalance between the rate of nitrate reduction and the rates of nitrate uptake and ammonium metabolism during the first part of the light period
-
•] illustrates the importance of serial sampling and thus of temporal understanding of metabolism. It focuses on transcripts, enzyme activities and metabolite levels in tobacco leaves and nitrate uptake in the tobacco root and includes estimations of the changes in diurnal fluxes of nitrate metabolism. Following this experimental track allowed the authors to build up a complex model of whole-plant nitrogen metabolism in tobacco from the level of transcription to that of metabolic flux during the diurnal cycle.
-
•] illustrates the importance of serial sampling and thus of temporal understanding of metabolism. It focuses on transcripts, enzyme activities and metabolite levels in tobacco leaves and nitrate uptake in the tobacco root and includes estimations of the changes in diurnal fluxes of nitrate metabolism. Following this experimental track allowed the authors to build up a complex model of whole-plant nitrogen metabolism in tobacco from the level of transcription to that of metabolic flux during the diurnal cycle.
-
(2001)
Plant Cell Environ.
, vol.24
, pp. 177-190
-
-
Matt, P.1
Geiger, M.2
Walch-Liu, P.3
Engels, C.4
Krapp, A.5
Stitt, M.6
-
25
-
-
1842369047
-
Tobacco mutants with a decreased number of functional nia genes compensate by modifying the diurnal regulation of transcription, post-translational modification and turnover of nitrate reductase
-
Scheible W.R., Gonzalez-Fontes A., Morcuende R., Lauerer M., Geiger M., Glaab J., Gojon A., Schulze E.D., Stitt M. Tobacco mutants with a decreased number of functional nia genes compensate by modifying the diurnal regulation of transcription, post-translational modification and turnover of nitrate reductase. Planta. 203:1997;304-319.
-
(1997)
Planta
, vol.203
, pp. 304-319
-
-
Scheible, W.R.1
Gonzalez-Fontes, A.2
Morcuende, R.3
Lauerer, M.4
Geiger, M.5
Glaab, J.6
Gojon, A.7
Schulze, E.D.8
Stitt, M.9
-
26
-
-
0001349167
-
Transport of anions in isolated barley vacuoles i. Permeability to anions and evidence for a chloride uptake system
-
Martinoia E., Schramm M.J., Kaiser G., Kaiser W.M., Heber U. Transport of anions in isolated barley vacuoles i. Permeability to anions and evidence for a chloride uptake system. Plant Physiol. 80:1986;895-901.
-
(1986)
Plant Physiol.
, vol.80
, pp. 895-901
-
-
Martinoia, E.1
Schramm, M.J.2
Kaiser, G.3
Kaiser, W.M.4
Heber, U.5
-
27
-
-
0024831970
-
Determination of metabolite levels in specific cells and subcellular compartments of plant leaves
-
Stitt M., Lilley R., Gerhardt R., Heldt H.W. Determination of metabolite levels in specific cells and subcellular compartments of plant leaves. Methods Enzymol. 174:1989;518-552.
-
(1989)
Methods Enzymol.
, vol.174
, pp. 518-552
-
-
Stitt, M.1
Lilley, R.2
Gerhardt, R.3
Heldt, H.W.4
-
28
-
-
0034783268
-
Photorespiratory flux and mitochondrial contribution to energy and redox balance of barley leaf protoplasts in the light and during light-dark transitions
-
In this paper the contribution of mitochondrial oxidation of photorespiratory and respiratory substrates to subcellular energy and redox balance was investigated in leaf protoplasts of barley (Hordeum vulgare L.). This elegant study examined both spatial and temporal resolution of metabolism and indicated the importance of glycine oxidation in mitochondria in energisation of the cytosol and chloroplasts and in maintaining redox balance in the light and during the first minute following illumination.
