Partial purification and characterization of an ascorbate-reducible b-type cytochrome from the plasma membrane of Arabidopsis thaliana leaves

Protoplasma

Volumn 221, Issue 1-2, 2003, Pages 47-56

  Berczi A ^a   Caubergs, R J ^b   Asard, H ^c  

^a INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

^b UNIVERSITY OF ANTWERP   (Belgium)

^c UNIVERSITY OF NEBRASKA LINCOLN   (United States)

Author keywords

Arabidopsis thaliana; Ascorbate reducible b type cytochrome; Fast protein liquid chromatography; Plasma membrane; Protein purification

Indexed keywords

ARABIDOPSIS; ASCORBIC ACID; CELL FRACTIONATION; CELL MEMBRANE; CYTOCHROMES B; HEME; OXIDATION-REDUCTION; PLANT LEAVES; SOLUBILITY; TITRIMETRY;

ANIMALIA; ARABIDOPSIS; ARABIDOPSIS THALIANA; PHASEOLUS VULGARIS;

EID: 0038687192     PISSN: 0033183X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00709-002-0060-5     Document Type: Conference Paper

References (45)

1. Annaert W (1993) A biochemical and morphological study of the secretory pathways in the synapto-adrenal system: implications on exocytosis and membrane recycling. PhD thesis, Universiteit Antwerpen (UIA), Antwerp, Belgium
2. Apps DK, Pryde JG, Phillips JH (1980) Cytochrome b561 is identical with chromomembrin B, a major polypeptide of chromaffin granule membranes. Neuroscience 5: 2279-2287
3. _ Boisclair MD, Gavine FS, Pettigrew GW (1984) Unusual redox behaviour of cytochrome b-561 from bovine chromaffin granule membranes. Biochim Biophys Acta 764: 8-16
4. Asard H, Bérczi A (1998) Comparison of the redox activities in plasma membranes from roots and shoots of etiolated bean seedlings. Protoplasma 205: 37-42
5. _ Venken M, Caubergs RJ, Reijnders W, Oltmann FL, De Greef JA (1989) b-Type cytochromes in higher plant plasma membranes. Plant Physiol 90: 1077-1083
6. _ Horemans N, Caubergs RJ (1992) Transmembrane electron transport in ascorbate-loaded plasma membrane vesicles from higher plants involves a b-type cytochrome. FEBS Lett 306: 143-146
7. _ Preger V, Trost P (1998) Plasma membrane b-type cytochromes. In: Asard H, Bérczi A, Caubergs RJ (eds) Plasma membrane redox systems and their role in biological stress and disease. Kluwer, Dordrecht, pp 1-32
8. _ Terol-Alcayde J, Preger V, Del Favero J, Verelst W, Sparla F, Pérez-Alonso M, Trost P (2000) Arabidopsis thaliana sequence analysis confirms the presence of cyt b-561 in plants: evidence for a novel protein family. Plant Physiol Biochem 38: 905-912
9. 561: a protein in search of a function. Protoplasma 217: 77-93
10. Askerlund P, Larsson C, Widell S (1989) Cytochromes of plant plasma membranes: characterization by absorbance difference spectroscopy and redox titration. Physiol Plant 76: 123-134
11. 561 protein family. Protoplasma 221: 31-40
12. 2+-ATPase activity in wheat root plasma membrane vesicles: time-dependence and effect of sucrose and detergents. Physiol Plant 70: 583-588
13. g2+-ATPase in microsomal fractions from wheat and maize roots. Physiol Plant 77: 12-19
14. _ Van Gestelen P, Pupillo P (1998) NAD(P)H-utilizing flavo-enzymes in the plant plasma membrane. In: Asard H, Bérczi A, Caubergs RJ (eds) Plasma membrane redox systems and their role in biological stress and disease. Kluwer, Dordrecht, pp 33-67
15. _ Lüthje S, Asard H (2001) b-Type cytochromes in plasma membranes of Phaseolus vulgaris hypocotyls, Arabidopsis thaliana leaves, and Zea mays roots. Protoplasma 217: 50-55
16. 2+-ATPase on the Golgi of corn coleoptiles. Plant Physiol 76: 498-507
17. Crane FL, Goldenberg H, Morré DJ, Löw H (1979) Dehydrogenases of the plasma membrane. In: Roodyn DB (ed) Subcellular biochemistry, vol 6. Plenum, New York, pp 345-399
18. _ Sun IL, Clark MG, Grebing C, Löw H (1985) Transplasma-membrane redox systems in growth and development. Biochim Biophys Acta 811: 233-264
19. Devreese B, Vanrobaeys F, Van Beeumen J (2001) Automated nanoflow liquid chromatography/tandem mass spectrometric identification of proteins from Shewanella putrefaciens separated by two-dimensional polyacrylamide gel electrophoresis. Rapid Commun Mass Spectrom 15: 50-56
