Addition of a competitive primer can dramatically improve the specificity of PCR amplification of specific alleles

BioTechniques

Volumn 21, Issue 4, 1996, Pages 586-590

  Zhu, Kun Yan ^a   Clark, J Marshall ^a  

^a UNIVERSITY OF MASSACHUSETTS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PRIMER DNA;

ACCURACY; ALLELE; ARTICLE; POLYMERASE CHAIN REACTION;

EID: 0038712455     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96214bm04     Document Type: Article

References (9)

1. Kwok, S., D.E. Kellogg, N. McKinney, D. Spasic, L. Goda, C. Levenson and J.J. Sninsky. 1990. Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 model studies. Nucleic Acids Res. 18:999-1005.
2. Newton, C.R., A. Graham, L.E. Heptinstall, S.J. Powell, C. Summers, N. Kalsheker, J.C. Smith and A.F. Markham. 1989. Analysis of any point mutation in DNA: the amplification refractory mutation system (ARMS). Nucleic Acids Res. 17:2503-2516.
3. Okayama, H., D.T. Curiel, M.L. Brantly, M.D. Holmes and R.G. Crystal. 1989. Rapid nonradioactive detection of mutations in the human genome by allele-specific amplification. J. Lab. Clin. Med. 114:105-113.
4. Petruska, J., M.F. Goodman, M.S. Boosalis, L.C. Sowers, C. Cheong and I. Tinoco, Jr. 1988. Comparison between DNA melting thermodynamics and DNA polymerase fidelity. Proc. Natl. Acad. Sci. USA 85:6252-6256.
5. Sarkar, G., J. Cassady, C.D.K. Bottema and S.S. Sommer. 1990. Characterization of polymerase chain reaction amplification of specific alleles. Anal. Biochem. 186:64-68.
6. Sommer, S.S., A.R. Groszbach and C.D.K. Bottema. 1992. PCR amplification of specific alleles (PASA) is a general method for rapidly detecting known single-base changes. BioTechniques 12:82-87.
7. Steichen, J.C. and R.H. Ffrench-Constant. 1994. Amplification of specific cyclodiene insecticide alleles by the polymerase chain reaction. Pestic. Biochem. Physiol. 48:1-7.
8. Wu, D.Y., L. Ugozzoli, B.K. Pal and R.B. Wallace. 1989. Allele-specific enzymatic amplification of β-globulin genomic DNA for diagnosis of sickle cell anemia. Proc. Natl. Acad. Sci. USA 36:2757-2760.
9. Zhu, K.Y. and J.M. Clark. 1995. Cloning and sequencing of a cDNA encoding acetylcholinesterase in Colorado potato beetle, Leptinotarsa decemlineata (Say). Insect Biochem. Molec. Biol. 25:1129-1138.