-
Igamberdiev A.U., Romanowska E., Gardestrom P. Photorespiratory flux and mitochondrial contribution to energy and redox balance of barley leaf protoplasts in the light and during light-dark transitions. J. Plant Physiol. 158:2001;1325-1332 In this paper the contribution of mitochondrial oxidation of photorespiratory and respiratory substrates to subcellular energy and redox balance was investigated in leaf protoplasts of barley (Hordeum vulgare L.). This elegant study examined both spatial and temporal resolution of metabolism and indicated the importance of glycine oxidation in mitochondria in energisation of the cytosol and chloroplasts and in maintaining redox balance in the light and during the first minute following illumination.
-
(2001)
J. Plant Physiol.
, vol.158
, pp. 1325-1332
-
-
Igamberdiev, A.U.1
Romanowska, E.2
Gardestrom, P.3
-
29
-
-
0001693543
-
Subcellular metabolite determination in spinach leaves through non-aqueous fractionation
-
Gerhardt R., Stitt M., Heldt H.W. Subcellular metabolite determination in spinach leaves through non-aqueous fractionation. Physiol. Chem. 364:1983;1130-1131.
-
(1983)
Physiol. Chem.
, vol.364
, pp. 1130-1131
-
-
Gerhardt, R.1
Stitt, M.2
Heldt, H.W.3
-
30
-
-
0034778216
-
Analysis of the compartmentation of glycolytic intermediates, nucleotides, sugars, organic acids, amino acids and sugar alcohols in potato tubers using a non-aqueous fractionation method
-
•]).
-
•]).
-
(2001)
Plant Physiol.
, vol.127
, pp. 685-700
-
-
Farre, E.M.1
Tiessen, A.2
Roessner, U.3
Geigenberger, P.4
Trethewey, R.N.5
Willmitzer, L.6
-
31
-
-
0001684722
-
The intercellular compartmentation of metabolites in leaves of Zea mays L
-
Leegood R.C. The intercellular compartmentation of metabolites in leaves of Zea mays L. Planta. 164:1985;163-171.
-
(1985)
Planta
, vol.164
, pp. 163-171
-
-
Leegood, R.C.1
-
32
-
-
0036845188
-
Construction of a specialized cDNA library from plant cells isolated by laser capture microdissection: Toward comprehensive analysis of the genes expressed in the rice phloem
-
Laser capture microdissection (LCM) is a powerful system that allows the isolation of selectively targeted cells from a tissue section for the analysis of gene-expression profiles of individual cells. This paper is the first to report the application of LCM to the gene expression analysis of plant cells. The possibility of using similar methods for analysing plant proteins or metabolites has not yet been explored.
-
Asano T., Masumura T., Kusano H., Kikuchi S., Kurita A., Shimada H., Kadowaki K. Construction of a specialized cDNA library from plant cells isolated by laser capture microdissection: toward comprehensive analysis of the genes expressed in the rice phloem. Plant J. 32:2002;41-49 Laser capture microdissection (LCM) is a powerful system that allows the isolation of selectively targeted cells from a tissue section for the analysis of gene-expression profiles of individual cells. This paper is the first to report the application of LCM to the gene expression analysis of plant cells. The possibility of using similar methods for analysing plant proteins or metabolites has not yet been explored.
-
(2002)
Plant J.
, vol.32
, pp. 41-49
-
-
Asano, T.1
Masumura, T.2
Kusano, H.3
Kikuchi, S.4
Kurita, A.5
Shimada, H.6
Kadowaki, K.7
-
33
-
-
85047686731
-
Microdissection of histologic sections: Past, present, and future
-
Eltoum I.A., Siegal G.P., Frost A.R. Microdissection of histologic sections: past, present, and future. Adv. Anat. Pathol. 9:2002;316-322.
-
(2002)
Adv. Anat. Pathol.
, vol.9
, pp. 316-322
-
-
Eltoum, I.A.1
Siegal, G.P.2
Frost, A.R.3
-
34
-
-
0031702848
-
High-resolution histographical mapping of glucose concentrations in developing cotyledons of Vicia faba in relation to mitotic activity and storage processes: Glucose as a possible developmental trigger
-
Borisjuk L., Walenta S., Weber H., Mueller-Klieser W., Wobus U. High-resolution histographical mapping of glucose concentrations in developing cotyledons of Vicia faba in relation to mitotic activity and storage processes: glucose as a possible developmental trigger. Plant J. 15:1998;583-591.
