Pregnancy rates in mares after a single fixed time hysteroscopic insemination of low numbers of frozen-thawed spermatozoa onto the uterotubal junction

Equine Veterinary Journal

Volumn 35, Issue 2, 2003, Pages 197-201

  Morris, L H A ^a,b   Tiplady, C ^a   Allen, W R ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b Equine Fertility Services   (New Zealand)

Author keywords

Frozen thawed spermatozoa; Horse; Hysteroscopic insemination; Low dose insemination; Uterotubal junction

Indexed keywords

CHORIONIC GONADOTROPIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; ARTIFICIAL INSEMINATION; CONTROLLED STUDY; FEMALE; FERTILITY; FREEZING; HORSE; HYSTEROSCOPY; INTERMETHOD COMPARISON; MALE; NONHUMAN; OVARY FOLLICLE; OVULATION INDUCTION; PREGNANCY RATE; SPERM PRESERVATION; SPERMATOZOON; SPERMATOZOON COUNT; THAWING; UTERUS; ANIMAL; ANIMAL DISEASE; COMPARATIVE STUDY; GENETIC ENGINEERING; METHODOLOGY; PHYSIOLOGY; PREGNANCY;

ANIMALIA; EQUUS CABALLUS;

ANIMALS; CHORIONIC GONADOTROPIN; FEMALE; HORSES; HYSTEROSCOPY; INSEMINATION, ARTIFICIAL; MALE; OVULATION INDUCTION; PREGNANCY; PREGNANCY RATE; SEMEN PRESERVATION; SEX PRESELECTION; SPERM COUNT; SPERMATOZOA;

EID: 0038728284     PISSN: 04251644     EISSN: None     Source Type: Journal    
DOI: 10.2746/042516403776114162     Document Type: Article

References (33)

