Analysis of intracellular cytokines using flowcytometry

Methods in Cell Science

Volumn 24, Issue 1-3, 2002, Pages 37-40

  Arora, Sunil K ^a  

^a POSTGRADUATE INSTITUTE OF MEDICAL EDUCATION AND RESEARCH   (India)

Author keywords

Cytokines; Flowcytometry; Intra cellular

Indexed keywords

CYTOKINE;

CELL ASSAY; CELL CLONING; CELL CULTURE; CELL PERMEABILIZATION; CELL STIMULATION; CELL SURFACE; CELLULAR DISTRIBUTION; CYTOKINE PRODUCTION; ENZYME LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; HUMAN; IMMUNE RESPONSE; IN SITU HYBRIDIZATION; PHENOTYPE; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN SYNTHESIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SHORT SURVEY; STAINING; T LYMPHOCYTE;

ANTIGENS, CD; CYTOKINES; FLOW CYTOMETRY; HUMANS; T-LYMPHOCYTES;

EID: 0038692960     PISSN: 13815741     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1024177428018     Document Type: Short Survey

References (7)

1. Isaacs D, MacDonald NE, Bangham CRM, McMichael AJ, Higgins PG, Tyrell DAJ (1991). The specific cytotoxic T-cell response of adult volunteers to infection with respiratory syncitial virus. Immun Infect Dis 1: 5.
2. Assenmacher M, Scheffold A, Schnitz J, Checa JAS, Miltenyi S, Radbruch A. (1996). Specific expression of surface interferon gamma on T-cells from mouse and man. Eur J Immunol 26: 263.
3. Pala P, Hussell T, Openshaw PJM (2000). Flow cytometric measurement of intracellular cytokines. J Immunol Meth 107-124.
4. Openshaw P, Murphy EE, Hosken NA, Maino V, Davis K, Murphy K, O'Garra A (1995). Heterogeneity of intracellular cytokine synthesis at the single cell level in polarized Th1 and Th2 populations. J Exp Med 182: 1357.
5. Sander B, Andersson J, Andersson U (1991). Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure. Immunol Rev 119: 65.
6. Lalvani A, Brookes R, Hambleton S, Britton WJ, Hill AV, McMichael AJ (1997). Rapid effector function in CD8+ memory T-cells. J Exp Med 186: 859.
7. Mosmann TR, Cherwinski HM, Bond MW, Geidlin MA, Coffman RL (1986). Two types of murine helper T-cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol 136: 2348.