-
(1998)
Plant J.
, vol.15
, pp. 583-591
-
-
Borisjuk, L.1
Walenta, S.2
Weber, H.3
Mueller-Klieser, W.4
Wobus, U.5
-
35
-
-
0036008982
-
Spatial analysis of plant metabolism: Sucrose imaging within Vicia faba cotyledons reveals specific developmental patterns
-
This elegant study continues (see also [34]) the development and application of techniques to achieve the spatial resolution of key metabolites by a refinement of histochemical staining on plant sections. Metabolites are detected by coupled enzymic assays leading to the formation of NAD(P)H, which is visualised via bioluminescence. In this and the previous paper, glucose and sucrose gradients were measured across sections of developing Vicia seeds. It is shown that areas of the seed characterised by cell division contain high glucose, whereas areas characterised by storage contain high sucrose.
-
Borisjuk L., Walenta S., Rolletschek H., Mueller-Klieser W., Wobus U., Weber H. Spatial analysis of plant metabolism: sucrose imaging within Vicia faba cotyledons reveals specific developmental patterns. Plant J. 29:2002;521-530 This elegant study continues (see also [34]) the development and application of techniques to achieve the spatial resolution of key metabolites by a refinement of histochemical staining on plant sections. Metabolites are detected by coupled enzymic assays leading to the formation of NAD(P)H, which is visualised via bioluminescence. In this and the previous paper, glucose and sucrose gradients were measured across sections of developing Vicia seeds. It is shown that areas of the seed characterised by cell division contain high glucose, whereas areas characterised by storage contain high sucrose.
-
(2002)
Plant J.
, vol.29
, pp. 521-530
-
-
Borisjuk, L.1
Walenta, S.2
Rolletschek, H.3
Mueller-Klieser, W.4
Wobus, U.5
Weber, H.6
-
36
-
-
0034978567
-
Using histochemistry to study plant metabolism: The examples of its use in the immunolocalisation of amino acids in plant tissues, and of phosphenolpyruvate carboxykinase and its possible role in pH regulation
-
Walker R.P., Chen Z.H., Johnson K.E., Famiani F., Tecis L., Leegood R.C. Using histochemistry to study plant metabolism: the examples of its use in the immunolocalisation of amino acids in plant tissues, and of phosphenolpyruvate carboxykinase and its possible role in pH regulation. J. Exp. Bot. 52:2000;565-576.
-
(2000)
J. Exp. Bot.
, vol.52
, pp. 565-576
-
-
Walker, R.P.1
Chen, Z.H.2
Johnson, K.E.3
Famiani, F.4
Tecis, L.5
Leegood, R.C.6
-
37
-
-
0037162304
-
Visualisation of maltose uptake in living yeast cells by fluorescent nanosensors
-
This paper highlights the sensitivity of a breakthrough technology in which mutant periplasmic binding proteins coupled to two green fluorescent proteins can be monitored by FRET to act as nanosensors for their binding partners. As a proof-of-concept the authors created mutants with varied binding affinities for maltose and showed dynamic in vivo imaging of cytosolic maltose concentrations in yeast cells. With further development this study opens up the possibility of monitoring dynamic changes in the concentrations of a wide range of solutes at high resolution and should allow rapid progress in the understanding of solute transport between cells.
-
Fehr M., Frommer W.B., Lalonde S. Visualisation of maltose uptake in living yeast cells by fluorescent nanosensors. Proc. Natl. Acad. Sci. U.S.A. 99:2002;9846-9851 This paper highlights the sensitivity of a breakthrough technology in which mutant periplasmic binding proteins coupled to two green fluorescent proteins can be monitored by FRET to act as nanosensors for their binding partners. As a proof-of-concept the authors created mutants with varied binding affinities for maltose and showed dynamic in vivo imaging of cytosolic maltose concentrations in yeast cells. With further development this study opens up the possibility of monitoring dynamic changes in the concentrations of a wide range of solutes at high resolution and should allow rapid progress in the understanding of solute transport between cells.
-
(2002)
Proc. Natl. Acad. Sci. U.S.A.