20. Döring O, Lüthje S (1996) Molecular components and biochemistry of electron transport in plant plasma membranes. Mol Membr Biol 13: 127-142
21. 561 from bovine adrenal chromaffin granules. J Biol Chem 257: 8561-8564
22. Flatmark T, Grønberg M (1981) Cytochrome b-561 of the bovine adrenal chromaffin granules: molecular weight and hydrodynamic properties in micellar solutions of Triton X-100. Biochem Biophys Res Commun 99: 292-301
23. 561 of the bovine adrenal chromaffin granules: a high potential b-type cytochrome. Biochim Biophys Acta 253: 487-491
24. Heerklotz H, Binder H, Lantzsch G, Klose G (1994) Membrane/water partition of oligo(ethylene oxide) dodecyl ethers and its relevance for solubilization. Biochim Biophys Acta 1196: 114-122
25. Hodges TK, Leonard RT (1974) Purification of a plasma membrane-bound adenosine triphosphatase from plant roots. Methods Enzymol 32: 392-406
26. 2+ and polyamines. Plant Sci 43: 103-107
27. 561 is fatty acylated and oriented in the chromaffin granule membrane with its carboxyl terminus cytoplasmically exposed. J Biol Chem 265: 16422-16427
28. Koynova R, Tenchov B (2001) Interactions of surfactants and fatty acids with lipids. Curr Opin Colloid Interface Sci 6: 277-286
29. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685
30. n membranes: a time-resolved fuorescence study. Biophys Chem 58: 289-302
31. Larsson C, Sommarin M, Widell S (1994) Isolation of highly purified plant plasma membranes and separation of inside-out and right-side-out vesicles. Methods Enzymol 228: 451-469
32. London E, Brown DA (2000) Insolubility of lipids in Triton X-100: physical origin and relationship to sphingolipid/cholesterol membrane domains (rafts). Biochim Biophys Acta 1508: 182-195
33. Lüthje S, Böttger M (1995) On the function of a K-type vitamin in plasma membranes of maize (Zea mays L.) roots. Mitt Inst Allg Bot Hamburg 25: 5-13
34. _ Döring O, Heuer S, Lüthen H, Böttger M (1997) Oxidoreductases in plant plasma membranes. Biochim Biophys Acta 133l: 81-102
35. Markwell MAK, Haas SB, Bieber LL, Tolbert NE (1978) A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem 87: 206-210
36. Perin MS, Fried VA, Slaughter CA, Südhof TC (1988) The structure of cytochrome b561, a secretory vesicle-specific electron transport protein. EMBO J 7: 2697-2703
37. Ramirez JM, Gallego GG, Serrano R (1984) Electron transfer constituents in plasma membrane fractions of Avena sativa and Saccharomyces cerevisiae. Plant Sci Lett 34: 103-110
38. Robinson NJ, Procter CM, Connolly EL, Guerinot ML (1999) A ferric-chelate reductase for iron uptake from soils. Nature 397: 694-697
39. 561 of bean hypocotyl plasma membrane. Protoplasma 205: 66-73
40. Srivastava M, Gibson KR, Pollard HB, Fleming PJ (1994) Human cytochrome b561: a revised hypothesis for conformation in membranes which reconciles sequence and functional information. Biochem J 303: 915-921
41. Trost P, Bérczi A, Sparla F, Sponze G, Marzadori B, Asard H, Pupillo P (2000) Purification of cytochrome b-561 from bean hypocotyls plasma membrane: evidence for the presence of two heme centers. Biochim Biophys Acta 1468: 1-5
42. Van Wielink JE, Oltmann LF, Leeuwerik FJ, De Hollander JA, Stouthamer AH (1982) A method for in situ characterization of b- and c-type cytochromes in Escherichia coli and Complex III from beef heart mitochondria by combined spectrum deconvolution and potentiometric analysis. Biochim Biophys Acta 681: 177-190
43. Von Haller PD, Donohoe S, Aebersold R, Watts JD (2001) Mass spectrometric characterization of proteins extracted from Jurkat T cell detergent-resistant membrane domains. Eur J Mass Spectrom 7: 181-193
44. Wakefield LM, Cass AEG, Radda GK (1984) Isolation of a membrane protein by chromatofocusing: cytochrome b-561 of the adrenal chromaffin granule. J Biochem Biophys Methods 9: 331-341
45. Widell S, Larsson C (1990) A critical evaluation of marker enzymes used in plasma membrane purification. In: Larsson C, Møller IM (eds) The plant plasma membrane: structure, function and molecular biology. Springer, Berlin Heidelberg New York Tokyo, pp 16-43