Both antisense and sense expression of biotin carboxyl carrier protein isoform 2 inactivates the plastid acetyl-coenzyme A carboxylase in Arabidopsis thaliana

Plant Journal

Volumn 32, Issue 4, 2002, Pages 419-431

  Thelen, Jay J ^a,b   Ohlrogge, John B ^a  

^a MICHIGAN STATE UNIVERSITY   (United States)

^b UNIVERSITY OF MISSOURI   (United States)

Author keywords

Fatty acid biosynthesis; Napin promoter; Oil yeild; Oilseeds; Seed development; Seed setting

Indexed keywords

ANTIBODIES; COENZYMES; FATTY ACIDS; SEED; SYNTHESIS (CHEMICAL);

SEED SETTING;

PLANTS (BOTANY);

ACETYL-COA CARBOXYLASE; ARABIDOPSIS; BIOTINYLATION; CARRIER PROTEINS; FATTY ACIDS; GENE EXPRESSION; GENES, PLANT; ISOENZYMES; PHENOTYPE; PLANT LEAVES; PLASTIDS; PROMOTER REGIONS (GENETICS); RNA, ANTISENSE; SEEDS;

ARABIDOPSIS; ARABIDOPSIS THALIANA; EMBRYOPHYTA; EUKARYOTA; SPERMATOPHYTA;

EID: 0038646820     PISSN: 09607412     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-313X.2002.01435.x     Document Type: Article

References (33)