, vol.99
, pp. 9846-9851
-
-
Fehr, M.1
Frommer, W.B.2
Lalonde, S.3
-
38
-
-
0037162527
-
Imaging of metabolites using a fusion protein between a periplasmic binding protein and GFP derivatives: From a chimera to a view of reality
-
Stitt M. Imaging of metabolites using a fusion protein between a periplasmic binding protein and GFP derivatives: from a chimera to a view of reality. Proc. Natl. Acad. Sci. U.S.A. 99:2000;9614-9616.
-
(2000)
Proc. Natl. Acad. Sci. U.S.A.
, vol.99
, pp. 9614-9616
-
-
Stitt, M.1
-
39
-
-
0036010142
-
Steps towards an integrated view of nitrogen metabolism
-
Stitt M., Muller C., Matt P., Gibon Y., Carillo P., Morcuende R., Scheible W.R. Steps towards an integrated view of nitrogen metabolism. J. Exp. Bot. 53:2002;959-970.
-
(2002)
J. Exp. Bot.
, vol.53
, pp. 959-970
-
-
Stitt, M.1
Muller, C.2
Matt, P.3
Gibon, Y.4
Carillo, P.5
Morcuende, R.6
Scheible, W.R.7
-
40
-
-
0036010141
-
Coordination of leaf minor amino acid contents in crop species: Significance and interpretation
-
In this work, amino acid contents were measured in a large number of samples taken from wheat, potato and barley leaves under different photosynthetic conditions. The variability in summed minor amino acid contents was approximately 20-fold across these conditions and did not correlate with short-term changes in primary carbon and nitrogen metabolism. However, striking linear relationships between the contents of most minor amino acids were observed, demonstrating that the contents of many minor amino acids vary in concert and suggesting that amino acid contents are coordinated across biosynthetic families.
-
Noctor G., Novitskaya L., Lea P.J., Foyer C.H. Coordination of leaf minor amino acid contents in crop species: significance and interpretation. J. Exp. Bot. 53:2002;939-945 In this work, amino acid contents were measured in a large number of samples taken from wheat, potato and barley leaves under different photosynthetic conditions. The variability in summed minor amino acid contents was approximately 20-fold across these conditions and did not correlate with short-term changes in primary carbon and nitrogen metabolism. However, striking linear relationships between the contents of most minor amino acids were observed, demonstrating that the contents of many minor amino acids vary in concert and suggesting that amino acid contents are coordinated across biosynthetic families.
-
(2002)
J. Exp. Bot.
, vol.53
, pp. 939-945
-
-
Noctor, G.1
Novitskaya, L.2
Lea, P.J.3
Foyer, C.H.4
-
41
-
-
0035201029
-
High-resolution metabolic phenotyping of genetically and environmentally diverse potato tuber systems. Identification of phenocopies
-
Roessner U., Willmitzer L., Fernie A.R. High-resolution metabolic phenotyping of genetically and environmentally diverse potato tuber systems. Identification of phenocopies. Plant Physiol. 127:2001;749-764.
-
(2001)
Plant Physiol.
, vol.127
, pp. 749-764
-
-
Roessner, U.1
Willmitzer, L.2
Fernie, A.R.3
-
42
-
-
0347664073
-
Photorespiration-dependent increases in phosphoenolpyruvate carboxylase, isocitrate dehydrogenase and glutamate dehydrogenase in tobacco plants deficient in ferredoxin-dependent glutamine-α-ketoglutarate amino transferase
-
••]).
-
••]).
-
(2002)
Planta
, vol.214
, pp. 877-886
-
-
Ferrario-Mery, S.1
Hodges, M.2
Hirel, B.3
Foyer, C.H.4
-
43
-
-
0033831570
-
Genomic analysis of a nutrient response in Arabidopsis reveals diverse expression patterns and novel metabolic and potential regulatory genes induced by nitrate
-
Wang R.C., Guegler K., LaBrie S.T., Crawford N.M. Genomic analysis of a nutrient response in Arabidopsis reveals diverse expression patterns and novel metabolic and potential regulatory genes induced by nitrate. Plant Cell. 12:2000;1491-1509.
