Efficient mutagenesis method for producing the templates of single nucleotide polymorphisms

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 24, Issue 2, 2003, Pages 105-110

  Zhang, Jia ^a   Li, Kai ^a,b   Deng, Zemin ^c   Liao, Duanfang ^a   Fang, Weiyi ^a   Zhang, Xu ^d  

^a UNIVERSITY OF SOUTH CHINA   (China)

^b CHARLES R DREW UNIVERSITY OF MEDICINE AND SCIENCE   (United States)

^c JINAN UNIVERSITY   (China)

^d Genomapping Inc   (China)

Author keywords

Mutagenesis; Single nucleotide polymorphisms

Indexed keywords

DNA BASE; DNA POLYMERASE; NUCLEOTIDE; PRIMER DNA;

AMPLICON; ARTICLE; BASE MISPAIRING; FEASIBILITY STUDY; GENETIC SCREENING; MUTAGENESIS; NUCLEOTIDE SEQUENCE; RELIABILITY; RESTRICTION SITE; SEQUENCE ANALYSIS; SINGLE NUCLEOTIDE POLYMORPHISM;

BASE SEQUENCE; DEOXYRIBONUCLEASE ECORI; ESCHERICHIA COLI; GENETIC ENGINEERING; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; NUCLEIC ACID AMPLIFICATION TECHNIQUES; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE NUCLEOTIDE; SEQUENCE ANALYSIS, DNA; TEMPLATES, GENETIC;

EID: 0038696379     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/MB:24:2:105     Document Type: Article

References (15)

1. Cargill, M., Altshuler, D., Ireland, J., Sklar, P., Ardlie, K., Patil, N., et al. (1999) Characterization of single-nucleotide polymorphisms in coding regions of human genes. Nature Genet. 22, 231-238.
2. Halushka, M.K., Fan, J., Bentley, K., Hsie, L., Shen, N., Weder, A., et al. (1999) Patterns of single-nucleotide polymorphisms in candidate genes for blood-pressure homeostasis. Nature Genet. 22, 239-247.
3. Lindblad-Toh, K., Winchester, E., Daly, M.J. et al. (2000) Large-scale discovery and genotyping of single-nucleotidep olymorphisms in the mouse. Nature Genet. 24, 381-386.
4. Park, S.J., Taton, A., and Mirkin, C.A. (2002) Array-based electrical detection of DNA with nanoparticle probes. Science 295, 1503-1506.
5. Zhang, J., and Li, K. (2001) The 3′ terminal labeled primer extension: A new method of high throughput screening for SNP analysis. Curr. Drug Discovery 9, 21-24.
6. Pastinen, T., Raitio, M., Lindroos, K., Tainola, P., Peltonen, L., Syvanen, A.C. (2000) A system for specific, high-throughput genotyping by allele-specific primer extension on microarrays. Genome Res. 10, 1031-1042.
7. Hill, D.E., Oliphant, A.R., and Struhl, K. (1987) Mutagenesis with degenerate oligonucleotides: An efficient method for saturating a defined DNA region with base pair substitutions. Methods Enzymol. 155, 558-568.
8. Kunkel, T.A., Roberts, J.D., and Zakour, RA. (1987) Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 154, 367-382.
9. Smith, M. (1985) In vitro mutagenesis. Ann. Rev. Genet. 19, 423-467.
10. Wallace, R.B., Schold, M., Johnson, M.J., et al. (1981) Oligonucleotide directed mutagenesis of the human β-globulin gene: A general method for producing specific point mutations in cloned DNA. Nucl. Acids Res. 9, 3647-3657.
11. Wells, J.A., Vasser, M., and Poweres, D.B. (1985) Cassette mutagenesis: An efficient method for generation of multiple mutations at defined sites. Gene 34, 315-323.
12. Huang, M.M., Arnheim, N., and Goodman, M.F. (1992) Extension of base mispairs by TAQ DNA polymerase: implications for single nucleotide discrimination in PCR. Nucl. Acids Res. 20, 4567-4573.
13. Kwok, D.E., Kellogg, D.E., McKinney, N., Spasic, D., Goda, I., Levenson, C., et al. (1990) Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 model studies. Nucl. Acids Res. 18, 995-1005.
14. Ørum, H., and Wengel, J. (2001) Locked nucleic acids: a promising molecular family for gene-function analysis and antisense drug development. Curr. Opin. Mol. Ther. 3, 239-243.
15. Martin, G. and Zhang, P. (2000) DNA chips/ microarrays and chemistry. Pharmaceuticals 2000 web conference. www.cruachem.co.uk/pages/micro/htm.