Expression and biochemical characterization of the 1-HO-carotenoid methylase CrtF from Rhodobacter capsulatus

FEMS Microbiology Letters

Volumn 222, Issue 2, 2003, Pages 237-242

  Badenhop, Frank ^a   Steiger, Sabine ^a   Sandmann, Manuela ^a   Sandmann, Gerhard ^a  

^a GOETHE UNIVERSITY   (Germany)

Author keywords

Acyclic 1 HO carotenoid methylase; CrtF; Rhodobacter capsulatus; Spheroidene biosynthesis; Spirilloxanthin biosynthesis

Indexed keywords

BACTERIAL ENZYME; CAROTENOID METHYLASE; HYDROXYL GROUP; S ADENOSYLMETHIONINE; TETRODOTOXIN; UNCLASSIFIED DRUG; XANTHINE;

AFFINITY CHROMATOGRAPHY; ARTICLE; BIOSYNTHESIS; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME MECHANISM; ENZYME PURIFICATION; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENETIC COMPLEMENTATION; METHYLATION; NONHUMAN; PHOTOTROPHIC BACTERIUM; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; RHODOBACTER CAPSULATUS; SPECIES COMPARISON;

ESCHERICHIA COLI; PROTEOBACTERIA; RHODOBACTER CAPSULATUS;

EID: 0038735537     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(03)00302-1     Document Type: Article

References (21)

1. Schmidt, K. (1978) Biosynthesis of carotenoids. In: The Photosynthetic Bacteria (Clayton, R.K. and Sistrom, W.R., Eds.) pp. 729-70, Plenum Press, New York.
2. Takaichi, S. (1999) Carotenoids and carotenogenesis in anoxygenic bacteria. In: The Photochemistry of Carotenoids (Frank, H.A., Young, A.J., and Cogdell, R.E., Eds.) pp. 39-69. Kluwer Academic Publisher, Dordrecht.
3. Sandmann G. Carotenoid biosynthesis in microorganisms and plants. Eur. J. Biochem. 223:1994;7-24.
4. Albrecht M., Ruther A., Sandmann G. Purification and biochemical characterization of a hydroxyneurosporene desaturase involved in the biosynthetic pathway of the carotenoid spheroidene in Rhodobacter sphaeroides. J. Bacteriol. 179:1997;7462-7467.
5. Steiger S., Astier C., Sandmann G. Substrate specificity of the expressed 3,4-carotenoid desaturase from Rubrivivax gelatinosus reveals the detailed reaction sequence to spheroidene and spirilloxanthin. Biochem. J. 349:2000;635-640.
6. Scolnik P.A., Walker M.A., Marrs B.L. Biosynthesis of carotenoids derived from neurosporene in Rhodopseudomonas capsulata. J. Biol. Chem. 255:1980;2427-2432.
7. Singh R.K., Britton G., Goodwin T.W. Carotenoid biosynthesis in Rhodopseudomonas sphaeroides. S-Adenosylmethionine as the methylation agent in the biosynthesis of spheroidene and spheroidenone. Biochem. J. 136:1973;413-419.
8. Armstrong G.A., Alberti M., Leach F., Hearst J.E. Nucleotide sequence, organisation, and nature of the protein products of the carotenoid biosynthesis gene cluster of Rhodobacter capsulatus. Mol. Gen. Genet. 216:1989;254-268.
9. Ditta G., Schmidhauser T., Yakobson E., Lu P., Liang X.-W., Finlay D.R., Guiney D., Helinski D.R. Plasmids related to the broad range vector, pRK290, useful for gene cloning and for monitoring gene expression. Plasmid. 13:1985;149-153.
10. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 33:1985;103-119.
11. Boyle S.M., Markham G.D., Hafner E.W., Wright J.M., Tabor H., White Tabor C. Expression of the cloned genes encoding the putrescine biosynthetic enzymes and methionine adenosyltransferase of Escherichia coli (speA, speB, speC and metK). Gene. 30:1984;129-136.
12. Verdoes J., Krubasik P., Sandmann G., van Ooyen M. Isolation and functional characterisation of a novel type of carotenoid biosynthetic gene from Xanthophyllomyces dendrorhous. Molec. Gen. Genet. 262:1999;453-461.
13. Sandmann G. Combinatorial biosynthesis of carotenoids in a heterologous host: A powerful approach for the biosynthesis of novel structures. ChemBioChem. 3:2002;629-635.
14. Britton G. General carotenoid methods. Methods Enzymol. 111:1985;113-149.
15. Davis, B.H. (1976) Carotenoids. In: Biochemistry of Plant Pigments. Vol. 2. (Goodwin, T.W., Ed.), pp. 38-365. Academic Press, London.
16. Laemmli U.K. Cleavage of structural protein during assembly of the head of bacteriophage T4. Nature. 227:1979;680-688.
17. Park J., Tai J., Roessner C.A., Scott A.I. Enzymatic synthesis of S-adenosyl-L-methionine on a preparative scale. Biorg. Med. Chem. 4:1996;2179-2185.
18. Komori M., Ghosh R., Takaichi S., Hu Y., Mizoguchi T., Koyama Y., Kuki M. A null lesion in the rhodopin 3,4-desaturase of Rhodospirillum rubrum unmasks a cryptic branch of the carotenoid biosynthetic pathway. Biochemistry. 37:1998;8987-8994.
19. Steiger S., Takaichi S., Sandmann G. Heterologous production of two novel acyclic carotenoids, 1,1′-dihydroxy-3,4-didehydrolycopene and 1-hydroxy-3,4,3′,4′- tetradehydrolycopene by combination of the crtC and crtD genes from Rhodobacter and Rubrivivax. J. Biotechnol. 97:2002;51-58.
20. Blackwell J.R., Horgan R. A novel strategy for production of a highly expressed recombinant protein in an active form. FEBS Lett. 295:1991;10-12.
21. Lucht J.M., Bremer E. Adaptation of Escherichia coli to high osmolarity environments: Osmoregulation of the high-affinity glycine betaine transport system ProU. FEMS Microbiol. Rev. 14:1994;3-20.