-
(2000)
Plant Cell
, vol.12
, pp. 1491-1509
-
-
Wang, R.C.1
Guegler, K.2
LaBrie, S.T.3
Crawford, N.M.4
-
44
-
-
85012703084
-
A theoretical background to the use of measured intermediates in the study of the control of intermediary metabolism
-
Rolleston F.S. A theoretical background to the use of measured intermediates in the study of the control of intermediary metabolism. Curr. Top Cell Regul. 5:1972;47-75.
-
(1972)
Curr. Top Cell Regul.
, vol.5
, pp. 47-75
-
-
Rolleston, F.S.1
-
45
-
-
0036787226
-
Altered metabolic fluxes result from shifts in metabolite levels in sucrose phosphorylase-expressing potato tubers
-
Fernie A.R., Tiessen A., Stitt M., Willmitzer L., Geigenberger P. Altered metabolic fluxes result from shifts in metabolite levels in sucrose phosphorylase-expressing potato tubers. Plant Cell Envion. 25:2002;1219-1232.
-
(2002)
Plant Cell Envion.
, vol.25
, pp. 1219-1232
-
-
Fernie, A.R.1
Tiessen, A.2
Stitt, M.3
Willmitzer, L.4
Geigenberger, P.5
-
46
-
-
0031401175
-
Sugar-starvation-induced changes of carbon metabolism in excised maize root tips
-
Dieuaide-Noubhani M., Canioni P., Raymond P. Sugar-starvation-induced changes of carbon metabolism in excised maize root tips. Plant Phys. 115:1997;1505-1513.
-
(1997)
Plant Phys.
, vol.115
, pp. 1505-1513
-
-
Dieuaide-Noubhani, M.1
Canioni, P.2
Raymond, P.3
-
47
-
-
0032566703
-
Unidirectional steady-state rates of central metabolism enzymes measured simultaneously in a living plant tissue
-
Roscher A., Emsley L., Raymond P., Roby C. Unidirectional steady-state rates of central metabolism enzymes measured simultaneously in a living plant tissue. J. Biol. Chem. 273:1998;25053-25061.
-
(1998)
J. Biol. Chem.
, vol.273
, pp. 25053-25061
-
-
Roscher, A.1
Emsley, L.2
Raymond, P.3
Roby, C.4
-
48
-
-
0036189810
-
Quantitative analysis of intracellular metabolic fluxes using GC/MS and two-dimensional NMR spectroscopy
-
Yang C., Hua Q., Shimizu K. Quantitative analysis of intracellular metabolic fluxes using GC/MS and two-dimensional NMR spectroscopy. J. Biosci. Bioeng. 93:2002;78-87.
-
(2002)
J. Biosci. Bioeng.
, vol.93
, pp. 78-87
-
-
Yang, C.1
Hua, Q.2
Shimizu, K.3
-
49
-
-
0035014718
-
Central carbon metabolism of Saccharomyces cerevisiae explored by biosynthetic fractional C-13 labelling of common amino acids
-
Maaheimo H., Fiaux J., Cakar Z.P., Bailey J.E., Sauer U., Szyperski T. Central carbon metabolism of Saccharomyces cerevisiae explored by biosynthetic fractional C-13 labelling of common amino acids. Eur. J. Biochem. 268:2001;2464-2479.
-
(2001)
Eur. J. Biochem.
, vol.268
, pp. 2464-2479
-
-
Maaheimo, H.1
Fiaux, J.2
Cakar, Z.P.3
Bailey, J.E.4
Sauer, U.5
Szyperski, T.6
-
50
-
-
0036743460
-
Starch synthesis in potato tubers is regulated by post-translational redox modification of ADP-glucose pyrophosphorylase: A novel regulatory mechanism linking starch synthesis to the sucrose supply
-
The authors demonstrate a novel mechanism to regulate starch synthesis that involves post-translational redox modification of AGPase. Analysis of the catalytic subunit of AGPase, AGPB, on native gels revealed that AGPB is present as a mixture of monomers and dimers in growing tubers. AGPB becomes completely dimerised when tubers are detached. This dimerisation led to the inactivation of AGPase due to decreased substrate affinity and insensitivity to allosteric effectors. Reversal of dimerisation by the reductant dithiothreitol, suggests that dimerisation is redox-regulated. Furthermore, the activation state of AGPase correlated with sucrose content across a wide range of treatments.