1. Alonso-Blanco, C., Blankestijn-DeVries, H., Hanhart, C.J. and Koornneef, M. (1999) Natural allelic variation at seed size loci in relation to other life history traits of Arabidopsis thaliana. Proc. Natl Acad. Sci. USA, 96, 4710-4717.
2. Alrefai, R., Berke, T.G. and Rocheford, T.R. (1995) Quantitative trait locus analysis of fatty acid concentrations in maize. Genome, 38, 894-901.
3. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and Struhl, K. (1999) Short Protocols in Molecular Biology, 4th edn. New York: Wiley and Sons, Inc.
4. Baldet, P., Alban, C., Axiotis, S. and Douce, R. (1993) Localization of free and bound biotin in cells from green pea leaves. Arch. Biochem. Biophys. 303, 67-73.
5. Baldet, P., Alban, C. and Douce, R. (1997) Biotin synthesis in plants: purification of bio B gene product equivalent from Arabidopsis thaliana overexpressed in Escherichia coli and its subcellular localization in pea leaf cells. FEBS Lett. 419, 206-210.
6. Chapman-Smith, A., Turner, D.L., Cronan, J.E., Morris, T.W. and Wallace, J.C. (1994) Expression, biotinylation and purification of a biotin-domain peptide from the biotin carboxy carrier protein of Escherichia coli acetyl-CoA carboxylase. Biochem. J. 302, 881-887.
7. Choi, J.-K., Yu F., Wurtele, E.S. and Nikolau, B.J. (1995) Molecular cloning and characterization of the cDNA coding for the biotin-containing subunit of the chloroplastic acetyl-coenzyme A carboxylase. Plant Physiol. 109, 619-625.
8. Elhussein, S.A., Miernyk, J.A. and Ohlrogge, J.B. (1988) Plant holo(acyl carrier protein) synthase. Biochem. J. 252, 39-45.
9. Fatland, B.L., Qian, H., Nikolau, B.J. and Wurtele, E. (1999) Antisense expression of the CAC1 subunit of ACCase in Arabidopsis results in significant modifications in leaf and cell organization. Plant Physiol. Plant Biol. 99 (Suppl.), 78.
10. Focks, N. and Benning, C. (1998) wrinkled1: A novel, low-seed oil mutant of Arabidopsis with a deficiency in the seed-specific regulation of carbohydrate metabolism. Plant Physiol. 118, 91-101.
11. Jako, C., Kumar, A., Wei, Y., Zou, J., Barton, D.L., Griblin, M., Covello, P.S. and Taylor, D.C. (2001) Seed-specific over-expression of an Arabidopsis cDNA encoding diaoylglycerol acyltransferase enhances seed oil content and seed weight. Plant Physiol. 126, 861-874.
12. Mekhedov, S., Martinez de Ilarduya, O. and Ohlrogge, J.B. (2000) Toward a functional catalog of the plant genome: a survey of genes for lipid biosynthesis. Plant Physiol. 122, 389-401.
13. Post-Beittenmiller, M.A., Jaworski, J.G. and Ohlrogge, J.B. (1991) In vivo pools of free and acylated acyl carrier proteins in spinach: evidence for sites of regulation of fatty acid biosynthesis. J. Biol. Chem. 266, 1858-1865.
14. Post-Beittenmiller, M.A., Roughan, G. and Ohlrogge, J.B. (1992) Regulation of plant fatty acid biosynthesis: analysis of acyl-CoA and acyl-acyl carrier protein substrate pools in spinach and pea chloroplast. Plant Physiol. 100, 923-930.
15. Post-Beittenmiller, M.A., Schmid, K.M. and Ohlrogge, J.B. (1989) Expression of holo and apo forms of spinach acyl carrier protein-1 in leaves of transgenic tobacco plants. Plant Cell, 1, 889-899.
16. Roesler, K.R., Shintani, D.K., Savage, L.J., Boddupalli, S. and Ohlrogge, J.B. (1997) Targeting of the Arabidopsis homomeric acetyl-coenzyme A carboxylase to Brassica napus seed plastids. Plant Physiol. 113, 75-81.
17. Roughan, P.G. (1997) Stromal concentrations of coenzyme A and its esters are insufficient to account for rates of chloroplast fatty acid synthesis: evidence for substrate channeling within the chloroplast fatty acid synthase. Biochem. J. 327, 267-273.
18. Sasaki, Y., Hakamada, K., Suama, Y., Nagano, Y., Furusawa, I. and Matsuno, R. (1993) Chloroplast-encoded protein as a subunit of acetyl-CoA carboxylase in pea plant. J. Biol. Chem. 268, 25118-25123.
19. Sasaki, Y., Kozaki, A. and Hatano, M. (1997) Link between light and fatty acid synthesis: thioredoxin-linked reductive activation of plastidic acetyl-CoA carboxylase. Proc. Natl Acad. Sci. USA, 94, 11096-11101.
20. Savage, L.J. and Ohlrogge, J,B. (1999) Phosphorylation of pea chloroplast acetyl-CoA carboxylase. Plant J. 18, 521-527.
21. Shintani, D.K. and Ohlrogge, J.B. (1995) Feedback inhibition of fatty acid synthesis in tobacco suspension cells. Plant J. 7, 577-587.
22. Shintani, D.K., Roesler, K.R., Shorrosh, B.S., Savage, L.J. and Ohlrogge, J.B. (1997) Antisense expression and overexpression of biotin carboxylase in tobacco leaves. Plant Physiol. 114, 881-886.
23. Shorrosh, B.S., Savage, L.J., Soll, J. and Ohlrogge, J.B. (1996) The pea chloroplast membrane-associated protein, IEP96, is a subunit of acetyl-CoA carboxylase. Plant J. 10, 261-268.
24. Stalberg, K., Ellerstrom, M., Josefsson, L.G. and Rask, L. (1993) Deletion analysis of a 2S seed storage protein promoter of Brassica napus in transgenic tobacco. Plant Mol. Biol. 23, 671-683.
25. Sumper, M., Oesterhelt, D., Riepertinger, C. and Lynen, F. (1969) Formation of fatty acids by fatty acid synthetase, a multienzyme complex isolated from yeast. Eur. J. Biochem. 10, 377-387.
26. Thelen, J.J., Mekhedov, S. and Ohlrogge, J.B. (2001) Brassicaceae express multiple isoforms of biotin carboxyl carrier protein in a tissue-specific manner. Plant Physiol. 125, 2016-2028.
27. Thelen, J.J., Miernyk, J.A. and Randall, D.D. (1999) Molecular cloning and expression analysis of the mitochondrial pyruvate dehydrogenase from maize. Plant Physiol. 119, 635-643.
28. Thelen, J.J. and Ohlrogge, J.B. (2002a) Metabolic engineering of fatty acid biosynthesis in plants. Metabolic Engineering J. 4, 12-21.
29. Thelen, J.J. and Ohlrogge, J.B. (2002b) The multisubunit acetyl-CoA carboxylase from pea chloroplasts is associated with envelope membranes in a non-ionic manner through the carboxyl-transferase subunits. Arch. Biochem. Biophys. 400, 245-257.
30. Tissot, G., Douce, R. and Alban, C. (1997) Evidence for multiple forms of biotin holocarboxylase synthetase in pea (Pisum sativum) and in Arabidopsis thaliana: subcellular fractionation studies and isolation of a cDNA clone. Biochem. J. 323, 179-188.
31. Tissot, G., Pepin, R., Job, D., Douce, R. and Alban, C. (1998) Purification and properties of the chloroplastic form of biotin holocarboxylase synthetase from Arabidopsis thaliana overexpressed in Escherichia coli. Eur. J. Biochem. 258, 586-596.
32. Van Hoof, A. and Green, P.J. (1996) Premature nonsense codons decreasethe stability of phytohemaglutinin mRNA in a position-dependent manner. Plant J. 10, 415-424.
33. Vernon, P., Vannier, G. and Arondel, V. (1999) Supercooling capacity of seeds and seedlings in Arabidopsis thaliana. Cryobiology, 39, 138-143.