1. Allen, W.R. and Sanderson, M.W. (1987) The value of a rapid progesterone assay (AELIA) in equine stud veterinary medicine and management. In: Proceedings of the 9th Bain Fallon Memorial Lectures, Australian Equine Veterinary Association. pp 75-82.
2. Allen, W.R., Urwin, V., Simpson, D.J., Greenwood, R.E.S., Crowhurst, R.C., Ellis, D.R., Ricketts, S.W., Hunt, M.D.N. and Wingfield-Digby, N.J. (1980) Preliminary studies on the use of oral progestagen to induce oestrus and ovulation in seasonally anoestrous thoroughbred mares. Equine vet. J. 12, 141-145.
3. Barbacini, S., Zavaglia, G., Gulden, P., Marchi, V. and Necchi, D. (2000) Retrospective study on the efficiency of hCG in an equine artificial insemination programme using frozen semen. Equine vet. Educ. 12, 312-317.
4. Buchanan, B.R., Seidel, G.E. Jr, McCue, P.M., Schenk, J.L., Herickhoff, L.A. and Squires, E.L. (2000) Insemination of mares with either unsexed or sexed spermatozoa. Theriogenology 53, 1333-1344.
5. Byrd, W., Bradshaw, K., Carr, B., Edman, C., Odom, J. and Ackerman, G. (1990) A prospective randomized study of pregnancy rates following intrauterine and intracervical insemination using frozen donor sperm. Fertil. Steril. 53, 521-527.
6. Dobrinski, I., Thomas, P.G. and Ball, B. (1995) Cryopreservation reduces the ability of equine spermatozoa to attach to oviductal epithelial cells and zonae pellucidae in vitro. J. Androl. 16, 536-542.
7. Drobnis, E.Z., Clisham P.R., Brazil, C.K., Wisner, L.W., Zhong, C.Q. and Overstreet, J.W. (1993) Detection of altered acrosomal physiology of cryopreserved human spermatozoa after sperm residence in the female reproductive tract. J. Reprod. Fert. 99, 159-165.
8. Heitland, A.V., Jasko, D.J., Squires, E.L., Graham, J.K., Pickett, B.W. and Hamilton, C. (1996) Factors affecting motion characteristics of frozen-thawed stallion spermatozoa. Equine vet. J. 28, 47-53.
9. Hill, J.R., Thompson, J.A. and Perkins, N.R. (1998) Factors affecting pregnancy rates following laparoscopic insemination of 28,477 Merino ewes under commercial conditions: a survey. Theriogenology 49, 697-709.
10. Holt, W.V. and North, R.D. (1994) Effects of temperature and restoration of osmotic equilibrium during thawing on the induction of plasma membrane damage in cryopreserved ram spermatozoa. Biol. Reprod. 51, 414-424.
11. Hyland, J.H. and Bristol, F. (1979) Synchronisation of oestrus and timed insemination of mares. J. Reprod. Fert., Suppl. 27, 251-255.
12. Katila, T., Sankari, S. and Makela, O. (2000) Transport of spermatozoa in the genital tracts of mares. J. Reprod. Fert., Suppl. 56, 571-578.
13. Kenney, R.M., Bergman, R.V., Cooper, W.L. and Morse, G.W. (1975) Minimal contamination techniques for breeding mares: technique and preliminary findings. Proc. Am. Ass. equine Practnrs. 21, 327-335.
14. Kloppe, L.H., Varner, D.D., Elmore, R.G., Bretzlaff, K.N. and Shull, J.W. (1988) Effect of insemination timing on the fertilizing capacity of frozen-thawed equine spermatozoa. Theriogenology 29, 429-439.
15. Lefebvre, R. and Samper, J.C. (1993) Interactions between stallion spermatozoa and oviductal epithelial cells in vitro. Equine vet. J., Suppl. 15, 39-41.
16. Lightfoot, R.J. and Salamon, S. (1970) Fertility of ram spermatozoa frozen by the pellet method. I. Transport and viability of spermatozoa within the genital tract of the ewe. J. Reprod. Fert. 22, 385-398.
17. Lindsey, A.C., Morris, L.H.A., Allen, W.R. Schenk, J.L., Squires, E.L. and Bruemmer, J.E. (2002) Hysteroscopic insemination of mares with low numbers of nonsorted or flow sorted spermatozoa. Equine vet. J. 34, 128-132.
18. Loomis, P.R., Amann, R.P., Squires, E.L. and Pickett, B.W. (1983) Fertility of unfrozen and frozen stallion spermatozoa extended in EDTA-lactose-egg yolk and packaged in straws. J. anim. Sci. 56, 687-693.
19. Magistrini, M., Chanteloube, Ph. and Palmer, E. (1987) Influence of season and frequency of ejaculation on production of stallion semen for freezing. J. Reprod. Fert., Suppl. 35, 127-133.
20. Morris, L.H.A., Hunter, R.H.F. and Allen, W.R. (2000) Hysteroscopic insemination of small numbers of spermatozoa at the uterotubal junction of preovulatory mares. J. Reprod. Fert. 118, 95-100.
21. Morris, L.H.A., Hunter, R.H.F., Tiplady, C.A. and Allen, W.R. (2003) Low numbers of spermatozoa in the equine oviduct are compatible with fertilization. Reproduction. (in press).
22. Müller, Z. (1987) Practicalities of insemination of mares with deep frozen semen. J. Reprod. Fert., Suppl. 35, 121-125.
23. Pace, M.M. and Sullivan, J.J. (1975) Effect of timing of insemination, numbers of spermatozoa and extender components on the pregnancy rate in mares inseminated with frozen stallion semen. J. Reprod. Fert., Suppl. 23, 115-121.
24. Pantke, P., Hyland, J.H., Galloway, D.B., Lui, D.Y. and Baker, H.W.G. (1995) Development of a zona pellucida-sperm binding assay for the assessment of stallion fertility. In: Biology of Reproduction Monograph Series 1: Equine Reproduction VI. pp 681-687.
25. Parks, J.E. and Graham, J.K. (1992) Effects of cryopreservation procedures on sperm membranes. Theriogenology 38, 209-222.
26. Pickett, B.W., Voss, J.L., Squires, E.L., Vanderwall, D.K., McCue, P.M. and Breummer, J.E. (2000) Collection, Preparation and Insemination of Stallion semen. Bulletin No. 10. Animal Reproduction and Biotechnology Laboratory, Colorado State University, Colorado.
27. Samper, J.C. (1991) Relationship between the fertility of fresh and frozen stallion semen and semen quality. J. Reprod. Fert., Suppl. 44, 107-114.
28. Samper, J.C. (1999) Increasing pregnancy rates with frozen semen: can it be done? In: Proceedings of the Annual Meeting of the Society of Theriogenology. Nashville, Tennesse. pp 109-115.
29. Samper, J.C. and Morris, C.A. (1998) Current methods for stallion semen cropreservation. Theriogenology 49, 895-903.
30. Scott, M.A., Liu, I.K.M., Overstreet, J.W. and Enders, A.C. (2000) The structural morphology and epithelial association of spermatozoa at the uterotubal junction: a descriptive study of equine spermatozoa in situ using scanning electron microscopy. J. Reprod. Fert., Suppl. 56, 415-421.
31. Vidament, M., Dupere, A.M., Julienne, P., Evain, A., Noue, P. and Palmer, E. (1997) Equine frozen semen: freezability and fertility field results. Theriogenology 48, 907-917.
32. Voss, J.L., Wallace, R.A., Squires, E.L., Pickett, B.W. and Shideler, R.K. (1979) Effects of synchronisation and frequency of insemination on fertility. J. Reprod. Fert., Suppl. 27, 257-261.
33. Watson, P.F. (1995) Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post thawing function. Reprod. Fert. Dev. 7, 871-891.