-
Tiessen A., Hendriks J.H.M., Stitt M., Branscheid A., Gibon Y., Farre E.M., Geigenberger P. Starch synthesis in potato tubers is regulated by post-translational redox modification of ADP-glucose pyrophosphorylase: a novel regulatory mechanism linking starch synthesis to the sucrose supply. Plant Cell. 14:2002;2191-2213 The authors demonstrate a novel mechanism to regulate starch synthesis that involves post-translational redox modification of AGPase. Analysis of the catalytic subunit of AGPase, AGPB, on native gels revealed that AGPB is present as a mixture of monomers and dimers in growing tubers. AGPB becomes completely dimerised when tubers are detached. This dimerisation led to the inactivation of AGPase due to decreased substrate affinity and insensitivity to allosteric effectors. Reversal of dimerisation by the reductant dithiothreitol, suggests that dimerisation is redox-regulated. Furthermore, the activation state of AGPase correlated with sucrose content across a wide range of treatments.
-
(2002)
Plant Cell
, vol.14
, pp. 2191-2213
-
-
Tiessen, A.1
Hendriks, J.H.M.2
Stitt, M.3
Branscheid, A.4
Gibon, Y.5
Farre, E.M.6
Geigenberger, P.7
-
51
-
-
0034660447
-
14-3-3s regulate global cleavage of their diverse binding partners in sugar-starved Arabidopsis cells
-
This paper provides an elegant example of the power of generic methods to detect a particular class of post-translational protein modification. 14-3-3 proteins are involved in binding and regulating the interactions between many proteins and their binding requires phosphorylation of a consensus sequence. After separating proteins on a gel, incubation of 14-3-3 proteins coupled to dioxygenin allowed visualisation of all operative 14-3-3 binding targets in a given tissue and under defined conditions. This approach was used to reveal many 14-3-3 proteins involved in central carbon and nitrogen metabolism and showed that carbon starvation leads to their degradation.
-
Cotelle V., Meek S.E.M., Provan F., Milne F.C., Morrice N., MacKintosh C. 14-3-3s regulate global cleavage of their diverse binding partners in sugar-starved Arabidopsis cells. EMBO J. 19:2000;2869-2876 This paper provides an elegant example of the power of generic methods to detect a particular class of post-translational protein modification. 14-3-3 proteins are involved in binding and regulating the interactions between many proteins and their binding requires phosphorylation of a consensus sequence. After separating proteins on a gel, incubation of 14-3-3 proteins coupled to dioxygenin allowed visualisation of all operative 14-3-3 binding targets in a given tissue and under defined conditions. This approach was used to reveal many 14-3-3 proteins involved in central carbon and nitrogen metabolism and showed that carbon starvation leads to their degradation.
-
(2000)
EMBO J.
, vol.19
, pp. 2869-2876
-
-
Cotelle, V.1
Meek, S.E.M.2
Provan, F.3
Milne, F.C.4
Morrice, N.5
MacKintosh, C.6
-
52
-
-
0028819049
-
Regulation of metabolism in transgenic plants
-
Stitt M., Sonnewald U. Regulation of metabolism in transgenic plants. Annu. Rev. Plant Phys. 46:1995;341-368.
-
(1995)
Annu. Rev. Plant Phys.
, vol.46
, pp. 341-368
-
-
Stitt, M.1
Sonnewald, U.2
-
53
-
-
0033151521
-
Nitrate regulation of metabolism and growth
-
Stitt M. Nitrate regulation of metabolism and growth. Curr. Opin. Plant Biol. 2:1999;178-186.
-
(1999)
Curr. Opin. Plant Biol.
, vol.2
, pp. 178-186
-
-
Stitt, M.1
-
54
-
-
0035059568
-
A small decrease of plastid transketolase activity in antisense tobacco transformants has dramatic effects on photosynthesis and phenylpropanoid metabolism
-
This paper reveals that flux into the shikimic acid pathway for the synthesis of aromatic amino acids, and hence into phenylpropamoid metabolism, is surprisingly sensitive to small decreases in the expression of plastid transketolase. These results imply that this important segment of nitrogen and secondary metabolism is co-limited by the supply of the immediate substrate in primary metabolism.
-
Henkes S., Sonnewald U., Badur R., Flachmann R., Stitt M. A small decrease of plastid transketolase activity in antisense tobacco transformants has dramatic effects on photosynthesis and phenylpropanoid metabolism. Plant Cell. 13:2001;535-551 This paper reveals that flux into the shikimic acid pathway for the synthesis of aromatic amino acids, and hence into phenylpropamoid metabolism, is surprisingly sensitive to small decreases in the expression of plastid transketolase. These results imply that this important segment of nitrogen and secondary metabolism is co-limited by the supply of the immediate substrate in primary metabolism.
-
(2001)
Plant Cell
, vol.13
, pp. 535-551
-
-
Henkes, S.1
Sonnewald, U.2
Badur, R.3
Flachmann, R.4
Stitt, M.5
-
55
-
-
0036954664
-
Carbon assimilation and metabolism in potato leaves deficient in plastidial phosphoglucomutase
-
The analysis of a wide range of metabolites, in addition to the major photosynthetic intermediates and products, of potato plants deficient in plastidial phosphoglucomutase revealed a diversion of carbon from the starch to the amino acid biosynthetic pathways of the plastid. This study illustrates the power of the combination of organelle-targeted transgenic research and profiling technologies at dissecting pathway interrelationships at the subcellular level.
-
Lytovchenko A., Bieberich K., Willmitzer L., Fernie A.R. Carbon assimilation and metabolism in potato leaves deficient in plastidial phosphoglucomutase. Planta. 215:2002;802-811 The analysis of a wide range of metabolites, in addition to the major photosynthetic intermediates and products, of potato plants deficient in plastidial phosphoglucomutase revealed a diversion of carbon from the starch to the amino acid biosynthetic pathways of the plastid. This study illustrates the power of the combination of organelle-targeted transgenic research and profiling technologies at dissecting pathway interrelationships at the subcellular level.
-
(2002)
Planta
, vol.215
, pp. 802-811
-
-
Lytovchenko, A.1
Bieberich, K.2
Willmitzer, L.3
Fernie, A.R.4
-
56
-
-
0035160371
-
A functional genomics strategy that uses metabolome data to reveal the phenotype of silent mutations
-
Raamsdonk L.M., Teusink B., Broadhurst D., Zhang N.S., Hayes A., Walsh M.C., Berden J.A., Brindle K.M., Kell D.B., Rowland J.J.et al. A functional genomics strategy that uses metabolome data to reveal the phenotype of silent mutations. Nat. Biotechnol. 19:2000;45-50.
-
(2000)
Nat. Biotechnol.
, vol.19
, pp. 45-50
-
-
Raamsdonk, L.M.1
Teusink, B.2
Broadhurst, D.3
Zhang, N.S.4
Hayes, A.5
Walsh, M.C.6
Berden, J.A.7
Brindle, K.M.8
Kell, D.B.9
Rowland, J.J.10
-
57
-
-
0036671677
-
Transgenic 14-3-3 isoforms in plants: The metabolic profiling of repressed 14-3-3 protein synthesis in transgenic potato plants
-
Szopa J. Transgenic 14-3-3 isoforms in plants: the metabolic profiling of repressed 14-3-3 protein synthesis in transgenic potato plants. Biochem. Soc. Trans. 30:2002;405-410.
-
(2002)
Biochem. Soc. Trans.
, vol.30
, pp. 405-410
-
-
Szopa, J.1
-
58
-
-
0035103179
-
Regulation of plant NR activity by reversible phosphorylation, 14-3-3 proteins and proteolysis
-
2+, protein kinases, protein kinase kinases, protein phosphatases, 14-3-3 proteins and proteases. Extracellular sugars exert a major impact on the 14-3-3-binding status and stability of many target proteins, including NR in plants. The authors suggest that this regulation is an example of how selective or global switches in cellular behaviour are triggered by regulatory networks in response to environmental cues.
-
2+, protein kinases, protein kinase kinases, protein phosphatases, 14-3-3 proteins and proteases. Extracellular sugars exert a major impact on the 14-3-3-binding status and stability of many target proteins, including NR in plants. The authors suggest that this regulation is an example of how selective or global switches in cellular behaviour are triggered by regulatory networks in response to environmental cues.
-
(2001)
Cell Mol. Life Sci.
, vol.58
, pp. 205-214
-
-
MacKintosh, C.1
Meek, S.E.M.2
-
59
-
-
0032754788
-
The nitrate and ammonium nitrate supply have a major influence on the response of photosynthesis, carbon metabolism, nitrogen metabolism and growth to elevated carbon dioxide in tobacco
-
Geiger M., Haake V., Ludewig F., Sonnewald U., Stitt M. The nitrate and ammonium nitrate supply have a major influence on the response of photosynthesis, carbon metabolism, nitrogen metabolism and growth to elevated carbon dioxide in tobacco. Plant Cell Environ. 22:1999;1177-1199.
-
(1999)
Plant Cell Environ.
, vol.22
, pp. 1177-1199
-
-
Geiger, M.1
Haake, V.2
Ludewig, F.3
Sonnewald, U.4
Stitt, M.5
-
60
-
-
0036042222
-
Metabolomics and machine learning: Explanatory analysis of complex metabolome data using genetic programming to produce simple, robust rules
-
Kell D.B. Metabolomics and machine learning: explanatory analysis of complex metabolome data using genetic programming to produce simple, robust rules. Mol. Biol. Rep. 29:2002;237-241.
-
(2002)
Mol. Biol. Rep.
, vol.29
, pp. 237-241
-
-
Kell, D.B.1
-
61
-
-
0000354617
-
The Arabidopsis genome initiative: Analysis of the genome sequence of the flowering plant Arabidopsis thaliana
-
The Arabidopsis genome initiative: Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 2000, 408:796-815.
-
(2000)
Nature
, vol.408
, pp. 796-815
-
-
-
62
-
-
0033764285
-
The challenges of in silico biology
-
Palsson B. The challenges of in silico biology. Nat. Biotechnol. 18:2000;1147-1150.
-
(2000)
Nat. Biotechnol.
, vol.18
, pp. 1147-1150
-
-
Palsson, B.1
-
63
-
-
85047681400
-
Improving plant breeding with exotic genetic libraries
-
This review highlights recent advances in plant breeding and the diversity of breeding populations available for study, discussing the fact that such plants represent excellent material for broad based metabolic studies.
-
Zamir D. Improving plant breeding with exotic genetic libraries. Nat. Rev. Genet. 2:2001;983-989 This review highlights recent advances in plant breeding and the diversity of breeding populations available for study, discussing the fact that such plants represent excellent material for broad based metabolic studies.
-
(2001)
Nat. Rev. Genet.
, vol.2
, pp. 983-989
-
-
Zamir, D.1
-
64
-
-
0035039867
-
Genetic analysis of amino acid accumulation in opaque-2 maize endosperm
-
Wang X.L., Larkins B.A. Genetic analysis of amino acid accumulation in opaque-2 maize endosperm. Plant Physiol. 125:2001;1766-1777.
-
(2001)
Plant Physiol.
, vol.125
, pp. 1766-1777
-
-
Wang, X.L.1
Larkins, B.A.2
-
65
-
-
0036931344
-
Metabolic profiling and biochemical phenotyping of plant systems
-
Roessner U., Willmitzer L., Fernie A.R. Metabolic profiling and biochemical phenotyping of plant systems. Plant Cell Reports. 21:2002;189-196.
-
(2002)
Plant Cell Reports
, vol.21
, pp. 189-196
-
-
Roessner, U.1
Willmitzer, L.2
Fernie, A.R.3
|